Homology and functional similarity of an hrp-linked pathogenicity locus, dspEF, of Erwinia amylovora and the avirulence locus avrE of Pseudomonas syringae pathovar tomato

The "disease-specific" (dsp) region next to the hrp gene cluster of Erwinia amylovora is required for pathogenicity but not for elicitation of the hypersensitive reaction. A 6.6-kb apparent operon, dspEF, was found responsible for this phenotype. The operon contains genes dspE and dspF and is positively regulated by hrpL. A BLAST search revealed similarity in the dspE gene to a partial sequence of the avrE locus of Pseudomonas syringae pathovar tomato. The entire avrE locus was sequenced. Homologs of dspE and dspF were found in juxtaposed operons and were designated avrE and avrF. Introduced on a plasmid, the dspEF locus rendered P. syringae pv. glycinea race 4 avirulent on soybean. An E. amylovora dspE mutant, however, elicited a hypersensitive reaction in soybean. The avrE locus in trans restored pathogenicity to dspE strains of E. amylovora, although restored strains were low in virulence. DspE and AvrE are large (198 kDa and 195 kDa) and hydrophilic. DspF and AvrF are small (16 kDa and 14 kDa) and acidic with predicted amphipathic alpha helices in their C termini; they resemble chaperones for virulence factors secreted by type III secretion systems of animal pathogens.     

Medical challenges of internal conflicts

The most prevalent menace since the end of the cold war is the occurrence of civil wars and local and regional conflicts. The term "low intensity conflict" describes the new threat environment and covers a multitude of phenomena, such as civil wars, guerrilla warfare, terrorism and counterinsurgency operations occurring between routine, peaceful inter- or intrastate competition, and a sustained conventional conflict. There is a great challenge to alert the physicians in general, and the surgical community of the world in particular, to the new threat environment and the medical challenges involved in treating casualties of low intensity conflicts. Specifically, a new international body of surgeons might be required to coordinate the recruitment, training, and creditation for surgeons with special expertise in the management of victims of such conflicts and to facilitate research and general knowledge of the medical challenges of modern conflicts.     

Spatiotemporal imaging of human brain activity using functional MRI constrained magnetoencephalography data: Monte Carlo simulations

The goal of our research is to develop an experimental and analytical framework for spatiotemporal imaging of human brain function. Preliminary studies suggest that noninvasive spatiotemporal maps of cerebral activity can be produced by combining the high spatial resolution (millimeters) of functional MRI (fMRI) with the high temporal resolution (milliseconds) of electroencephalography (EEG) and magnetoencephalography (MEG). Although MEG and EEG are sensitive to millisecond changes in mental activity, the ability to resolve source localization and timing is limited by the ill-posed "inverse" problem. We conducted Monte Carlo simulations to evaluate the use of MRI constraints in a linear estimation inverse procedure, where fMRI weighting, cortical location and orientation, and sensor noise statistics were realistically incorporated. An error metric was computed to quantify the effects of fMRI invisible ("missing") sources, "extra" fMRI sources, and cortical orientation errors. Our simulation results demonstrate that prior anatomical and functional information from MRI can be used to regularize the EEG/MEG inverse problem, giving an improved solution with high spatial and temporal resolution. An fMRI weighting of approximately 90% was determined to provide the best compromise between separation of activity from correctly localized sources and minimization of error caused by missing sources. The accuracy of the estimate was relatively independent of the number and extent of the sources, allowing for incorporation of physiologically realistic multiple distributed sources. This linear estimation method provides an operator-independent approach for combining information from fMRI, MEG, and EEG and represents a significant advance over traditional dipole modeling.     

Measurements on a Dynamic A-B Phase Boundary in Superfluid 3He at Very Low Temperatures

We present preliminary measurements of the dynamics of a moving A-B phase interface in superfluid ³ He at temperatures below 0.2T _c We initially stabilise the interface at low temperatures with a shaped magnetic field. We can then move the interface in a controlled manner by applying small additional time-dependent fields. The interface is created inside a quasiparticle radiator consisting of a cylindrical chamber in weak thermal contact with the refrigerant. Vibrating wire resonators inside the radiator allow us to monitor the temperature of the superfluid and to infer the heat generated by the interface motion. When we oscillate the interface at low frequencies, we measure spectacular oscillatory swings of the liquid temperature arising from the enormous change in the low lying density of states as the volume of the A-phase superfluid is alternately compressed and expanded. We have also observed hysteresis in the transition as a function of magnetic field. In particular, we observe a small history-dependent super-magnetisation of the B-phase prior to A-phase nucleation in the experimental chamber. When the system is in the metastable super-magnetised state we are able to observe a higher nucleation probability of the A-phase when the cryostat is exposed to neutrons.     

