Alzheimer paired helical filaments (PHFs) studied by high-resolution TEM: what can vertical Pt-C replication tell us about the organization of the pronase-digested PHF core?

Untreated paired helical filaments (PHFs) and pronase-digested PHF-core filaments were stereoscopically imaged with a freeze-drying vertical platinum-carbon replication preparation method for TEM. The untreated PHF have an average wide region (W) = 22.8 +/- 2.4 nm, a narrow region (T) = 10.6 +/- 1.7 nm, and a helical turn period (L) = 78.6 +/- 13.4. The surfaces of the untreated PHF's fuzzy coat appears disorganized. The widths of the pronase-treated PHF-core filaments were significantly reduced (W(d) = 14.8 +/- 1.2 nm, T(d) = 5.7 +/- 1.0 nm, and L(d) = 75.4 +/- 17 nm). The surfaces of the untreated PHF contained approximately 1.1 nm strands, the same size as tau monomer ( approximately 1.0 nm). The pronase-digested PHF cores mostly contained approximately 1.6 +/- 0.3 nm strands although strand diameters ranged from 0.6-2.5 nm. The strands sometimes appear to be wrapped around the filament axis; less often, they appear to be roughly parallel to the PHF axis, and otherwise appear to be randomly oriented. Images of pronase-digested PHF core images are discussed in relation to the core's biochemical composition, its proposed beta structure, and structural subunit models. Images of the untreated and the pronase-digested PHF support a helical ribbon morphology.     

Propagation dynamics of ultra-short high-power laser pulses in air: supercontinuum generation and transverse ring formation

Numerical and semi-analytical results of the propagation of high-power ultra-short near IR laser pulses propagating in ionizing air are presented.     

Covalent functionalization of zinc oxide nanowires for high sensitivity p-nitrophenol detection in biological systems

High-quality zinc oxide (ZnO) nanowires were synthesized using the atmospheric chemical vapor deposition technique and were appropriately characterized. Subsequently, the nanowire surface was covalently grafted with 1-pyrenebutyric acid (PBA) fluorophore, and surface-sensitive X-ray photoelectron spectroscopy and Fourier transform infrared-attenuated total reflectance spectroscopy were utilized to confirm the functionalization of 1-pyrenebutyric acid on the nanowire surface. Additionally, photoluminescence (PL) measurements were used to evaluate the optical behavior of pristine nanowires. Through fluorescence quenching of 1-pyrenebutyric acid by p-nitrophenol, a detection limit of 28 ppb was estimated. Based on these findings, ZnO nanowires functionalized with 1-pyrenebutyric acid are envisaged as extremely sensitive platforms for the ultra-trace detection of p-nitrophenol in biological systems.     

High precision in-cylinder gas thermometry using Laser Induced Gratings: Quantitative measurement of evaporative cooling with gasoline/alcohol blends in a GDI optical engine

The first application of Laser Induced Thermal Gratings Spectroscopy (LITGS) for precision thermometry in a firing GDI optical engine is reported. Crank-angle resolved temperature values were derived from LITGS signals generated in fuel vapour with a pressure dependent precision in the range 0.1–1.0% allowing differences in evaporative or charge cooling effects arising from a variety of ethanol and methanol blends with a model gasoline fuel to be quantified. In addition, fluctuations in temperature arising from cyclic variations in compression were directly detected and measured.     

Collaboration barriers in BIM-based construction networks: A conceptual model

Building information modelling (BIM) teams, hereafter referred to as BIM-based construction networks, are teams whose possibly geographically dispersed members from various organisations and disciplines, perform project tasks on BIM-enabled projects. In recent years, BIM-based construction networks have progressively become the norm in executing BIM activities on projects. However, even though achieving BIM's full capabilities relies on effective collaboration among the team members in BIM-based construction networks, it is still a struggle for these members to collaborate. Nonetheless, only a few studies have been conducted to identify the barriers to strengthening team collaboration in BIM-based construction networks. To address this gap, the current study, by examining 73 journal articles on collaboration in BIM-based construction networks, builds upon a theoretical review of the literature. A conceptual model is presented to capture the main barriers to collaboration in BIM-based construction networks. The study benefits researchers as well as project managers. For researchers, the conceptual model provides an intermediate theory, namely, a theoretical basis to direct further knowledge creation attempts on the topic. In addition, the conceptual model supports project managers on BIM-enabled projects. That is, it simplifies the knowledge now available for practical applications enabling it to be translated into guidelines and practical instructions on real-life projects.     

Stress among South African construction professionals: a job demand-control-support survey

Research into workplace stress in the construction industry has been dominated by studies undertaken in Hong Kong, England and Australia, with relatively little attention having been paid to African countries. A purposively selected sample of thirty-six construction professionals (comprising architects, project managers, construction managers, engineers and quantity surveyors) based in Cape Town, South Africa, were surveyed regarding their perceptions about, and experiences of, workplace stress. The purpose was to identify and rank job demand, job control and job support factors and to analyse these by gender and professional grouping, both in terms of how respondents perceived them, as well as in terms of how frequently they had experienced them. The main finding was that respondents’ perceptions about the importance of job demand, job control and job support factors were largely consistent with their own experience of these factors. Distinguishing between perceptions and experience of stressors and moderators of stress was, however, considered valuable because the fact that differences were found indicates the need for precision in the design of scales. The highest ranked factors were, respectively, ‘critical time constraints’, ‘volume of work’ and ‘adequate compensation (salary)’. Women and men ranked items differently, as did the various professional groups. The main conclusions were that the construction project environment is a time-pressured, complex, environment in which work-life balance is negatively affected, particularly for women. Control over the type, flow and volume of work were perceived to be the main moderators of stress, whilst salary and career path opportunities were perceived to be the main job support moderators of stress. The applicability of using the job demand–control–support framework was confirmed given that the main stressors, control and support moderators were found to be to be largely consistent with the findings of previous studies. Future research taking a gendered approach should anticipate issues important to female respondents and should consider the benefits of qualitative methods. The design of research focusing on construction professionals should consider using the project team as the unit of analysis to ensure that the influence of the interconnectedness of participants’ roles is taken into account.     

