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111.
112.
MJ Welters AM Fichtinger-Schepman RA Baan MJ Flens RJ Scheper BJ Braakhuis 《Canadian Metallurgical Quarterly》1998,77(4):556-561
Spontaneous proliferative liver lesions were found in 15 (13 males and 2 females) of 244 (122 of each sex) transgenic (Tg) mice carrying the human prototype c-H-ras gene (rasH2). The liver lesions included 3 foci of cellular alteration, 1 hepatocellular adenoma, 5 hepatocellular carcinomas, and 4 hepatic hemangiosarcomas in the males and 1 focus of cellular alteration and 1 hepatocellular carcinoma in the females. The mutation patterns of the human and endogenous mouse c-H-ras codon 61 in these proliferative liver lesions were analyzed by DNA amplification using polymerase chain reaction, single-strand conformation polymorphism (PCR-SSCP), and oligonucleotide dot blot hybridization. The hepatocellular carcinomas in 4 males and 1 female contained a point mutation in the mouse c-H-ras gene: 3, 1, and 1 carcinomas had a CAA to AAA transversion at the first base of codon 61, a CAA to CTA transversions, and a CAA to CGA transition at the second base of codon 61, respectively. No point mutations in the human c-H-ras transgene were detected in any hepatocellular carcinoma. All 4 hepatic hemangiosarcomas had a CAG to CTG transversion at codon 61 of the human c-H-ras gene, but no point mutations were detected in codon 61 of the mouse c-H-ras gene. No mutations in human or mouse c-H-ras codon 61 were detected in altered cell foci or hepatocellular adenoma. These results indicate that spontaneous liver tumors in rasH2 Tg mice contain different mutation patterns depending on the histologic type or cell origin of the tumors (i.e., hepatocellular carcinomas or hepatic hemangiosacomas). The absence of similar mutations in foci of cellular alteration and the hepatocellular adenoma suggests that the occurrence of codon 61 point mutations is a late event in the progression of hepatocellular neoplasia in rasH2 Tg mice. 相似文献
113.
It has been previously reported that Bryostatin 1 (Bryo1) induces differentiation of the human acute lymphoblastic leukemia (ALL) cell line, Reh, to a monocytoid B-cell stage. In this study we demonstrate that a novel protein, ubiquitin COOH-terminal hydrolase (UCH-L1), is associated with this differentiation. Reh cells were treated with 200 nmol/l of Bryo1 for 72 h and analyzed for changes in morphology, surface immunophenotype, acid phosphatase and terminal deoxynucleotidyl transferase. Protein patterns of the parent and differentiated cells, by two-dimensional polyacrylamide gel electrophoresis (2D PAGE), were studied. Bryo1-treated cells expressed morphologic, phenotypic and enzymatic features of the monocytoid B-cell stage. The UCH-L1 enzyme (MW-pl 34-5.3) was detected by 2 D PAGE in the differentiated, but not in parent cells. The presence of UCH-L1 in the Bryo1-treated cells was further confirmed by immunoblotting of 2 D PAGE using UCH-L1 polyclonal antibody. Ubiquitin expression was studied in parent and Bryo1-treated cells and was compared with 12-O-tetradecanoylphorbol-13-acetate (TPA)-treated cells. Both agents, TPA and Bryo1, increased the level of ubiquitin expression as detected by flow cytometry. Sodium borohydride, an inhibitor of UCH-L1, inhibited the Bryo1-induced differentiating effect on Reh cells. To date, the mechanism by which Bryo1, exerts its B-cell differentiating effect is not fully understood. This study shows that UCH-L1 expression may play a major role in Bryo1-induced differentiation in pre-B-ALL. 相似文献
114.
