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61.
62.
Ionizing radiation is believed to stimulate the repopulation of squamous carcinoma cells that survive the early portion of a fractionated course of radiotherapy. To characterize any intrinsic radiation-induced adaptive response and to examine whether epidermal growth factor (EGF) influences this response, A431 and 183A cells were irradiated with repeated daily exposures of 0.5-0.75 Gy and then grown in monolayer culture for 7 days with or without EGF at a 1 ng/ml concentration. Cell numbers were quantified using a microtiter dye-reduction assay. EGF alone caused approximately 70% and 30% growth inhibition of human SC A431 and 183A cells, respectively. Although radiation alone did not affect proliferative rates in these conditions, radiation eliminated the EGF-related growth inhibition in both cell lines. This effect was dose dependent in single radiation exposure experiments. Cell cycle analyses indicated that EGF initially promoted entry into S-phase 3 days after treatment but caused a G1-S block after 7 days. Treatment with radiation recruited cells into S-phase and G2-M, an effect which was sustained 7 days after treatment, overriding the influence of EGF. Radiation-induced modulation of the response of human squamous carcinoma cells to EGF in vitro after single and repeated radiation exposures suggests a proliferation response that may underlie enhanced repopulation of tumor clonogens in vivo. 相似文献
63.
Secretory proteins are cotranslationally translocated across the mammalian ER membrane through an aqueous pore in the translocon while the permeability barrier is maintained by a tight ribosome-membrane junction. The lumenal end of the pore is also blocked early in translocation. Extraction of soluble lumenal proteins from microsomes and reconstitution with purified proteins demonstrate, by fluorescence collisional quenching, that BiP seals the lumenal end of this pore. BiP also seals translocons that are assembled but are not engaged in translocation. These ribosome-free translocons have smaller pores (9-15 A diameter versus 40-60 A in functioning translocons) and are generated when ribosomes dissociate from functioning translocons with large pores. BiP therefore maintains the permeability barrier by sealing both nontranslocating and newly targeted translocons. 相似文献
64.
B Susskind RH Kerman R Nelson J Gregory T La A Bayat M Dang C Van Buren S Katz BD Kahan 《Canadian Metallurgical Quarterly》1998,66(12):1823-1826
BACKGROUND: Disadvantages inherent to complement-dependent cytotoxicity cross-match (CDC XM) methods are the requirements for complement and viable target cells, detection of antibodies (Abs) against non-HLA antigens, and subjective scoring. Cross-Stat (SangStat Medical Corp., Menlo Park, CA), a recently developed enzyme-linked immunosorbent assay XM procedure for the detection of IgG anti-donor HLA Abs, is theoretically devoid of these flaws. METHODS: We compared results of Cross-Stat and our standard anti-human globulin (AHG)-enhanced CDC XM procedure on 524 sera from 230 transplant candidates, which were evaluated against 51 cadaveric donors. RESULTS: There was a significant correlation between AHG-CDC IgG XM and Cross-Stat results (P<0.001). For false negative sera, repeat AHG-CDC IgG XMs were still positive after platelet absorption, indicating that the Abs present were either non-HLA Abs or anti-HLA class II. Flow cytometry testing of false positive sera usually (42/62) substantiated Cross-Stat results, indicating that the discrepancy with AHG-CDC IgG XM is caused by greater sensitivity of Cross-Stat. Relative to the AHG-CDC XM, the sensitivity of Cross-Stat was 100%, the specificity was 93%, the positive predictive value was 73%, and the negative predictive value was 100%. A technical shortcoming of the Cross-Stat assay is that the frequency of indeterminate samples in the assays was 15%. Among 49 Cross-Stat negative vs. 13 Cross-Stat positive primary cadaveric renal allograft recipients (all AHG-CDC IgG-XM negative), there was no statistical difference in overall graft survival. CONCLUSION: Given the important theoretical advantages of enzyme-linked immunosorbent assay-based XM methods over the CDC XM, however, further testing of the clinical relevance of the Cross-Stat is warranted. 相似文献
65.
This article examines the attaching and detaching experience of a mother encountering the perinatal death of a twin. Her experience is related to the relevant theoretical and research literature pertaining to prebirth and postbirth maternal-infant attachment and detachment (grieving). Literature for both single infants and twins is considered. The experience of this mother suggests that elements of postbirth attachment may have been accelerated into the prebirth period. In addition, her postbirth attaching and detaching experience suggests that an attachment and detachment to the twins as a unit preceded detachment from the twin who died. The health care provider's role in promoting maternal well-being, and indirectly the well-being of the surviving infant, is described. 相似文献
66.
CVD growth conditions, particularly growth temperature and partial pressures of the reactant gases, strongly affect the growth
characteristics and properties of GaAs0.6P0.4 epitaxial layers grown on GaAs substrates. For LED’s the most important properties of the material are B/J (brightness per
unit current density) and surface morphology. This paper presents the results of a systematic study of the effect of temperature
and reactant gas partial pressure (at a fixed III/V ratio) on B/J, surface morphology, growth rate, impurity doping and layer
composition. Growth conditions which yield the optimum properties for LED’s are determined. The results are interpreted on
the basis of kinetic and thermodynamic mechanisms controlling the growth process under various growth conditions. At constant
temperature and constant III/V ratio, increasing the partial pressures causes the growth process to change from mass transport
limited, where the growth rate increases with increasing partial pressures, to kinetically limited, where the growth rate
is independent of partial pressures. Good morphology layers are obtained over a range of partial pressures around the transition
from mass transport limited to kinetically limited growth. The B/J peaks at a value of partial pressure in the kinetically
limited regime at which good morphology layers are obtained. Although B/J increases with increasing growth rate in the mass
transport regime, the maximum B/J occurs in the region where growth rate is independent of partial pressures so that growth
rate alone is not sufficient to determine B/J. In contrast to the “parabolic≓ dependence of growth rate on growth temperature,
caused by the transition from the mass transport regime to the kinetic regime, the relative incorporation of As, P, and Te
varies with temperature in the manner predicted from thermodynamics in both regimes. This behavior is consistent with the
growth rate in the kinetic regime being limited by the desorption of chlorine atoms from the growth surface, with the reaction
of As, P, and Te with the Ga proceeding thermodynamically at all temperatures. 相似文献
67.
A simple derivation is given for the attenuation coefficient of leaky rays in a graded fibre of circular cross-section, requiring only geometric-optics and plane-wave concepts in a 2-dimensional medium. 相似文献
68.
69.
The performance in estimating a Wiener process that modulates the phase of a subcarrier in a direct-detection optical communication system is studied. An approximation is derived for the steady-state probability density of the estimation error. The meansquare estimation error predicted using this density is compared to theoretical lower bounds on the error and to the sample meansquare error obtained by simulation. 相似文献
70.
Study of the activities of hyaluronidase, lactate dehydrogenase isoenzyme-X and the dehydrogenases of sorbitol, alpha-glycerophosphate, glucose-6-phosphate, malate, glyceraldehyde-3-phosphate and isocitrate in the testes of mice of different ages showed that the changes were correlated with the stages of spermatogenic cell differentiation. The first 4 enzymes had activities which were low in newborn mice and high in adults; this pattern was reversed for the other enzymes. 相似文献