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41.
Régis Riveret Pietro Baroni Yang Gao Guido Governatori Antonino Rotolo Giovanni Sartor 《Annals of Mathematics and Artificial Intelligence》2018,83(1):21-71
The combination of argumentation and probability paves the way to new accounts of qualitative and quantitative uncertainty, thereby offering new theoretical and applicative opportunities. Due to a variety of interests, probabilistic argumentation is approached in the literature with different frameworks, pertaining to structured and abstract argumentation, and with respect to diverse types of uncertainty, in particular the uncertainty on the credibility of the premises, the uncertainty about which arguments to consider, and the uncertainty on the acceptance status of arguments or statements. Towards a general framework for probabilistic argumentation, we investigate a labelling-oriented framework encompassing a basic setting for rule-based argumentation and its (semi-) abstract account, along with diverse types of uncertainty. Our framework provides a systematic treatment of various kinds of uncertainty and of their relationships and allows us to back or question assertions from the literature. 相似文献
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Amedeo Biasi Valerio Marino Giuditta Dal Cortivo Paolo Enrico Maltese Antonio Mattia Modarelli Matteo Bertelli Leonardo Colombo Daniele DellOrco 《International journal of molecular sciences》2021,22(19)
Guanylate cyclase-activating protein 1 (GCAP1), encoded by the GUCA1A gene, is a neuronal calcium sensor protein involved in shaping the photoresponse kinetics in cones and rods. GCAP1 accelerates or slows the cGMP synthesis operated by retinal guanylate cyclase (GC) based on the light-dependent levels of intracellular Ca2+, thereby ensuring a timely regulation of the phototransduction cascade. We found a novel variant of GUCA1A in a patient affected by autosomal dominant cone dystrophy (adCOD), leading to the Asn104His (N104H) amino acid substitution at the protein level. While biochemical analysis of the recombinant protein showed impaired Ca2+ sensitivity of the variant, structural properties investigated by circular dichroism and limited proteolysis excluded major structural rearrangements induced by the mutation. Analytical gel filtration profiles and dynamic light scattering were compatible with a dimeric protein both in the presence of Mg2+ alone and Mg2+ and Ca2+. Enzymatic assays showed that N104H-GCAP1 strongly interacts with the GC, with an affinity that doubles that of the WT. The doubled IC50 value of the novel variant (520 nM for N104H vs. 260 nM for the WT) is compatible with a constitutive activity of GC at physiological levels of Ca2+. The structural region at the interface with the GC may acquire enhanced flexibility under high Ca2+ conditions, as suggested by 2 μs molecular dynamics simulations. The altered interaction with GC would cause hyper-activity of the enzyme at both low and high Ca2+ levels, which would ultimately lead to toxic accumulation of cGMP and Ca2+ in the photoreceptor outer segment, thus triggering cell death. 相似文献
44.
Giuseppe Gullo Andrea Etrusco Gaspare Cucinella Antonino Perino Vito Chiantera Antonio Simone Lagan Rossella Tomaiuolo Amerigo Vitagliano Pierluigi Giampaolino Marco Noventa Alessandra Andrisani Giovanni Buzzaccarini 《International journal of molecular sciences》2021,22(21)
Endometrial cancer (EC) is a deleterious condition which strongly affects a woman’s quality of life. Although aggressive interventions should be considered to treat high-grade EC, a conservative approach should be taken into consideration for women wishing to conceive. In this scenario, we present an overview about the EC fertility-sparing approach state of art. Type I EC at low stage is the only histological type which can be addressed with a fertility-sparing approach. Moreover, no myometrium and/or adnexal invasion should be seen, and lymph-vascular space should not be involved. Regarding the pharmaceutical target, progestins, in particular medroxyprogesterone acetate (MPA) or megestrol acetate (MA), are the most employed agent in conservative treatment of early-stage EC. The metformin usage and hysteroscopic assessment is still under debate, despite promising results. Particularly strict and imperious attention should be given to the follow-up and psychological wellbeing of women, especially because of the double detrimental impairment: both EC and EC-related infertility consequences. 相似文献
45.
Antonino Giaccone Giovanni Lascari Giorgia Peri Gianfranco Rizzo 《Energy Efficiency》2017,10(1):129-149
Energy master plans are important tools for an effective and sustainable land governance. On the other hand, the stakeholder role in setting priorities for planning energy interventions is dramatically increasing, also in the light of recently issued European directives. The priorities of energy-saving measures of the Sicilian energy and environmental master plan were originally established with the application of typical economic indicators (the cost of saved energy and the cost of avoided pollutant emissions). During the prioritization process, there was a minor contribution from stakeholders who were not directly involved in the priority-setting process but were only asked to express their opinions according to the in-force regulations. Based on more active stakeholder involvement in hierarchizing a given set of actions, as required by the recently issued directives, the University of Palermo, which participated in the development of this master plan, assessed an ex post evaluation of these established economy-based priorities, by means of the application of a multi-criteria decision analysis (MCDA) tool. The resulting changed priorities, which lead to a better policy allocation of the regional budget for energy efficiency in the building sector, confirm the preeminent role played by the stakeholders in the development of energy plans, further supported by a sensitivity analysis. 相似文献
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47.
