Phage‐mediated transfer of virulence genes

Bacteriophages as accessory genetic elements play a crucial role in the dissemination of genes and the promotion of genetic diversity within bacterial populations. Such horizontal transfer of DNA is critical in the emergence of new pathogenic organisms, through the dissemination of genes encoding virulence factors such as toxins, adhesins and agressins. Phages can transfer genes that are not necessary for bacteriophage persistence and are generally recognised by their ability to convert their host bacteria to new phenotypes. This phenomenon is known as phage conversion. If such converting genes encode for virulence factors, the consequences of phage infection may include increased virulence of the host bacteria, and the conversion of a non‐pathogenic strain to a potentially dangerous pathogen. A number of virulence factors in bacteria causing diseases in plants, animals and humans are encoded by converting phages, the vast majority of which are temperate as opposed to lytic in nature. © 2001 Society of Chemical Industry     

The Dramatic Impact of Silicon Technology

Approximately twelve years ago the first double-diffused silicon transistor was offered to the marketplace. These first transistors sold for $70.00. Today it is possible to purchase an integrated memory circuit with several thousand transistors plus several thousand resistors and diodes for less the original selling price of this first transistor.     

Biomass Recovery Method for Adenosine Triphosphate (ATP) Quantification Following UV Disinfection

A biomass recovery method was developed to monitor UV disinfection efficacy using adenosine triphosphate (ATP). Typically, disinfection monitoring at wastewater treatment facilities (WWTFs) involves quantifying fecal and total coliforms or colony forming units, the results of which take a minimum of 24 h to produce. ATP quantification immediately before and after UV treatment, which takes only minutes, shows little reduction and often an increase in the microbial population since UV irradiation results in cells that are viable (i.e., still producing ATP) but not culturable. To overcome this, our biomass recovery method incorporates an incubation step to encourage life cycling of microbes. Average log reductions in cellular ATP (cATP) were found to be ?0.28 ± 0.19, ?0.011 ± 0.153, ?0.17 ± 0.32, and 0.065 ± 0.074 using direct ATP measurements on UV-treated samples from WWTFs A, B, C, and D, respectively, while those using the recovery method were correspondingly 0.17 ± 0.34, 1.8 ± 0.8, 0.20 ± 0.35, and 0.72 ± 0.26. The response of the biomass recovery-ATP method indicated a significant direct correlation to the microbial population reduction observed in heterotrophic plate count (HPC) and Colilert® methods using both pure Escherichia coli culture and secondary municipal wastewater effluent.     

The integration of a satellite spectral analysis into a heather moorland management model (HMMM): The case of Moidach More,northeast Scotland,U.K.

In this paper, high resolution, remotely-sensed data are investigated as a source of management information for moorland vegetation communities on a hypothetical farm in a marginal farming area of Scotland. A hybrid technique for vegetation mapping is adopted. This involves unsupervised machine classification of spectral variation in digitally enhanced air photographs and satellite imagery, together with visual interpretation of the imagery in both the laboratory and the field. This technique enables relative estimates of annual gross biomass production, for a range of vegetation types, to be obtained. The data are integrated into a simple heather moorland management model (HMMM), to produce potential sheep-stocking capacities under a range of management scenarios, the possible financial implications of these scenarios are also assessed.     

Characteristics of college cyberbullies

Cyberbullying is a newer variation of bullying that utilizes technology to harass. This study investigated the psychological symptomology, suicidal behaviors, aggressive tendencies, and illegal behaviors of college cyberbullies. Sixty cyberbullies and 19 cyberbully/victims (participants who cyberbullied and were victims of cyberbullying) scored higher in psychological symptoms of depression, hostility, interpersonal sensitivity, paranoia, phobic anxiety, and psychoticism than participants who were uninvolved with cyberbullying. The cyberbullies also endorsed more suicidal behaviors and scored higher in aggression. The cyberbully/victims engaged in more violent and drug crimes than cyberbullies and controls. These findings indicate perpetrators of cyberbullying have more psychological distress, aggressive tendencies, and engage in more illegal behaviors than those who do not cyberbully. This is the first study to show the distress cyberbullies are experiencing.     

外包在中国

目前，中国正逐渐成为西方寻找外包开发游戏公司的一片新热土，Pearl Research认为中国市场上存在的大量劳动力，相对较低的劳务成本，大批的游戏玩家以及完善的设施都是形成这种态势的重要原因。通过向中国开发公司外包，仅就游戏美工一项，往往就可以帮助西方公司节约20%以上的开发成本，在次时代游戏开发成本超越400万美元的今天，开发成本的减少将直接提升公司的毛利率，并对游戏产品的获利与否产生直接影响。