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71.
Aikaterini Papakonstantinou Petrina Koumarianou Aimilia Rigakou Panagiotis Diamantakos Efseveia Frakolaki Niki Vassilaki Evangelia Chavdoula Eleni Melliou Prokopios Magiatis Haralabia Boleti 《International journal of molecular sciences》2023,24(1)
Olive oil phenols (OOPs) are associated with the prevention of many human cancers. Some of these have been shown to inhibit cell proliferation and induce apoptosis. However, no systematic comparative study exists for all the investigated compounds under the same conditions, due to difficulties in their isolation or synthesis. Herein are presented innovative methods for large-scale selective extraction of six major secoiridoids from olive oil or leaves enabling their detailed investigation. The cytotoxic/antiproliferative bioactivity of these six compounds was evaluated on sixteen human cancer cell lines originating from eight different tissues. Cell viability with half-maximal effective concentrations (EC50) was evaluated after 72 h treatments. Antiproliferative and pro-apoptotic effects were also assessed for the most bioactive compounds (EC50 ≤ 50 μM). Oleocanthal (1) showed the strongest antiproliferative/cytotoxic activity in most cancer cell lines (EC50: 9–20 μM). The relative effectiveness of the six OOPs was: oleocanthal (1) > oleuropein aglycone (3a,b) > ligstroside aglycone (4a,b) > oleacein (2) > oleomissional (6a,b,c) > oleocanthalic acid (7). This is the first detailed study comparing the bioactivity of six OOPs in such a wide array of cancer cell lines, providing a reference for their relative antiproliferative/cytotoxic effect in the investigated cancers. 相似文献
72.
Georgios Lioulios Zoi Mitsoglou Asimina Fylaktou Aliki Xochelli Michalis Christodoulou Stamatia Stai Eleni Moysidou Afroditi Konstantouli Vasiliki Nikolaidou Aikaterini Papagianni Maria Stangou 《International journal of molecular sciences》2022,23(22)
Lupus nephritis (LN), a chronic inflammatory disease, is characterized by the substantial disruption of immune homeostasis. This study examines its effects on the T lymphocyte phenotype and, particularly, its senescence- and exhaustion-related immune alterations. T cell subpopulations were determined with flow cytometry in 30 LN patients and 20 healthy controls (HCs), according to the expression of senescence- (CD45RA, CCR7, CD31, CD28, CD57), and exhaustion- (PD1) related markers. The immune phenotype was associated with disease activity and renal histology. LN patients were characterized by pronounced lymphopenia, mainly affecting the CD4 compartment, with a concurrent reduction in the naïve, central and effector memory subsets compared to the HCs. In the CD8 compartment, the naïve subsets were significantly lower than that of the HCs, but a shift in the T cells occurred towards the central memory population. CD4+PD1+ and CD8+PD1+ cells were increased in the LN patients compared to the HCs. However, in CD4 T cells, the increase was limited to CD45RA+, whereas in CD8 T cells, both CD45RA+ and CD45RA− subsets were affected. Disease activity was correlated with CD4+PD1+ and highly differentiated CD4+CD28-CD57+ cells. Histology was only associated with CD4 T cell disturbances, with stage IV presenting reduced naïve and increased senescent subsets. Exhausted T lymphocyte subpopulations predominate within LN patients, while the T cell phenotype varies depending on disease activity. 相似文献
73.
Eleni Kalogianni Leonidas Vardakas Aikaterini Vourka Nicholas Koutsikos Christos Theodoropoulos Tomas Galia Nikolaos Skoulikidis 《河流研究与利用》2020,36(5):819-827
Interspecies and intraspecies variation in the use of wooded habitats has been studied for salmonids, but little is known about nonsalmonid species. In this study, we explored spatiotemporal differences in the distribution of three cyprinid species at habitats of varying wood density and at habitats of varying heterogeneity in a Mediterranean intermittent river (Evrotas, southern Greece). Hydroecological data from 843 microhabitats across four sites and in five sampling periods were collected and analysed using generalized linear mixed effects models. Adult chubs, minnowroaches, and minnows and, to a lesser degree, juvenile chubs, preferred complex, wooded habitats, characterized by lower flow velocities, higher water depths, finer substrate, and increased canopy cover, compared with wood‐free and noncomplex habitats. At the advanced stage of the dry period, the deeper, wooded habitats served as drought refugia; all fish groups, except for the adult chub, resorted to these habitats during autumn. In contrast, no such shifts were evident in early summer at the onset of the dry period. In late spring, all fish groups had higher densities at wooded habitats, a shift probably associated with reproduction. Wood density did not affect fish habitat use, as fish densities were similar across wooded habitats. In contrast, habitat heterogeneity significantly affected fish densities; more adult chubs and minnows were observed in complex habitats, whereas juvenile chubs and minnowroaches clearly preferred less complex habitats. We conclude that the targeted maintenance of instream wood structures to ensure habitat heterogeneity is a key‐action for the successful conservation of riverine fish populations. 相似文献
74.
