Investigation of the Gas‐Liquid Flow in a Stopper‐Rod Controlled SEN

The behaviour of two‐phase gas‐liquid flows in a stopper‐rod controlled submerged entry nozzle (SEN) is investigated in water model experiments. The observed two‐phase flow patterns can be classified into either bubble coring or bubbly slug. The scaling of the two‐phase flows by means of similarity parameters is discussed. In the experiments, it is found that the liquid flow rates depend strongly on the two‐phase flow patterns. Additionally, the influence of swirl on the flow patterns is investigated in detail. It is shown that swirl has a marked impact on the transition from bubble coring to bubbly slug. Finally, an estimation of the two‐phase argon‐steel flow patterns in industrialscale SEN flows is given.     

Evaluating the effectiveness factor from a 1D approximation fitted at high Thiele modulus: Spanning commercial pellet shapes with linear kinetics

In the present contribution a one-dimensional model (1D model) is employed to account for the effect of pellet shape in the solution of diffusion-catalytic reaction problems. The 1D model free parameter (σ) is fitted so as to match the second term of the series for the effective reaction rate at fast kinetic conditions. The performance of the model was evaluated for a large collection of pellets available commercially, covering almost all the main shapes offered by catalyst manufacturers. For isothermal linear kinetics the effectiveness factors calculated by the 1D model differ at most by 3% from those of the actual 3D pellets.     

MR high-resolution imaging of arterial intimal hyperplasia at 1.5 T

Intimal hyperplasia is the earliest microscopic change detectable in the arterial wall in the development of arteriosclerosis and atherosclerosis. To enable high-resolution imaging of this, a standard 1.5-T machine was modified using inserted gradient coils of 30 cm internal diameter and surface radio-frequency coils. Six rabbit aorta specimens with injury-induced intimal hyperplasia and two normal rabbit aorta specimens were imaged using a spin-echo sequence of TE 40. Pixel sizes as small as 20 µ×20 µ could be obtained and the area of intimal hyperplasia could be measured at 80 µ×40 µ. The results were compared with those made by a computer-linked microscope. In the injured aortas, there was a low-signal region on magnetic resonance imaging which corresponded to the media of the vessel wall when compared with the histology.T ₁ andT ₂ constants for the media were determined and compared with those of a normal specimen. TheT ₁ constant was shorter for the media of the injured aorta than in the normal. This could be related to the disruption of the internal elastic lamina and migration of muscle cells to the intima which occurs following injury. The popliteal artery of four normal volunteers was imaged and the area of the wall could be measured at a pixel size of 312 µ×160 µ. This suggests thatin vivo studies of intimal hyperplasia arteriosclerosis and atherosclerosis could be made.     

The HIV-inactivating protein, cyanovirin-N, does not block gp120-mediated virus-to-cell binding

Concentrations of the potent, HIV(human immunodeficiency virus) inactivating protein, cyanovirin-N (CV-N), which completely inhibit HIV-1 infectivity, do not block the binding of soluble CD4-receptor (sCD4) to HIV-1 lysates nor the attachment of intact HIV-1 virions to several target T-cell lines. Furthermore, in contrast to the known disassociative effects of sCD4 on viral envelope glycoproteins, treatment of HIVRF with high concentrations of CV-N results in complete viral inactivation but without apparent shedding of gp120 or other ultrastructural changes. These results are consistent with the view that the virucidal effects of CV-N result from interference with step(s) in the fusion process subsequent to the initial binding of the virus to target cells.     

Impact on human health of Salmonella spp. on pork in The Netherlands and the anticipated effects of some currently proposed control strategies

Cell-cell and cell-extracellular matrix interactions are fundamental processes involved in cell migration and tissue remodeling. Both the cyclic regeneration of the human endometrium during the menstrual cycle as well as the process of embryo implantation involve such dynamic interactions. It has become quite clear that integrin adhesion molecules expressed on the surface of cells play critical roles in the transmission of signals from the extracellular milieu to the cells. It is these signals that presumably regulate the behavior of these cells during major morphogenetic processes. In recent years, work in human endometrium and trophoblasts has uncovered both the regulated and constitutive expression of integrin subunits and their extracellular matrix ligands in these tissues. In addition, attempts have been made to correlate pathological states related to either infertility or abnormal pregnancy to the aberrant expression of several of these integrins. The purpose of the present review is to describe briefly our present state of knowledge of the expression of integrins in human endometrium and trophoblasts and provide the reader with the necessary background needed to understand, at the cellular and molecular levels, processes in reproduction such as embryo implantation.     

Nasal Epithelial Cell-Based Models for Individualized Study in Cystic Fibrosis

The emergence of highly effective CFTR modulator therapy has led to significant improvements in health care for most patients with cystic fibrosis (CF). For some, however, these therapies remain inaccessible due to the rarity of their individual CFTR variants, or due to a lack of biologic activity of the available therapies for certain variants. One proposed method of addressing this gap is the use of primary human cell-based models, which allow preclinical therapeutic testing and physiologic assessment of relevant tissue at the individual level. Nasal cells represent one such tissue source and have emerged as a powerful model for individual disease study. The ex vivo culture of nasal cells has evolved over time, and modern nasal cell models are beginning to be utilized to predict patient outcomes. This review will discuss both historical and current state-of-the art use of nasal cells for study in CF, with a particular focus on the use of such models to inform personalized patient care.