Condom use by South African construction workers

Consistent and proper condom use is pivotal in preventing HIV infection. HIV prevalence in South Africa is among the highest in the world, with the construction industry especially affected, yet little is known about condom use by construction workers and the determinants thereof. Data were gathered from 512 site-based workers in the Western Cape. A theoretical model explaining condom use as a function of demographic factors, HIV knowledge, substance usage and risky sexual behaviour (excluding condom use) was proposed and tested using regression and structural equations modelling. The findings indicated that age, gender and level of education were indirect determinants of condom use, with higher levels of education predicting better AIDS-related knowledge. Higher levels of risky sexual behaviour were associated with more frequent use of condoms, suggesting greater awareness of risk. Condom use was adversely affected by greater alcohol and drug use, probably as a result of diminished capacity to assess risk. Finally, lower and not higher levels of AIDS-related knowledge were associated with better condom use, suggesting that improved knowledge of HIV transmission may work contrary to safe sex practice if it lulls the person into a false sense of confidence. Recommendations for targeted workplace interventions are proposed.     

Easing the implementation and change of manufacturing software systems

The software systems that underpin contemporary manufacturing enterprises can become the single most inflexible part of an enterprise, constraining a business that has to evolve. This paper describes an approach to manufacturing software system creation which supports system evolution. It is based on the notion that raising the level of the computing infrastructure through the use of a domain machine, can remove the need to transform the clear abstractions and decomposition identified during design into the incomprehensible maze of programming language constructs which prove difficult to maintain.

The paper provides a comparison between two implementations of a system to control a printed circuit board manufacturing line, one based on a conventional approach and the other based on the authors manufacturing domain machine. Ease of change enabled by the domain machine is illustrated through measuring the degree of modification required to each system when incorporating a new solder paste print machine in the production line.     

Images of native Americans

Abstract

This interesting volume explores the photographic environment and output of four Anglo women who had extended photographic relationships with Native American people: Kate Cory, an artist and collector who lived with the Hopi people for many years; Mary Shaffer; who travelled amongst the Stoney and Metis of Alberta, Canada; Jane Gay, who assisted ethnographer Alice Fletcher and documented land allotment and the effects of the Dawes Act on the Nez Percé people; Grace Nicholson, who set up commercial enterprises trading in art and cultural objects. None of the women was primarily a photographer. Their photographic work was always an adjunct to other purposes, to record encounters commercial, artistic or ethnographic, but at the same time the work functioned as a key articulation of their relationships with Native peoples. All four women, in their different ways, looked to Native American peoples as a way of defining and experiencing their own identities and spirituality and all four shared a commitment to and engagement with these peoples.     

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CARING FOR THE UNEMPLOYED. Prepared for the Association of Metro‐polital Authorities by Susan Balloch, Chris Hume, Brian Jones and Peter Westland. Publishers: Bedford Square Press, London, 1985, for the National Council for Voluntary Organisations.

THE LONG‐TERM UNEMPLOYED. ACTION FOR A FORGOTTEN MILLION, by Peter Ashby (Head, NVCO Policy Analysis Unit). An NCVO Policy Discussion document, Bedford Square Press, London (1985).

ANTHROPOGENIC COMPOUNDS: Volume 3 Part C of The Handbook of Environmental Chemistry, edited by O. Hutzinger, Springer‐Verlag, Germany, 248 pp. 1986. (DM 188.00)

HEAVY METALS IN NATURAL WATERS: APPLIED MONITORING AND IMPACT ASSESSMENT, by James W. Moore and S. Ramamoorthy, Springer‐Verlag, U.S.A. and Germany, viii #pl 268 pages, index, 1984. (DM 108.00)

DESTRUCTION AND DISPOSAL OF PCBs BY THERMAL AND NON‐THERMAL METHODS, by D. G. Ackerman, L. L. Scinto, P. S. Bakshi, R. G. Delumyea, R. J. Johnson, G. Richard, A. M. Takata and E. M. Sworzyn. Noyes Data Corporation, Park Ridge, New Jersey, 1983, xiv #pl 417 pages (no index). (#dl48.00)

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SAFE DRINKING WATER. THE IMPACT OF CHEMICALS ON A LIMITED RESOURCE, edited by R. G. Rice, J. Wiley and Sons, Chichester, U.K., Lewis Publishers Inc. Chelsea MI, 1985. (#bp36.00)

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REGULATED RIVERS, edited by A. Lillehammer and S. J. Saltveit, Univer‐sitetsforlaget AS, Oslo, 1984. (#bp47.50)

NUTRIENT CYCLING IN TROPICAL FOREST ECOSYSTEMS, by C. F. Jordan, Wiley, Chichester, 1985. (#bp13.45)