全文获取类型
收费全文 | 25343篇 |
免费 | 461篇 |
国内免费 | 19篇 |
专业分类
电工技术 | 230篇 |
综合类 | 16篇 |
化学工业 | 2724篇 |
金属工艺 | 242篇 |
机械仪表 | 355篇 |
建筑科学 | 930篇 |
矿业工程 | 44篇 |
能源动力 | 466篇 |
轻工业 | 2038篇 |
水利工程 | 265篇 |
石油天然气 | 29篇 |
无线电 | 1793篇 |
一般工业技术 | 2483篇 |
冶金工业 | 11270篇 |
原子能技术 | 167篇 |
自动化技术 | 2771篇 |
出版年
2023年 | 70篇 |
2022年 | 159篇 |
2021年 | 285篇 |
2020年 | 145篇 |
2019年 | 211篇 |
2018年 | 284篇 |
2017年 | 263篇 |
2016年 | 286篇 |
2015年 | 259篇 |
2014年 | 415篇 |
2013年 | 701篇 |
2012年 | 711篇 |
2011年 | 974篇 |
2010年 | 675篇 |
2009年 | 713篇 |
2008年 | 747篇 |
2007年 | 704篇 |
2006年 | 595篇 |
2005年 | 585篇 |
2004年 | 536篇 |
2003年 | 493篇 |
2002年 | 438篇 |
2001年 | 355篇 |
2000年 | 334篇 |
1999年 | 626篇 |
1998年 | 3876篇 |
1997年 | 2286篇 |
1996年 | 1504篇 |
1995年 | 863篇 |
1994年 | 750篇 |
1993年 | 831篇 |
1992年 | 249篇 |
1991年 | 247篇 |
1990年 | 249篇 |
1989年 | 241篇 |
1988年 | 238篇 |
1987年 | 172篇 |
1986年 | 172篇 |
1985年 | 231篇 |
1984年 | 142篇 |
1983年 | 108篇 |
1982年 | 127篇 |
1981年 | 153篇 |
1980年 | 139篇 |
1979年 | 95篇 |
1978年 | 93篇 |
1977年 | 267篇 |
1976年 | 440篇 |
1975年 | 89篇 |
1974年 | 74篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
61.
62.
63.
AM Pollock 《Canadian Metallurgical Quarterly》1995,310(6994):1580-1584
The Association of Metropolitan Authorities has recently proposed that responsibility for the NHS should pass from health authorities to local authorities. One of the fiercest debates at the outset of the NHS was whether the hospitals should be run by local authorities. In the end the minister for health, Aneurin Bevan, decided against local democracy and in favour of a national health service. His arguments included the fact that equality of treatment could not be guaranteed if facilities varied with local finances and that even the largest authorities were not big enough to pool risks and expertise. All these arguments still apply today, and the recent changes in community care provide an insight into how a market model of local authority control might work. The changes have been accompanied by a shift from public to private sector provision and the introduction of charges for services that the NHS once provided free. As important, the willingness and ability of local authorities to raise extra revenue from local taxes and charges affect the service they can provide, so leading to inequalities of provision. Local authorities have yet to make the case that they can preserve the fundamental principles and benefits of the NHS, including its reliance on central taxation and unified funding formulas. 相似文献
64.
Comparison of protein adsorption isotherms and uptake rates in preparative cation-exchange materials
Adsorption isotherms and effective diffusivities of lysozyme in a set of six preparative cation-exchange stationary phases were determined from batch uptake data in a stirred vessel. Both a pore diffusion and a homogeneous diffusion model were used in estimating diffusivities, with the isotherms fitted to a non-Langmuirian analytical isotherm equation. The capacities inferred from the isotherms are found to be correlated with the surface area accessible to lysozyme, the effective surface concentrations obtained being in agreement with values measured by different methods in various non-chromatographic systems. The pore diffusivities show systematic trends with protein and salt concentration, and effects of pore size and connectivity are also evident. Some trends in the homogeneous diffusivities are quite different to those in the pore diffusivities, but these differences largely disappear when the homogeneous diffusivities are rescaled to account for adsorption equilibrium behavior. Additional information is required to elucidate further the mechanisms of coupled diffusion and adsorption in stationary phases. 相似文献
65.
J Booij RJ Knol L Reneman K de Bruin AG Janssen EA van Royen 《Canadian Metallurgical Quarterly》1998,25(12):1666-1669
Flavobacterium aurantiacum NRRL B-184 possesses the ability to degrade aflatoxin B1 in solution and in several food items. Aflatoxin B1 is a potent carcinogen that causes significant economic losses to the agricultural and food industry. The role of trace metal ions (Cu2+, Mn2+, Zn2+, and Co2+) were studied in an effort to understand the enzymatic system involved in aflatoxin B1 degradation by F aurantiacum. The effect of divalent chelators (EDTA and 1,10-phenanthroline [OPT]) in the presence of the trace metal ions was studied as well. Aflatoxin B1 (10 microg/ml) was added to 72-h cultures of F aurantiacum that had been washed and resuspended in phosphate buffer (pH 7.0). HPLC was used to determine aflatoxin B1 concentration in these cultures. Incubating cells at 30 degrees C with 1 and 10 mM Cu2+, Mn2+, and Zn2+ significantly decreased aflatoxin B degradation after 4 and 24 h (P < 0.05). Decreased degradation was also observed with 1 and 10 mM Cu2+ and Zn2+ after 48 h and with 0.1 mM Cu2+ after 24 and 48 h. Co2+ did not have a significant effect on aflatoxin B1 degradation. EDTA and OPT did not counter the inhibition in the presence of Cu2+. The addition of 1 mM EDTA countered the inhibition by 1 mM Mn2+ after 4 and 24 h, but 1 mM OPT did not counter the inhibition by 10 mM Mn2+ after 4 and 24 h. OPT countered the inhibition by 1 mM Zn2+ after 4 and 48 h. These trace elements inhibit aflatoxin B1 degradation by F aurantiacum. In addition, their presence necessitates higher concentrations (>1 mM) of EDTA and OPT for the removal of their inhibitory effect. 相似文献
66.