Constructions of particular random processes 总被引:1,自引:0,他引:1
Johnson G.E. 《Proceedings of the IEEE. Institute of Electrical and Electronics Engineers》1994,82(2):270-285
This paper reviews how to construct sets of random numbers with particular amplitude distributions and correlations among values. These constructions support both high-fidelity Monte Carlo simulation and analytic design studies. A variety of constructions is presented to free engineering models from “white or normal” limitations embodied in many current simulations. The methods support constructions of conventional stationary and normally distributed processes, nonstationary, nonnormal signal and interference waveforms, nonhomogeneous random scenes, nonhomogeneous volumetric clutter realizations, and snapshots of randomly evolving, nonhomogeneous scenes. Each case will have specified amplitude statistics, e.g., normal, log-normal, uniform, Weibull, or discrete amplitude statistics; and selected correlation, e.g., white, pink, or patchy statistics, clouds. or speckles. Sets of random numbers with correlation, nonstationarities, various tails for the amplitude distributions, and multimodal distributions can be constructed. The paper emphasizes aspects of probability theory necessary to engineering modeling 相似文献
115.
116.
When planning an SDH network, explicit structure, such as rings or a network hierarchy, is often imposed to allow for easier network protection and management. Decisions on node connectivity are also heavily dependent on available transmission capacity and network geography, as well as the demands placed on the SDH transport layer. The strictly imposed structure therefore makes it unlikely for unplanned properties to appear but here we describe how the BT SDH network exhibits emergent power-law properties in a range of metrics. These properties are similar to those previously found in the Internet, but the Internet in contrast is not globally planned, has adaptive elements such as dynamic routing and peering agreements, lacks explicit imposed structure, and is less coupled to transmission topologies.This paper shows that even with a wide range of restrictions and controls the BT SDH network topology follows power-laws and we offer possible sources for them, concentrating on the possible effect of adjacent network layers. The existence of these properties has wide implications in network modelling, as well as network scaling, growth, and robustness analysis. 相似文献
117.
The contrasting agent Gd-DTPA (Magnevist, Schering AG, Germany) was tested when used in magnetic resonance tomography using extra-low (0.04 T) magnetic field intensity in 68 patients with various brain and spine tumors. The agent was injected intravenously in a dose of 0.1 mmol/kg. No complications were found. The tests showed its high efficiency in the diagnosis of brain neoplasms (particularly in malignant intracerebral disorders, meningiomas and neurinomas). Benign astrocytomas induced no noticeable signal intensification on post-contrasting T1-weighed tomograms. In all cases of spinal tumors, the application of Gd-DTPA could specify the extent of tumor invasions, the presence of cysts and identify accessory tumor nodes in one case. 相似文献
118.
Designing circuits with partial scan 总被引:1,自引:0,他引:1
Agrawal V.D. Kwang-Ting Cheng Johnson D.D. Sheng Lin T. 《Design & Test of Computers, IEEE》1988,5(2):8-15
In this scan design methodology, only selected faults are targeted for detection. These faults are those not detected by the designer's functional vectors. The test generator decides exactly which flip-flops should be scanned using one of two methods. In the first method, all possible tests are generated for each target fault, and the set of tests requiring the fewest flip-flops is selected. In the second method, only one test is generated for each fault, and the use of flip-flops is avoided as much as possible during test generation. Examples of actual VLSI circuits show a savings of at least a 40% in full-scan overhead 相似文献
119.
J. D. Russell J. Mumley M. Spring A. Kutz R. N. Johnson 《Journal of Automated Methods and Management in Chemistry》1991,13(3):115-117
To measure the content uniformity of conjugated cestrogens tablets,
the USP XXII monograph specifies a capillary gas chromatographic method. This involves separation of the trimethylsilyl derivatives of the various oestrogens found in a typical conjugated oestrogens tablet using a fused-silica 0V 225 bonded phase column. Hydrogen is used as the carrier gas with FID detection. A more rapid spectrophotofluorometric method of analysis has been developed in which the oestrogens are extracted from the dosage form into water, then transferred into organic solvent as the dicyclohexylamine complex. Fluorescence is developed by heating the solution of the complex in the presence of 70% sulphuric acid. The chemistry and detection are carried out using a segmented-flow analyser.The spectrophotofluorometric method is preferred as a routine control for content uniformity because considerable time is saved during sample preparation and analysis compared to gas chromatography. Coefficients of variation show that both methods produce
acceptable results. These results fall well within the USP XXII [1] monograph, limits and also well within the tighter limits imposed by the USP XXII, section 905. 相似文献
120.