Monica Virginia Gianfranceschi Maria Claudia D'Ottavio Antonietta Gattuso Antonino Bella Paolo Aureli 《Food microbiology》2009
This work was undertaken to study the serotypes and pulsotypes of 674 Listeria monocytogenes isolates from human (57), food (558) and environmental (59) sources, collected from different Italian geographical areas during 2002–2005, to determine whether certain subtypes were associated with certain foods and more often involved in cases of listeriosis, and to determine possible geographical or temporal associations. Eleven different L. monocytogenes serotypes were found in the food, environmental and human isolates. Most isolates belonged to only four serotypes (1/2a, 1/2b, 1/2c, 4b). The isolates were divided into 133 distinct AscI pulsotypes grouped into 26 pulsogroups. Pulsogroups ranged from a minimum of 2 up to 212 isolates, and contained 1–19 different pulsotypes. When associations between subtypes and isolates from specific foods selected as being most frequently involved in cases of listeriosis were tested some of these associations were highly significant but not exclusive, indicating that there was no close correlation between specific subtypes and specific food products. Despite the limitations of this study (few human isolates versus many food isolates prevalently collected from one food category), we believe that a large-scale database of L. monocytogenes subtypes and a timely epidemiological investigation can facilitate risk assessment and outbreak detection and control. 相似文献
48.
Federica Maltese Cornelis Erkelens Frank van der Kooy Young Hae Choi Robert Verpoorte 《Food chemistry》2009
Recently advanced analytical technology has provided evidence of the existence of stereoisomers of many natural products. Particularly, flavanones which might have two different configurations at C-2 exist in many food additives, e.g., citrus fruits. In this study, the possible stereoisomers of flavanone glycosides were identified by NMR spectroscopy. Based on NMR spectra of common flavanone glycosides such as naringin, hesperidin, and neohesperidin, the two existing diastereomeric forms of the molecules could clearly be distinguished. The 1H NMR resonances of two diastereomers of each flavanone glycosides investigated in this study were fully assigned with the assistance of diverse 2D NMR spectroscopy methods. 相似文献
49.
Nunzia DOnofrio Elisa Martino Luigi Mele Antonino Colloca Martina Maione Domenico Cautela Domenico Castaldo Maria Luisa Balestrieri 《International journal of molecular sciences》2021,22(15)
Understanding the mechanisms of colorectal cancer progression is crucial in the setting of strategies for its prevention. δ-Valerobetaine (δVB) is an emerging dietary metabolite showing cytotoxic activity in colon cancer cells via autophagy and apoptosis. Here, we aimed to deepen current knowledge on the mechanism of δVB-induced colon cancer cell death by investigating the apoptotic cascade in colorectal adenocarcinoma SW480 and SW620 cells and evaluating the molecular players of mitochondrial dysfunction. Results indicated that δVB reduced cell viability in a time-dependent manner, reaching IC50 after 72 h of incubation with δVB 1.5 mM, and caused a G2/M cell cycle arrest with upregulation of cyclin A and cyclin B protein levels. The increased apoptotic cell rate occurred via caspase-3 activation with a concomitant loss in mitochondrial membrane potential and SIRT3 downregulation. Functional studies indicated that δVB activated mitochondrial apoptosis through PINK1/Parkin pathways, as upregulation of PINK1, Parkin, and LC3B protein levels was observed (p < 0.0001). Together, these findings support a critical role of PINK1/Parkin-mediated mitophagy in mitochondrial dysfunction and apoptosis induced by δVB in SW480 and SW620 colon cancer cells. 相似文献
50.
Agnese Filippello Stefania Di Mauro Alessandra Scamporrino Roberta Malaguarnera Sebastiano Alfio Torrisi Gian Marco Leggio Antonino Di Pino Roberto Scicali Francesco Purrello Salvatore Piro 《International journal of molecular sciences》2021,22(13)
Intestinal organoids are used to analyze the differentiation of enteroendocrine cells (EECs) and to manipulate their density for treating type 2 diabetes. EEC differentiation is a continuous process tightly regulated in the gut by a complex regulatory network. However, the effect of chronic hyperglycemia, in the modulation of regulatory networks controlling identity and differentiation of EECs, has not been analyzed. This study aimed to investigate the effect of glucotoxicity on EEC differentiation in small intestinal organoid platforms. Mouse intestinal organoids were cultured in the presence/absence of high glucose concentrations (35 mM) for 48 h to mimic glucotoxicity. Chronic hyperglycemia impaired the expression of markers related to the differentiation of EEC progenitors (Ngn3) and L-cells (NeuroD1), and it also reduced the expression of Gcg and GLP-1 positive cell number. In addition, the expression of intestinal stem cell markers was reduced in organoids exposed to high glucose concentrations. Our data indicate that glucotoxicity impairs L-cell differentiation, which could be associated with decreased intestinal stem cell proliferative capacity. This study provides the identification of new targets involved in new molecular signaling mechanisms impaired by glucotoxicity that could be a useful tool for the treatment of type 2 diabetes. 相似文献