Endometrial Epithelial ARID1A Is Required for Uterine Immune Homeostasis during Early Pregnancy

A growing body of work suggests epigenetic dysregulation contributes to endometriosis pathophysiology and female infertility. The chromatin remodeling complex subunit AT-rich interaction domain 1A (ARID1A) must be properly expressed to maintain normal uterine function. Endometrial epithelial ARID1A is indispensable for pregnancy establishment in mice through regulation of endometrial gland function; however, ARID1A expression is decreased in infertile women with endometriosis. We hypothesized that ARID1A performs critical operations in the endometrial epithelium necessary for fertility besides maintaining gland function. To identify alterations in uterine gene expression resulting from loss of epithelial ARID1A, we performed RNA-sequencing analysis on pre-implantation uteri from Ltf^iCre/+Arid1a^f/f and control mice. Differential expression analysis identified 4181 differentially expressed genes enriched for immune-related ingenuity canonical pathways including agranulocyte adhesion and diapedesis and natural killer cell signaling. RT-qPCR confirmed an increase in pro-inflammatory cytokine and macrophage-related gene expression but a decrease in natural killer cell signaling. Immunostaining confirmed a uterus-specific increase in macrophage infiltration. Flow cytometry delineated an increase in inflammatory macrophages and a decrease in uterine dendritic cells in Ltf^iCre/+Arid1a^f/f uteri. These findings demonstrate a role for endometrial epithelial ARID1A in suppressing inflammation and maintaining uterine immune homeostasis, which are required for successful pregnancy and gynecological health.     

Characterization of the Secretome of a Specific Cell Expressing Mutant Methionyl-tRNA Synthetase in Co-Culture Using Click Chemistry

Co-culture system, in which two or more distinct cell types are cultured together, is advantageous in that it can mimic the environment of the in vivo niche of the cells. In this study, we presented a strategy to analyze the secretome of a specific cell type under the co-culture condition in serum-supplemented media. For the cell-specific secretome analysis, we expressed the mouse mutant methionyl-tRNA synthetase for the incorporation of the non-canonical amino acid, azidonorleucine into the newly synthesized proteins in cells of which the secretome is targeted. The azidonorleucine-tagged secretome could be enriched, based on click chemistry, and distinguished from any other contaminating proteins, either from the cell culture media or the other cells co-cultured with the cells of interest. In order to have more reliable true-positive identifications of cell-specific secretory bodies, we established criteria to exclude any identified human peptide matched to bovine proteins. As a result, we identified a maximum of 719 secreted proteins in the secretome analysis under this co-culture condition. Last, we applied this platform to profile the secretome of mesenchymal stem cells and predicted its therapeutic potential on osteoarthritis based on secretome analysis.     

Molecular dynamics study on nanotube-resonators with mass migration applicable to both frequency-tuner and data-storage-media

We investigated the cantilevered carbon-nanotube-resonator including electromigratively movable nanoparticle via classical molecular dynamics simulations and continuum model. The change of the effective mass value, which was closely correlated with the position change of the encapsulated nanoparticle, could be regressed by a power function, the resonance frequency of the carbon-nanotube-resonator could be tuned by controlling the nanoparticle’s position, and the possible frequency-shift-ranges then reached 18–85%. The suggested device could be served as a data-storage-media for electromechanical nonvolatile-memory as well as a frequency-tuner.     

A risk management approach to safety assessment of trenchless technologies for culvert rehabilitation

Proper functioning of culverts is not only critical to protecting the transportation system from flooding but also critical to maintaining the expected service life of the overall transportation system. During the service life of culverts, they deteriorate due to many reasons such as corrosion caused by road salt, blockage by debris and sediment, poor construction techniques, etc. Trenchless technologies have been used by several State Departments of Transportation (DOTs) to rehabilitate deteriorated drainage structures without disrupting the traffic and within a relatively short span of time. These technologies provide many benefits in terms of cost, time, quality, and expected service life. However, the safety aspects of these techniques have not been discussed and documented in detail probably due to the complexity and the lack of safety standards and specifications directly associated with the techniques. This paper discusses the application of a risk assessment framework to assess potential safety issues of currently available trenchless technologies for culvert rehabilitation. The findings of the study will provide additional information to improve the decision making process in selecting and planning culvert rehabilitation projects.     

