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61.
P. Moçotéguy B. Ludwig D. Beretta T. Pedersen 《International Journal of Hydrogen Energy》2021,46(10):7475-7488
Reactants utilization is a key stake for a PEMFC system: a too low utilization leads to a waste of reactant but a too high utilization may result in a detrimental starvation. To study these impacts, two commercial stacks were characterised by impedance spectroscopy under different hydrogen and oxygen utilizations (from nominal conditions to quasi-starvation). One was fresh while the other was operated on-field during 10,000 h. This study shows that the two capacitive loops in the lowest frequency range (1 Hz and below) correspond respectively to oxygen and hydrogen mass transfer limitations: the limiting reactant can be clearly identified from the impacted frequencies. The size of these loops was increased by up to 30% when the cell operated at high reactant utilizations. These results could therefore pave the way to the development of algorithms able to estimate the degree of starvation of some cells. 相似文献
62.
Dr. techn. Dr. tekn. h.c. Prof. Emeritus J?rgen Bach Andersen M.Sc. Ph.D. Prof. Gert Fr?lund Pedersen 《e & i Elektrotechnik und Informationstechnik》2012,129(6):383-386
In future mobile communications many antennas will coexist on the device. This creates a number of problems related to bandwidth, efficiency, and correlation. Remedies are discussed in the paper, and attention is called to the promising area of adaptive matching, which also can solve problems related to the user. 相似文献
63.
Morten D. Pedersen 《风能》2019,22(1):124-139
The actuator disk is a well‐known and widely used theoretical tool in wind engineering. This work proposes a new theory based on an actuator surface, capable of treating time‐varying vectorial load distributions and yaw/pitch misalignment. A simplified representation of vortex motion is used to arrive at a tractable problem. The theory is not restricted to disks; arbitrary coplanar (optionally disjoint) actuator surfaces may be modeled. Some of the theoretical novelties used in the modeling includes use of the fractional Laplacian and extensive use of the Fourier transform on . Promising experimental validation based on pitch‐step experiments at the Tjæreborg turbine is furnished. Comparisons are also made to existing methodologies. Analysis and numerical work shows that the model encapsulates Coleman's vortex theory. 相似文献
64.
Dr. Mikayel Aznauryan Dr. Sofie Louise Noer Dr. Camilla W. Pedersen Dr. Jean-Louis Mergny Dr. Marie-Paule Teulade-Fichou Prof. Victoria Birkedal 《Chembiochem : a European journal of chemical biology》2021,22(10):1811-1817
Several small-molecule ligands specifically bind and stabilize G-quadruplex (G4) nucleic acid structures, which are considered to be promising therapeutic targets. G4s are polymorphic structures of varying stability, and their formation is dynamic. Here, we investigate the mechanisms of ligand binding to dynamically populated human telomere G4 DNA by using the bisquinolinium based ligand Phen-DC3 and a combination of single-molecule FRET microscopy, ensemble FRET and CD spectroscopies. Different cations are used to tune G4 polymorphism and folding dynamics. We find that ligand binding occurs to pre-folded G4 structures and that Phen-DC3 also induces G4 formation in unfolded single strands. Following ligand binding to dynamically populated G4s, the DNA undergoes pronounced conformational redistributions that do not involve direct ligand-induced G4 conformational interconversion. On the contrary, the redistribution is driven by ligand-induced G4 folding and trapping of dynamically populated short-lived conformation states. Thus, ligand-induced stabilization does not necessarily require the initial presence of stably folded G4s. 相似文献
65.
In this article, an extended version of the unified theory of acceptance and use of technology (UTAUT) is applied to explore the significance of various antecedents of acceptance of eight versions of a radio frequency identification (RFID) enabled service. The results show significant influences from performance expectancy, effort expectancy and technology anxiety on attitude to use RFID-enabled services, while facilitating conditions and attitude to use both have significant influences on intention to use the services. Looking into potential moderating influences, gender moderates most of the relationships in the model while age and experience with identification technology do not seem to be relevant moderators. Exploring the potential moderating influence of context experience, experience of the service context is found to moderate some of the relationships in the model. Managerial implications point to the importance of developing useful and user-friendly services and of communicating the user friendliness to potential customers to avoid the potential negative influence of technology anxiety. Service development and market communication should be sensitive to gender and context experience. 相似文献
66.
Heron Faggion Cristina Benincá Daniele Naviglio Fernando Augusto Pedersen Voll 《Chemical Engineering Communications》2017,204(3):406-413
A set of seven extraction experiments was performed to investigate the influence of pressure cycles on the kinetics of solute removal from leaves of mate dispersed in water. The mass ratio of liquid to dry solid (40), the temperature (32°C), and time of extraction (3600?s) were not varied. Five extraction runs were under cyclic pressurization (1?cycle?=?300?s at 91.4?kPa?+?300?s at 182.8?kPa) and stirring speeds (S) of 0, 150, 500, 1500, and 2000?rpm, while the two other ones were at constant pressure (182.8?kPa) and S close to 1500 and 2000?rpm. Based on seven pairs of parameters of a reliable second-order kinetic model (R2?≥?0.967), cyclic pressurization had no effect on equilibrium and kinetics of extraction (p?>?0.05) when the role of convection on solute transfer was negligible (S?≥?500?rpm). In the stirring speed range from 500 to 2000?rpm, the operation was controlled by diffusion (Bi >?1.7?×?103), so a transient two-dimensional diffusion model was able to describe correctly the changes of solute concentration with time. Below 500?rpm, solute transfer was governed by a combination of diffusion and convection with the external resistance to mass transfer as a function of S (16?≤?Bi?≤?28). 相似文献
67.