Digital image processing-an object-oriented approach

This paper presents a class library dedicated to digital image processing which provides resources for essential digital image processing operations. Besides its practical value, the class library is used to teach both object-oriented software engineering methodology and digital image processing. The resulting module can be accessed from independent specialized applications through its public interface while its implementation details are perfectly encapsulated. First of all it is used as the inner part of an integrated environment designed for students and researchers in the field of digital image processing. This software component was built aiming for both portability and extendibility. It contains attributes and methods that allow for a wide range of digital image processing algorithms: point and geometric operations; filter, edge detection, segmentation and texture operations; and fast Fourier transforms, discrete cosine transforms, and wavelet and Gabor transformations. All these can be used for image enhancement, restoration, compression, analysis, and synthesis     

New aspects in the microstructure of YBCO melt-textured high-Tc superconductors

The microstructure and phase constitution of bulk textured YBCO samples prepared by the modified MTG method in the longitudinal temperature gradient have been investigated. We found the existence of an unusual phase between stacked platelets. Microanalysis shows that this phase may be characterized as Y-143. This phase forms ellipsoid-like particles with a typical size about 0.05–2 which can play the role of effective pinning centers. The reason for the phase formation is discussed. We assume that this phase exists commonly in melt-textured samples prepared in the temperature field gradient.     

Surgical anatomy for endoscopic subfascial division of perforating veins

PURPOSE: This study was undertaken to define the surgical anatomy of the medial perforating veins (PVs) of the leg and to provide information on how to gain access to all medial PVs from the superficial posterior compartment during a subfascial endoscopic procedure. METHODS: The venous anatomy of 40 limbs (from 23 cadavers) were studied. Medial PVs located between the ankle and the tibial tuberosity were dissected. None of the subjects had pathologic evidence of venous disease. Each PV's type (direct or indirect), size (< 1 mm, 1 to 2 mm, > 2 mm), location (distances from ankle [D1], and tibia [D2]), and accessibility from the superficial posterior compartment were recorded. RESULTS: Five hundred fifty-two PVs were identified (mean, 13.8; range, 7 to 22). Two hundred eighty-seven PVs (52%) directly connected the superficial with the deep systems, 228 (41%) were indirect muscle perforators, and 37 PVs (7%) were undetermined. One hundred thirty-seven PVs (25%) were > 2 mm. Sixty-three percent of PVs were accessible from the superficial posterior compartment. In the distal half of the leg, two groups of direct PVs could be identified (Cockett II: D1, 7 to 9 cm; Cockett III: D1, 10 to 12 cm). In the proximal half of the leg, paratibial direct PVs (D2 < or = 1 cm) were found clustered in three groups (D1, 18 to 22 cm; D1, 23 to 27 cm; D1, 28 to 32 cm). CONCLUSIONS: Our study confirmed the presence of the Cockett II and III PVs and three groups of proximal paratibial PVs, including the "24-cm" perforators. Two thirds of the medial direct PVs are accessible for endoscopic division from the superficial posterior compartment. To divide paratibial PVs, however, incision of the paratibial deep fascia is frequently required.     

Ultrarapid metabolizers of debrisoquine: characterization and PCR-based detection of alleles with duplication of the CYP2D6 gene

During a six-month period 2,221 haemocultures obtained from patients hospitalized in the Faculty Hospital Olomouc were examined. In all 304 isolated bacteria the sensitivity was assessed by the standard dilution micromethod and moreover all positive haemocultures were examined the "direct" disc method. Agreement between the results of the two methods was proved in 84% of pairs of tests and within a range from 67 to 100%, depending on the type of antimicrobial preparation. Based on these findings it may be stated that assessment of the sensitivity by the "direct" method agrees significantly with assessment of the sensitivity according to minimal inhibitory concentrations (MIC). In patients with septicaemia this procedure makes it possible to change empirical antibiotherapy by 24 hours sooner to aimed therapy.     

The feasibility of general task systems with precedence constraints on multiprocessor platforms

Feasibility analysis determines (prior to system execution-time) whether a specified collection of hard-real-time jobs executed on a processing platform can meet all deadlines. In this paper, we derive near-optimal sufficient tests for determining whether a given collection of jobs can feasibly meet all deadlines upon a specified multiprocessor platform assuming job migration is permitted. The collection of jobs may contain precedence constraints upon the order of execution of these jobs. The derived tests are general enough to be applied even when the collection of jobs is incompletely specified. We discuss the applicability of these tests to the scheduling of collections of jobs that are generated by systems of recurrent real-time tasks. We also show that our feasibility conditions may be used to obtain global-EDF schedulability conditions.