67.
W. F. J. Sampers A. P. G. G. Lamers A. A. van STEENHOVEN 《Chemical Engineering Communications》1993,125(1):187-196
In many applications swirling flow is used to enhance heat and mass transfer. One of the problems of modelling a turbulent swirling flow is the choice of the turbulent closure model which is acceptable for engineering purposes. To evaluate which model performs best, numerical results are compared with experimental data. Local velocity measurements are carried out on a swirling flow in a circular tube. The measurement method is hot-wire anemometry combined with visualization techniques. The numerical analysis is carried out using the k-ε model and the Algebraic Stress Model. In the experiment a symmetrical swirl was observed, Comparing the experimental data with the numerical results shows that the Algebraic Stress Model represents the experimental data quite well, whereas the k-ε model fails. 相似文献
68.
A Pierres H Feracci V Delmas AM Benoliel JP Thiery P Bongrand 《Canadian Metallurgical Quarterly》1998,95(16):9256-9261
We describe a method allowing quantitative determination of the interaction range and association rate of individual surface-attached molecules. Spherical beads (1.4 micro(m) radius) were coated with recombinant outer domains of the newly described classical type II cadherin 11, a cell adhesion molecule. Beads were driven along cadherin-coated surfaces with a hydrodynamic force of approximately 1 pN, i.e., much less than the mechanical strength of many ligand-receptor bonds. Spheres displayed periods of slow motion interspersed with arrests of various duration. Particle position was monitored with 50 Hz frequency and 0.025 micro(m) accuracy. Nearly 1 million positions were recorded and processed. Comparison between experimental and computer-simulated trajectories suggested that velocity fluctuations might be related quantitatively to Brownian motion perpendicular to the surface. The expected amplitude of this motion was of order of 100 nm. Theoretical analysis of the relationship between sphere acceleration and velocity allowed simultaneous determination of the wall shear rate and van der Waals attraction between spheres and surface. The Hamaker constant was estimated at 2.9 x 10(-23) J. The frequency of bond formation was then determined as a function of sphere velocity. Experimental data were consistent with the view that the rate of association between a pair of adhesion molecules was approximately 1.2 x 10(-3) s-1 and the interaction range was approximately 10 nm. It is concluded that the presented methodology allows sensitive measurement of sphere-to-surface interactions (with approximately 10 fN sensitivity) as well as the effective range and rate of bond formation between individual adhesion molecules. 相似文献
69.
Oligonucleotide N3'-->P5' phosphoramidates (3'-phosphoramidates) are DNA analogs that are presently under investigation as potential therapeutic agents. These compounds may also hold promise as a diagnostic tool. Here, we describe a rapid method for the analysis of single-stranded RNA fragments utilizing 3'-phosphoramidate oligonucleotides as probes in conjunction with capillary gel electrophoresis (CGE). 3'-Phosphoramidate 9-mers were mixed with complimentary RNA, and CGE was used to monitor duplex formation. Complimentary strands of RNA and 3'-phosphoramidate formed duplexes that gave unique relative mobilities based on an internal standard. The ability of CGE to discriminate between perfect duplexes and duplexes that contain a base mismatch was also investigated. However, the primary focus of this work was to determine CGE's ability to detect the presence of the 3'-phosphoramidates/RNA duplex under routine electrophoretic running conditions. Polyacrylamide gel electrophoresis analysis was utilized to verify duplex formation. 相似文献
70.
F Nachon L Ehret-Sabatier D Loew C Colas A van Dorsselaer M Goeldner 《Canadian Metallurgical Quarterly》1998,37(29):10507-10513
Purified butyrylcholinesterase (BuChE) was photolabeled by [3H]-p-N, N-dimethylamino benzene diazonium ([3H]DDF) to identify the quaternary ammonium binding sites on this protein [Ehret-Sabatier, L. , Schalk, I., Goeldner, M., and Hirth, C. (1992) Eur. J. Biochem. 203, 475-481]. The covalent photoincorporation occurs with a stoichiometry of one mole of probe per mole of inactivated site and could be fully prevented by several cholinergic inhibitors such as tacrine or tetramethylammonium. After complete deglycosylation of the enzyme using N-glycosidase F, the alkylated protein was trypsinolyzed and the digests were analyzed by HPLC coupled to ES-MS. A direct comparison of tryptic fragments from labeled and unlabeled BuChE allowed us to identify the tryptic peptide Tyr61-Lys103 as carrying the probe. Purification of the labeled peptides by anion-exchange chromatography gave a major radioactive peak which was further fractionated by reversed-phase HPLC leading to three, well-resolved, radioactive peaks. Microsequencing revealed that two of these peaks contained an overlapping sequence starting at Tyr61, while the third peak contained a sequence extending from Thr315. Radioactive signals could be unambiguously attributed to positions corresponding to residues Trp82 and Tyr332. This labeling study establishes the existence of two different binding domains for quaternary ammonium in BuChE and exemplifies additional cation/pi interactions in cholinergic proteins. This work strongly supports the existence of a peripheral anionic site in BuChE, implying residue Tyr332 as a key element. 相似文献