全文获取类型
收费全文 | 1760篇 |
免费 | 0篇 |
专业分类
电工技术 | 3篇 |
化学工业 | 47篇 |
金属工艺 | 4篇 |
机械仪表 | 15篇 |
矿业工程 | 6篇 |
轻工业 | 6篇 |
水利工程 | 3篇 |
石油天然气 | 5篇 |
无线电 | 8篇 |
一般工业技术 | 132篇 |
冶金工业 | 1520篇 |
原子能技术 | 4篇 |
自动化技术 | 7篇 |
出版年
2022年 | 5篇 |
2021年 | 4篇 |
2020年 | 5篇 |
2019年 | 5篇 |
2018年 | 6篇 |
2017年 | 7篇 |
2016年 | 10篇 |
2014年 | 12篇 |
2013年 | 7篇 |
2012年 | 11篇 |
2011年 | 7篇 |
2010年 | 12篇 |
2009年 | 9篇 |
2008年 | 18篇 |
2007年 | 12篇 |
2006年 | 14篇 |
2005年 | 9篇 |
2004年 | 8篇 |
2003年 | 9篇 |
2002年 | 8篇 |
2001年 | 6篇 |
1999年 | 29篇 |
1998年 | 450篇 |
1997年 | 256篇 |
1996年 | 181篇 |
1995年 | 145篇 |
1994年 | 86篇 |
1993年 | 80篇 |
1992年 | 19篇 |
1991年 | 19篇 |
1990年 | 16篇 |
1989年 | 9篇 |
1988年 | 13篇 |
1987年 | 10篇 |
1986年 | 7篇 |
1985年 | 11篇 |
1984年 | 5篇 |
1983年 | 7篇 |
1982年 | 7篇 |
1981年 | 17篇 |
1980年 | 20篇 |
1979年 | 2篇 |
1978年 | 10篇 |
1977年 | 60篇 |
1976年 | 100篇 |
1975年 | 2篇 |
1974年 | 2篇 |
1973年 | 2篇 |
1966年 | 2篇 |
1958年 | 2篇 |
排序方式: 共有1760条查询结果,搜索用时 0 毫秒
61.
AV Demin AV Kolesnikov MA Olfer'ev PV Favorov KV Fegeding AG Gabibov NV Gnuchev 《Canadian Metallurgical Quarterly》1998,34(10):1333-1337
A new system for the expression of a catalytic light chain antibody to the vasoactive intestinal peptide is described. The system made possible the isolation the large amounts of a homogeneous protein without any additional peptide domains. The preparation obtained can be used in further experiments on light chain crystallization and in X-ray-structural analysis of its catalytic center. 相似文献
62.
AV Goriunova GV Kozlovskaia IA Kozlova NV Rimashevskaia NIu Savvateeva GN Shimonova 《Canadian Metallurgical Quarterly》1996,96(4):46-50
Computed tomography (CT) was performed in 189 individuals: in 44 children of schizophrenic parents (high risk group, HRG), in 39 parents with schizophrenia or with schizophrenic disturbance, in 56 children with schizophrenia, in 50 children with consequences of early organic damages of central nervous system (mental retardation syndrome and generalized tic syndrome). The frequency of CT changes was equal in the mentioned groups but their character was quite different. The widening of brain's liquor system (89.7%), the signs of frontal and temporal atrophia (31%), foci of decreased density of cerebral brain's matter, closer in subcortical ganglia and periventricular zone, and different anomalies of brain were observed in HRG children. 相似文献
63.
