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891.
892.
The binding of 125I-labelled tissue plasminogen activator (tPA), the tPA A- or B-chain to endothelial cells (EC) were studied in suspensions of cultured human umbilical vein EC (HUVEC) or immortalized microvascular EC (HMEC). By determinations of the concentration-dependent binding it was shown that both the A-chain and the B-chain, which were isolated after partial reduction of two-chain tPA, contain ligands for binding to EC. The affinity for the B-chain was much higher than for the A-chain according to Scatchard analysis (Kd 24 and 515 nM, respectively), whereas the number of binding sites was higher for the A-chain than for the B-chain (Bmax 8 x 10(5) and 1.2 x 10(5), respectively). There were no cross interactions between the A- and B-chains and their binding sites. The binding of tPA to EC induced an almost 100-fold increase of the activation rate when compared to the same amount of enzyme in free solution, which in contrast to the fibrin-induced stimulation was not inhibited by antibodies against fibrin. The enzymatic activity of the B-chain was much less affected by the association to the cells. Both tPA and the tPA B-chain were largely protected against inhibition by an excess plasminogen activator type-1 (PAI-1) when bound to EC, whereas the same amount of free tPA was totally inactivated. The competition studies strongly indicated that an N-terminal segment in the B-chain, AKHRRSPGER, may be the ligand part of the B-chain. It is interesting to note that this polypeptide segment also participates in a binding site for PAI-1, necessary for effective inhibition. This implies a possible competition between PAI-1 and a tPA-receptor for binding of tPA. High molecular weight urokinase had no quenching effect on the binding of the B-chain to EC. 相似文献
893.
The authors report on a flexible system that provides visual feedback to VLSI designers with a novel display method. The basic problem is to display simultaneously two or more functions f 1, f 2, . . ., each of which depends on the two spatial variables x and y . The method is based on results from visual perception experiments, which indicated that the human visual system can view simultaneously two or more images that are separated in depth, even if they are combined (superimposed) over a common spatial domain. Two display modes have been implemented: a gray-level mode and a contour mode in which only the edges separating adjacent regions are displayed in an effort to make things easier for the user. This depth-separation technique allows the viewer to register spatially the x ,y distribution of multiple variables in an animation sequence. The technique can be applied to many other situations in which a single-plane episode involves several variables 相似文献
894.
A. S. Anishchenko Yu. V. Feofanov A. B. Bogun 《Chemical and Petroleum Engineering》1992,28(11):693-695
Translated from Khimicheskoe i Neftyanoe Mashinostroenie, No. 11, pp. 33–35, November, 1992. 相似文献
895.
Steimann F. Hayde M. Panzenbock B. Adlassnig K.-P. Pollak A. 《IEEE engineering in medicine and biology magazine》1994,13(5):705-709
ONSET derives its diagnosis from the combined evidence of contemporaneous and successive findings, which is in accordance with the behaviour of a clinician. Although it does not increase the diagnostic capabilities in principle, i.e., it cannot perform better than an expert reasoning on the basis of the same knowledge, computational power can be exploited to perform the matches and combine the results with greater precision, resolution, reliability, and transparency. Fundamental improvements of diagnosis, however, can only be expected from better serological tests or increased testing frequency. ONSET relies on domain knowledge explicated in a non-verbalized, analytical form. Knowledge representation-although mathematical-is intuitively clear and easy to conceive, making it equally accessible to both man and machine, and hence subject to objective evaluation and criticism 相似文献
896.
R. B. Buitendijk 《Computational Economics》1988,1(2):79-96
SQL is often used to express questions imposed by users. It is well known that this process is not error-free in that it may result in syntactically or semantically incorrect SQL queries. This article is meant to probe deeper into SQL queries that are incorrect, due to delusion errors. A classification of natural language questions will be introduced. The purpose of this classification is two-fold. Firstly, it gives insight into possible errors and enables us to distinguish between types of errors. Secondly, it is a means of determining the influence of measures (for instance, SQL training) that can be taken to reduce the number of aforementioned errors. For discussion, several of these measures will be presented and related to the classification.Abbreviations RA
Relational algebra
- SQL
structured query language 相似文献
897.
898.
Benson Janette B.; Cherny S. S.; Haith Marshall M.; Fulker David W. 《Canadian Metallurgical Quarterly》1993,29(3):434
Assessed infant predictors of adult IQ with same-sex infant twins (114 pairs) and their parents. The midtwin–midparent design permits the rapid assessment of infant measures to predict later behavior, because the midparent score serves as a proxy for the infant's potential score at maturity. At 5, 7, and 9 mo, Ss were observed on the Fagan Test of Infant Intelligence, hand preference, vocalizations, selected Bayley Scales of Infant Development items, and a modified Bayley Infant Behavior Record. At 8 mo, Ss received the Visual Expectation Paradigm and an auditory discrimination task. Their parents received the Wechsler Adult Intelligence Scale—Revised (WAIS—R). Some infant measures, indicative of information processing, language ability, and temperament, predicted midparent IQ. This study extended and partially replicated findings from a previous midtwin–midparent cohort (L. F. DiLalla et al; see record 1991-04066-001). (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
899.
900.
Illustrates how discrete-time survival analysis can address questions about onset, cessation, relapse, and recovery. Using data on the onset of suicide ideation and depression and relapse into cocaine use, the authors introduce key concepts underpinning the method, describe the action of the discrete-time hazard model, and discuss several types of main effects and interactions that can be included as predictors. Practical issues of data analysis and strategies for interpretation and presentation are discussed. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献