Aikaterini Argyrou 《国际水》2019,44(6-7):752-768
ABSTRACTLegislation in New Zealand dictates that the Whanganui River is a living entity and a legal person. Guardians uphold the river’s environmental, social, cultural and economic well-being. We provide a conceptual discussion of the river’s economic well-being, understood as the mutual enhancement of natural and human elements through community entrepreneurship that is based on human and non-human capabilities. We discuss human economic activity that preserves the right of the river to be free from pollution and form an integral part of the Māori culture and tradition, the improvement of Māori living conditions, and their rights to self-determination and prior consent. 相似文献
75.
Aikaterini Pistiki Anuradha Ramoji Oleg Ryabchykov Daniel Thomas-Rüddel Adrian T. Press Oliwia Makarewicz Evangelos J. Giamarellos-Bourboulis Michael Bauer Thomas Bocklitz Jürgen Popp Ute Neugebauer 《International journal of molecular sciences》2021,22(19)
Biochemical information from activated leukocytes provide valuable diagnostic information. In this study, Raman spectroscopy was applied as a label-free analytical technique to characterize the activation pattern of leukocyte subpopulations in an in vitro infection model. Neutrophils, monocytes, and lymphocytes were isolated from healthy volunteers and stimulated with heat-inactivated clinical isolates of Candida albicans, Staphylococcus aureus, and Klebsiella pneumoniae. Binary classification models could identify the presence of infection for monocytes and lymphocytes, classify the type of infection as bacterial or fungal for neutrophils, monocytes, and lymphocytes and distinguish the cause of infection as Gram-negative or Gram-positive bacteria in the monocyte subpopulation. Changes in single-cell Raman spectra, upon leukocyte stimulation, can be explained with biochemical changes due to the leukocyte’s specific reaction to each type of pathogen. Raman spectra of leukocytes from the in vitro infection model were compared with spectra from leukocytes of patients with infection (DRKS-ID: DRKS00006265) with the same pathogen groups, and a good agreement was revealed. Our study elucidates the potential of Raman spectroscopy-based single-cell analysis for the differentiation of circulating leukocyte subtypes and identification of the infection by probing the molecular phenotype of those cells. 相似文献
76.
Aikaterini Papazi Armida-Irene GjindaliElizabeth Kastanaki Konstantinos AssimakopoulosKonstantinos Stamatakis Kiriakos Kotzabasis 《International Journal of Hydrogen Energy》2014
Hydrogen is considered to be the future optimal energy carrier, and is expected to contribute to the growth of the world's economy by facilitating a stable supply of energy. The ability of green algae to produce hydrogen was discovered 74 years ago. Since then, several attempts were made, to increase hydrogen production yields, sulfur starvation being the best known. The main concern during these attempts was that the achievable increase in yield was not sustainable. In this contribution, potassium deficiency is presented as a biochemical/bioenergetic switch for a sustained high yield of hydrogen production via the photosynthetic apparatus. Potassium can partially be replaced by sodium in the majority of biochemical processes and as a result the system remains functional. However, sodium cannot replace potassium in the conversion of glucose to starch. This fact significantly increased the yield of hydrogen production through the Photosystem II independent pathway, since electrons originating from the metabolism of glucose are used in the continuous donation to the plastoquinone-pool of the photosynthetic electron chain. Additionally, PSII inactivation (and therefore the inhibition of O2-production), the further synthesis and over activation of Photosystem I and plastidic hydrogenase, generated a sustained increase in hydrogen production, mainly through the PSII-independent pathway. The self regulation of these multistage processes in hermitically closed static systems of Scenedesmus obliquus cultivation, permitted the establishment of anoxic conditions and the continuous electron supply to highly activated hydrogenase, resulting in the sustained high yield hydrogen production and paving the way for future usage in an industrial scale application. 相似文献
77.