Stretchable,Transparent Zinc Oxide Thin Film Transistors

Stretchable and transparent thin film transistors (TFTs) with intrisically brittle oxide semiconductors are built using a wavy structural configuration that can provide high flexibility and stretchability. After device fabrication procedures including high temperature annealing, the oxide semiconductor‐based TFT arrays can be transferred directly to plastic or rubber substrates, without an additional device process, using transfer printing methods. This procedure can avoid some of the thermal degradation problems associated with plastic or rubber substrates by separating them from the annealing procedure needed to improve the device performance. These design and fabrication methods offer the possibility of developing a new format of stretchable electronics.     

Fast Super‐Resolution Algorithm Based on Dictionary Size Reduction Using k‐Means Clustering

This paper proposes a computationally efficient learning‐based super‐resolution algorithm using k‐means clustering. Conventional learning‐based super‐resolution requires a huge dictionary for reliable performance, which brings about a tremendous memory cost as well as a burdensome matching computation. In order to overcome this problem, the proposed algorithm significantly reduces the size of the trained dictionary by properly clustering similar patches at the learning phase. Experimental results show that the proposed algorithm provides superior visual quality to the conventional algorithms, while needing much less computational complexity.     

Does Multitasking Increase or Decrease Persuasion? Effects of Multitasking on Comprehension and Counterarguing

This study examined the effects of multitasking on persuasion, including comprehension and counterarguing of persuasive messages, which were presented in three different contexts: (a) nonmultitasking with full attention paid to the message, (b) multitasking with primary attention paid to the message, and (c) multitasking with secondary attention paid to the message. Consistent with predictions, the results suggested that multitasking reduced the actual and perceived levels of comprehension and also reduced counterarguing. The implications for research on persuasion are further discussed.     

Streaming Media and Multimedia Conferencing Traffic Analysis Using Payload Examination

This paper presents a method and architecture to analyze streaming media and multimedia conferencing traffic. Our method is based on detecting the transport protocol and port numbers that are dynamically assigned during the setup between communicating parties. We then apply such information to analyze traffic generated by the most popular streaming media and multimedia conferencing applications, namely, Windows Media, Real Networks, QuickTime, SIP and H.323. We also describe a prototype implementation of a traffic monitoring and analysis system that uses our method and architecture.     

Holography and plasma oxidation for uniform nanoscale two dimensional channel formation of vertical organic field-effect transistors with suppressed gate leakage current

Vertical organic field-effect transistors (VOFETs) with nanoscale channel openings have been fabricated using pentacene as an active layer material. To achieve uniform nanoscale two-dimensional channel openings, a laser holography lithography has been introduced. Uniformly distributed and well-aligned holes with 250 nm diameter were successfully obtained with the laser holography lithography. VOFET devices with these channel openings have shown high on/off ratio of about 10³ without any further treatment. Gate leakage current was also decreased with an additional insulating layer generated on the gate electrode sidewall via plasma oxidation.     

Stress Mechanism about Field Lightning Surge of High Voltage BJT Based Line Driver for ADSL System

In this paper, we have verified stress root caused by lightning surge in High voltage BJT based line driver of ADSL telecommunication and created failure mechanism. To reproduce damages in Line driver, we have applied STD surge waveform in operating condition, which is specified in IEC-6000-4-5, to component and board level. Visual isolation for Damage root was conducted with Real-time Electrical Stress Analysis (RTESA) utilizing Photon Emission Microscopy (PEM). The surge made with input of Tx output created junction breakdown of amplifier, which is made of HVBJT cells, and also caused current crowding from Vcc (supply voltage). In case of Positive pulse, current crowding was observed from between Collector and Vcc (+)12V. For negative pulse, at between Emitter and Vee (−)12V, current crowding was found. For both cases, device damage level was the same. All of these could be considered as transient latchup phenomenon created in BJT cell. Applying temporary clamping diode to line driver Tx, we have conducted Board level surge injection test. As a result, with the correlation between surge level from component level reproduction test and the one from system level, we have decided field lightning surge damage level and set up line driver protection margin.