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排序方式： 共有2246条查询结果，搜索用时 15 毫秒
51.
52.
Daniel Padro Philipp Cienskowski Sonia Lopez‐Fernandez Indranath Chakraborty Carolina Carrillo‐Carrion Neus Feliu Wolfgang J. Parak Monica Carril 《Small (Weinheim an der Bergstrasse, Germany)》2020,16(36)
Protein corona formation on the surface of nanoparticles (NPs) is observed in situ by measuring diffusion coefficients of the NPs under the presence of proteins with a 19F nuclear magnetic resonance (NMR) based methodology. Formation of a protein corona reduces the diffusion coefficient of the NPs, based on an increase in their effective hydrodynamic radii. With this methodology it is demonstrated that the apparent dissociation constant of protein–NP complexes may vary over at least nine orders of magnitude for different types of proteins, in line with the Vroman effect. Using this methodology, the interaction between one type of protein and one type of nanoparticle can be studied quantitatively. Due to the NMR‐based detection, this methodology has no interference by absorption/scattering effects, by which optical detection schemes are affected. By using the potential of the NMR chemical shift, the detection of multiple 19F signals simultaneously opens the possibility to study the diffusion of several NPs at the same time. The 19F labeling of the NPs has negligible effect on their acute toxicity and moderate effect on NPs uptake by cells. 相似文献
53.
Manipulating microorganisms with inherent motility is a challenging yet significant aim with implications in many biological, environmental, and technological applications. Many microorganisms that are broadly available in nature can be used as self‐powered systems that can be directed with external stimuli. Paramecium is a unicellular protozoan that exhibits a negative galvanotaxis where the cell follows the direction of weak electric fields. Here, the galvanotactic behavior of Paramecia is studied to achieve the precise manipulation of these organisms. Using a specially devised microfluidic chip and computer vision, unprecedented levels of manipulation and isolation of Paramecia are demonstrated, enabling their integration, use, and study in micro‐electromechanical systems. 相似文献
54.
Phytohormone ethylene regulates numerous aspects of plant physiology, from fruit ripening to pathogen responses. The molecular basis of ethylene biosynthesis and action has been investigated for over 40 years, and a combination of biochemistry, genetics, cell, and molecular biology have proven successful at uncovering the core machinery of the ethylene pathway. A number of molecular tools have been developed over the years that enable visualization of the sites of ethylene production and response in the plant. Genetically encoded biosensors take advantage of reporter proteins, i.e., fluorescent, luminescent, or colorimetric markers, to highlight the tissues that make, perceive, or respond to the hormone. This review describes the different types of biosensors currently available to the ethylene community and discusses potential new strategies for developing the next generation of genetically encoded ethylene reporters. 相似文献
55.
Eduardo Lupi Anirban Ghosh Sahar Saremi Shang‐Lin Hsu Shishir Pandya Gabriel Velarde Abel Fernandez Ramamoorthy Ramesh Lane W. Martin 《Advanced Electronic Materials》2020,6(3)
The ability to produce atomically precise, artificial oxide heterostructures allows for the possibility of producing exotic phases and enhanced susceptibilities not found in parent materials. Typical ferroelectric materials either exhibit large saturation polarization away from a phase boundary or large dielectric susceptibility near a phase boundary. Both large ferroelectric polarization and dielectric permittivity are attained wherein fully epitaxial (PbZr0.8Ti0.2O3)n/(PbZr0.4Ti0.6O3)2n (n = 2, 4, 6, 8, 16 unit cells) superlattices are produced such that the overall film chemistry is at the morphotropic phase boundary, but constitutive layers are not. Long‐ (n ≥ 6) and short‐period (n = 2) superlattices reveal large ferroelectric saturation polarization (Ps = 64 µC cm−2) and small dielectric permittivity (εr ≈ 400 at 10 kHz). Intermediate‐period (n = 4) superlattices, however, exhibit both large ferroelectric saturation polarization (Ps = 64 µC cm−2) and dielectric permittivity (εr = 776 at 10 kHz). First‐order reversal curve analysis reveals the presence of switching distributions for each parent layer and a third, interfacial layer wherein superlattice periodicity modulates the volume fraction of each switching distribution and thus the overall material response. This reveals that deterministic creation of artificial superlattices is an effective pathway for designing materials with enhanced responses to applied bias. 相似文献
56.