Ioannis Tsialtas Achilleas Georgantopoulos Maria E. Karipidou Foteini D. Kalousi Aikaterini G. Karra Demetrios D. Leonidas Anna-Maria G. Psarra 《International journal of molecular sciences》2021,22(14)
Estrogens are steroid hormones that play a crucial role in the regulation of the reproductive and non-reproductive system physiology. Among non-reproductive systems, the nervous system is mainly affected by estrogens due to their antioxidant, anti-apoptotic, and anti-inflammatory activities, which are mediated by membranous and nuclear estrogen receptors, and also by non-estrogen receptor-associated estrogen actions. Neuronal viability and functionality are also associated with the maintenance of mitochondrial functions. Recently, the localization of estrogen receptors, especially estrogen receptor beta, in the mitochondria of many types of neuronal cells is documented, indicating the direct involvement of the mitochondrial estrogen receptor beta (mtERβ) in the maintenance of neuronal physiology. In this study, cell lines of N2A cells stably overexpressing a mitochondrial-targeted estrogen receptor beta were generated and further analyzed to study the direct involvement of mtERβ in estrogen neuroprotective antioxidant and anti-apoptotic actions. Results from this study revealed that the presence of estrogen receptor beta in mitochondria render N2A cells more resistant to staurosporine- and H2O2-induced apoptotic stimuli, as indicated by the reduced activation of caspase-9 and -3, the increased cell viability, the increased ATP production, and the increased resistance to mitochondrial impairment in the presence or absence of 17-β estradiol (E2). Thus, the direct involvement of mtERβ in antioxidant and anti-apoptotic activities is documented, rendering mtERβ a promising therapeutic target for mitochondrial dysfunction-associated degenerative diseases. 相似文献
78.
Andrea Coppola Barbara Capuani Francesca Pacifici Donatella Pastore Roberto Arriga Alfonso Bellia Aikaterini Andreadi Nicola Di Daniele Renato Lauro David Della-Morte Giuseppe Sconocchia Davide Lauro 《International journal of molecular sciences》2021,22(15)
Activation of innate immunity and low-grade inflammation contributes to hyperglycemia and an onset of Type 2 Diabetes Mellitus (T2DM). Interleukin-2 (IL-2), leptin, High Mobility Group Box-1 (HMGB-1), and increased glucose concentrations are mediators of these processes also by modulating peripheral blood mononuclear cells (PBMCs) response. The aim of this study was to investigate if HMGB-1 and IL-2 turn on PBMCs and their leptin secretion. In isolated human PBMCs and their subpopulations from healthy individuals and naïve T2DM patients, leptin release, pro-inflammatory response and Toll-like Receptors (TLRs) activation was measured. After treatment with IL-2 and HMGB1, NK (Natural Killer) have the highest amount of leptin secretion, whilst NK-T have the maximal release in basal conditions. TLR4 (TAK242) and/or TLR2 (TLR2-IgA) inhibitors decreased leptin secretion after IL-2 and HMGB1 treatment. A further non-significant increase in leptin secretion was reported in PBMCs of naive T2DM patients in response to IL-2 and HMGB-1 stimulation. Finally, hyperglycemia or hyperinsulinemia might stimulate leptin secretion from PBMCs. The amount of leptin released from PBMCs after the different treatments was enough to stimulate the secretion of IL-1β from monocytes. Targeting leptin sera levels and secretion from PBMCs could represent a new therapeutic strategy to counteract metabolic diseases such as T2DM. 相似文献
79.
80.
Columns of Greek Temples under seismic loading: Columns of ancient Greek Temples consist of accurately dressed individual blocks in total absence on binding mortars. The seismic response of such multi‐drum structures is completely different than the corresponding one of modern continuous structures. Their stability is governed by independent sliding and rocking of drums, which creates an external energy absorption mechanism. This paper presents numerical studies on the dynamic behaviour of ancient columns which exhibits a highly non‐linear response. Alternative modeling methods are used and calibrated to experimental results. It is concluded that ancient columns may be less vulnerable to seismic events compared to modern structures. The perfection of the form unified esthetical, architectural and structural requirements. 相似文献