SH Willis AH Rux C Peng JC Whitbeck AV Nicola H Lou W Hou L Salvador RJ Eisenberg GH Cohen 《Canadian Metallurgical Quarterly》1998,72(7):5937-5947
Previously, we showed that truncated soluble forms of herpes simplex virus (HSV) glycoprotein D (gDt) bound directly to a truncated soluble form of the herpesvirus entry mediator (HveAt, formerly HVEMt), a cellular receptor for HSV. The purpose of the present study was to determine the affinity of gDt for HveAt by surface plasmon resonance and to compare and contrast the kinetics of an expanded panel of gDt variants in binding to HveAt in an effort to better understand the mechanism of receptor binding and virus entry. Both HveAt and gDt are dimers in solution and interact with a 2:1 stoichiometry. With HveAt, gD1(306t) (from the KOS strain of HSV-1) had a dissociation constant (KD) of 3.2 x 10(-6) M and gD2(306t) had a KD of 1.5 x 10(-6) M. The interaction between gDt and HveAt fits a 1:1 Langmuir binding model, i.e., two dimers of HveAt may act as one binding unit to interact with one dimer of gDt as the second binding unit. A gD variant lacking all signals for N-linked oligosaccharides had an affinity for HveAt similar to that of gD1(306t). A variant lacking the bond from cysteine 1 to cysteine 5 had an affinity for HveAt that did not differ from that of the wild type. However, variants with double cysteine mutations that eliminated either of the other two disulfide bonds showed decreased affinity for HveAt. This result suggests that two of the three disulfide bonds of gD are important for receptor binding. Four nonfunctional gDt variants, each representing one functional domain of gD, were also studied. Mutations in functional regions I and II drastically decreased the affinity of gDt for HveAt. Surprisingly, a variant with an insertion in functional region III had a wild-type level of affinity for HveAt, suggesting that this domain may function in virus entry at a step other than receptor binding. A variant with a deletion in functional region IV [gD1(Delta290-299t)] exhibited a 100-fold enhancement in affinity for HveAt (KD = 3.3 x 10(-8) M) due mainly to a 40-fold increase in its kinetic on rate. This agrees with the results of other studies showing the enhanced ability of gD1(Delta290-299t) to block infection. Interestingly, all the variants with decreased affinities for HveAt exhibited decreased kinetic on rates but only minor changes in their kinetic off rates. The results suggest that once the complex between gDt and HveAt forms, its stability is unaffected by a variety of changes in gD. 相似文献
64.
G Addolorato E Capristo AV Greco GF Stefanini G Gasbarrini 《Canadian Metallurgical Quarterly》1998,244(5):387-395
OBJECTIVES: To evaluate the influence of chronic alcohol abuse on body composition and energy metabolism in patients affected by chronic alcoholism (group A) compared with a group of healthy social drinkers (group B). SETTING: A university hospital clinic in Italy. SUBJECTS: A total of 32 alcoholics without clinical or laboratory signs of liver cirrhosis and malabsorption. MEASUREMENTS: Body composition was assessed by anthropometric measurements. Resting energy expenditure (REE) and substrate oxidation rate was measured by indirect calorimetry. Daily caloric intake was computed on the basis of a food diary compiled over 7 days. RESULTS: Alcoholics showed a significantly lower body weight (P < 0.05) and a significant lower fat mass (P < 0.05) compared with controls. A higher waist-to-hip ratio was found in group A than in group B, both as a whole group (P < 0.01) or separated by gender (females, P < 0.01) and males, P < 0.001), indicating a prevalence of fat distribution in the abdominal region in alcoholics. REE was significantly higher in group A than in group B (P < 0.05). The non-protein respiratory quotient was significantly lower in group A than in group B (P < 0.001) with a consequent higher utilization of lipids (P < 0.01) and a lower carbohydrate oxidation (P < 0.05) in group A. The energy intake provided only by food ingestion was found to be significantly higher in group B (P < 0.01), whilst the total caloric intake, computed as food intake plus alcohol intake, was higher in group A (P < 0.01). CONCLUSIONS: Alcoholics, as compared with social drinkers, showed a lower body weight due essentially to a fat mass reduction, a higher REE value normalized by fat-free mass, and a preferential utilization of lipids as energy substrate. These findings might suggest that chronic ethanol abuse is able to determine an impairment of nutritional status due, at least in part, to an alteration of the substrate oxidation. 相似文献
65.
JC Jacquier AV Gorelov DM McLoughlin KA Dawson 《Canadian Metallurgical Quarterly》1998,817(1-2):263-271
Due to the growing interest in the use of cationic surfactants for the construction of liposomal genetic delivery systems, the study of complex formation between DNA and quaternary ammonium detergents is of fundamental importance. In this context, we undertook the study of this complex formation using capillary zone electrophoresis (CZE) with suppressed electroosmotic flow, a technique that allowed us to both monitor the change in mobility of DNA as a function of added surfactant in a precise and reproducible manner and evaluate the potential of CZE to reflect the change in hydrodynamic friction upon binding. Nevertheless, CZE must be applied with caution for binding studies where strong cooperativity occurs, because of the presence of peak splitting at concentrations close to the half-point of binding. Also, a comparison between this experiment and Manning's polyelectrolyte transport properties theory on one hand and Tirado and Garcia de la Torre expression for hydrodynamic friction of rod-like molecules on the other hand is given. 相似文献
66.
67.
68.
An algorythm of differential diagnosis conduction of the transplant rejection reaction and the diseases caused by herpes viruses in recipients after the kidney transplantation was elaborated, basing on the analysis of results of the clinical, laboratory, immunological and serological examination methods. 相似文献
69.
70.