Julio Moreno Javier Gómez Manuel A. Serrano Eduardo B. Fernandez Eduardo Fernández-Medina 《Software》2020,50(8):1520-1538
Big Data environments are typically very complex ecosystems; this means that implementing them is complicated. One possible technique with which to address this complexity is the use of abstraction. Reference architecture (RA) can be useful for an improved understanding of the main components of Big Data. Herein, we propose a security RA that includes the management of security concerns and provides the main elements of a Big Data ecosystem. Application of this architecture to real-world scenarios facilitates its refinement and improves its usefulness. In this article, we present a case study of a real-world Big Data ecosystem implemented in a banking environment. This ecosystem was developed by everis, an NTT company with which we collaborated for this study. To conduct this validation case study, a map was established between the elements of the Big Data ecosystem implemented and our proposal. Consequently, a series of valuable lessons that can improve both our architecture and the security of the Big Data environment were obtained. These include recommendations for a set of best practices such as the use of security patterns. 相似文献
57.
Lopera J.M. Fernandez F.M. Diaz J. Prieto M.J. Pernia A.M. 《Industry Applications Magazine, IEEE》2002,8(2):34-38
Galvanic steel wire manufacturers have to fulfill several international regulations regarding zinc mass by surface unit. This article presents a new sensor to measure this parameter online. Details about the different parts of the system (sensor, circuitry, adjustment, and signal processing) are explained. Experimental results in a real manufacturing line are also presented. Online measurement conserves zinc mass and guarantees savings 相似文献
58.
59.
Marcelo Ozu Juan Jos Alvear-Arias Miguel Fernandez Agustín Caviglia Antonio Pea-Pichicoi Christian Carrillo Emerson Carmona Anselmo Otero-Gonzalez Jos Antonio Garate Gabriela Amodeo Carlos Gonzalez 《International journal of molecular sciences》2022,23(20)
Aquaporins (AQPs) are small transmembrane tetrameric proteins that facilitate water, solute and gas exchange. Their presence has been extensively reported in the biological membranes of almost all living organisms. Although their discovery is much more recent than ion transport systems, different biophysical approaches have contributed to confirm that permeation through each monomer is consistent with closed and open states, introducing the term gating mechanism into the field. The study of AQPs in their native membrane or overexpressed in heterologous systems have experimentally demonstrated that water membrane permeability can be reversibly modified in response to specific modulators. For some regulation mechanisms, such as pH changes, evidence for gating is also supported by high-resolution structures of the water channel in different configurations as well as molecular dynamics simulation. Both experimental and simulation approaches sustain that the rearrangement of conserved residues contributes to occlude the cavity of the channel restricting water permeation. Interestingly, specific charged and conserved residues are present in the environment of the pore and, thus, the tetrameric structure can be subjected to alter the positions of these charges to sustain gating. Thus, is it possible to explore whether the displacement of these charges (gating current) leads to conformational changes? To our knowledge, this question has not yet been addressed at all. In this review, we intend to analyze the suitability of this proposal for the first time. 相似文献
60.
Maria Luz Fernandez A. Karin Conde Laura R. Ruiz Carlos Montano John Ebner Donald J. McNamara 《Lipids》1995,30(7):619-626
To test the effects of exchanging dietary complex and simple carbohydrate for fat calories on lipoprotein metabolism, guinea
pigs were fed two different fat/carbohydrate ratios: 2.5∶58% (w/w) or 25∶29% (w/w) with either sucrose or starch as the carbohydrate
source. Animals fed high-fat had higher plasma low-density lipoprotein (LDL) and hepatic cholesterol concentrations than animals
fed low-fat diets (P<0.01). The cholesteryl ester content per particle was higher, and the number of triacylglycerol (TAG) molecules was lower
in very low density lipoprotein (VLDL) and LDL from animals fed high-fat diets. Intake of high-fat/sucrose resulted in higher
plasma LDL concentrations than intake of high-fat/starch, and animals fed low-fat/starch had the highest plasma TAG concentrations
associated with VLDL particles containing more TAG molecules, as well as a TAG-enriched LDL. The activity of plasma lecithin
cholesteryl:acyl transferase (LCAT) was highest in animals fed high-fat/sucrose, and heart lipoprotein lipase (LPL) activity
was higher in animals fed high-fat diets. Hepatic apoprotein B/E (apo B/E) receptor number (Bmax) was increased 21% with low-fat diets (P<0.01). These results suggest that the hypercholesterolemia induced by high-fat and by sucrose intake are associated with
a higher plasma LCAT activity which results in a cholesteryl ester-enriched VLDL which, by the action of LPL, might be more
readily converted to LDL through the delipidation cascade leading to downregulation of hepatic apo B/E receptors. The hypertriglyceridemia
associated with low-fat intake may result from increased production of VLDL TAG, which would explain the increased TAG content
and the higher TAG/CE ratio of VLDL from animals fed the low-fat/starch diet. 相似文献