J.B. Clasen E. Norberg P. Madsen J. Pedersen M. Kargo 《Journal of dairy science》2017,100(8):6337-6342
Crossbreeding has been shown to improve the longevity of dairy cattle in countries across the world. The aim of this study was to estimate heterosis, breed effects, and genetic parameters for longevity in crossbred dairy cattle among Danish Holstein (DH), Danish Red (DR), and Danish Jersey (DJ) breeds. Data were provided from 119 Danish commercial herds that use systematic crossbreeding (i.e., rotational crossbreeding). Additional data from 11 mixed-breed herds with DH and DJ were included to estimate reliable breed effects for DJ. Survival information on 73,741 cows was analyzed with a linear animal model using the artificial insemination–REML algorithm in the DMU package. Five longevity (L) traits were defined: days from first calving until the end of first lactation or culling (L1), days from first calving until the end of second lactation or culling (L2), days from first calving until the end of third lactation or culling (L3), days from first calving until the end of fourth lactation or culling (L4), and days from first calving until the end of fifth lactation or culling (L5). Heritabilities ranged between 0.022 and 0.090. Additive breed effects in units of days were estimated relative to DH for DR as ?0.5 (L1), +10.5 (L2), +18.5 (L3), +11.9 (L4), and +28.6 (L5), and corresponding figures for DJ were +2.0, +0.5, +14.2, +27.7, and +44.0. Heterosis effects in L1 were low (1.2%) but favorable in crosses between DH and DR, whereas negative heterosis effects were estimated for crosses between DH and DJ (?2.5%) and DR and DJ (?1.2%). The largest heterosis effects for L2, L3, L4, and L5 were found in DH × DR and were favorable (+3.3, +5.7, +7.7, and +8.5%, respectively). Corresponding figures for heterosis effects in DH × DJ and DR × DJ were favorable as well: +2.3, +4.1, +5.6, and +6.2% in DH × DJ and +3.1, +7.3, +6.9, and +7.2% in DR × DJ. The favorable heterosis effects show that crossbreeding is an efficient tool for improving longevity in Danish dairy cattle. 相似文献
68.
Software failures in medical devices can lead to catastrophic situations. Therefore, it is crucial to handle software-related risks when developing medical devices, and there is a need for further analysis of how this type of risk management should be conducted. The objective of this paper is to collect and summarise experiences from conducting risk management with an organisation developing medical devices. Specific focus is put on the first steps of the risk management process, i.e. risk identification, risk analysis, and risk planning. The research is conducted as action research, with the aim of analysing and giving input to the organisation’s introduction of a software risk management process. First, the method was defined based on already available methods and then used. The defined method focuses on user risks, based on scenarios describing the expected use of the medical device in its target environment. During the use of the method, different stakeholders, including intended users, were involved. Results from the case study show that there are challenging problems in the risk management process with respect to definition of the system boundary and system context, the use of scenarios as input to the risk identification, estimation of detectability during risk analysis, and action proposals during risk planning. It can be concluded that the risk management method has potential to be used in the development organisation, although future research is needed with respect to, for example, context limitation and how to allow for flexible updates of the product. 相似文献
69.
Developing solar power generating resources on dormant, suspect property is an evolving activity, growing in popularity. 相似文献
70.
Importance of a Conserved Lys/Arg Residue for Ligand/PDZ Domain Interactions as Examined by Protein Semisynthesis
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Dr. Søren W. Pedersen Griffin E. Moran Vita Sereikaitė Dr. Linda M. Haugaard‐Kedström Prof. Dr. Kristian Strømgaard 《Chembiochem : a European journal of chemical biology》2016,17(20):1936-1944
PDZ domains are ubiquitous small protein domains that are mediators of numerous protein–protein interactions, and play a pivotal role in protein trafficking, synaptic transmission, and the assembly of signaling‐transduction complexes. In recent years, PDZ domains have emerged as novel and exciting drug targets for diseases (in the brain in particular), so understanding the molecular details of PDZ domain interactions is of fundamental importance. PDZ domains bind to a protein partner at either a C‐terminal peptide or internal peptide motifs. Here, we examined the importance of a conserved Lys/Arg residue in the ligand‐binding site of the second PDZ domain of PSD‐95, by employing a semisynthetic approach. We generated six semisynthetic PDZ domains comprising different proteogenic and nonproteogenic amino acids representing subtle changes of the conserved Lys/Arg residue. These were tested with four peptide interaction partners, representing the two different binding modes. The results highlight the role of a positively charged amino acid in the β1–β2 loop of PDZ domains, and show subtle differences for canonical and noncanonical interaction partners, thus providing additional insight into the mechanism of PDZ/ligand interaction. 相似文献