Use of a sex attractant and an inhibitor for monitoring winter moth and bruce spanworm populations

A single sex pherormone component was isolated from abdominal tip extracts of female Bruce spanworm.Operophtera bruceata (Hulst). This was identified as (Z,Z,Z)-1,3,6,9-nonadecatetraene by capillary gas chromatography (GC), electroantennography, and mass spectrometry. In addition, hexane extracts of female abdominal tips from Bruce spanworm and the winter moth.O. brumata L., were analyzed by GC coupled to an electroantennographic detector (GC-EAD). The extracts ofO. bruceata andO. brumata females elicited only a single response, at the same retention time, from antennae of their conspecific and reciprocal males. In field tests conducted in Saskatchewan, traps baited with the synthetic tetraene captured Bruce spanworm males. In tests carried out on Vancouver Island, British Columbia, where the two species coexist, both Bruce spanworm and winter moth males were captured. The attractancy of lures containing the synthetic pheromone alone and in combination with several structurally related analogs was field tested at both locations. One of these, an isomer of the natural pheromone, (E,Z,Z)-1,3,6,9-nonadecatetraene, inhibited the capture of Bruce spanworm males but had no effect upon the number of winter moth males which were taken. Thus, populations of these two very similar species can be distinguished by employing traps baited with pheromone ± the inhibitor. Coneorifice Hara traps were found useful for field trapping males of both species.Issued as NRCC No. 26107     

In-focus electron microscopy of frozen-hydrated biological samples with a Boersch phase plate

We report the implementation of an electrostatic Einzel lens (Boersch) phase plate in a prototype transmission electron microscope dedicated to aberration-corrected cryo-EM. The combination of phase plate, C_s corrector and Diffraction Magnification Unit (DMU) as a new electron-optical element ensures minimal information loss due to obstruction by the phase plate and enables in-focus phase contrast imaging of large macromolecular assemblies. As no defocussing is necessary and the spherical aberration is corrected, maximal, non-oscillating phase contrast transfer can be achieved up to the information limit of the instrument. A microchip produced by a scalable micro-fabrication process has 10 phase plates, which are positioned in a conjugate, magnified diffraction plane generated by the DMU. Phase plates remained fully functional for weeks or months. The large distance between phase plate and the cryo sample permits the use of an effective anti-contaminator, resulting in ice contamination rates of <0.6 nm/h at the specimen. Maximal in-focus phase contrast was obtained by applying voltages between 80 and 700 mV to the phase plate electrode. The phase plate allows for in-focus imaging of biological objects with a signal-to-noise of 5-10 at a resolution of 2-3 nm, as demonstrated for frozen-hydrated virus particles and purple membrane at liquid-nitrogen temperature.     

Equaliser Performance for HIPERLAN in indoor channels

In order to accomplish practical deployment modelling for system performance evaluation and comparison for possible modulation and equalisation schemes to be used in HIPERLAN, a wide band tapped delay line (WTDL) channel model has been adopted by ETSI to characterise the multipath fading in the indoor radio environment. Based on this statistical channel model, and using Monte Carlo method, this paper evaluates the average probability of error for linear and decision feedback equaliser as a function of signal-to-noise ratio. It also evaluates the matched filter bound for this channel model. The results show the optimum performance levels achievable via the use of any equaliser.The work described in this paper was supported by the UK DTI/EPSRC LINK project: PC2011 High Throughput Radio Modem under EPSRC grant reference GR/K00318 in collaboration with Symbionics Networks limited.     

Electromagnetic-field calculations for irregularly shaped, axisymmetric layered particles with focused illumination

Theoretical procedures are presented for the determination of the internal and the near-surface electromagnetic fields for an arbitrary monochromatic field (e.g., a focused laser beam) incident upon an irregulary shaped, axisymmetric layered particle. The layered spherical particle solution is also given as a special case of the general solution. Systematic calculations are presented that demonstrate the effects of particle shape and incident focused-beam orientation on the electromagnetic-field distributions.     

Paging tradeoffs in distributed-shared-memory multiprocessors

Massively parallel processors have begun using commodity operating systems that support demand-paged virtual memory. To evaluate the utility of virtual memory, we measured the behavior of seven shared-memory parallel application programs on a simulated distributed-shared-memory machine. Our results (1) confirm the importance of gang CPU scheduling, (2) show that a page-faulting processor should spin rather than invoke a parallel context switch, (3) show that our parallel programs frequently touch most of their data, and (4) indicate that memory, not just CPUs, must be gang scheduled. Overall, our experiments demonstrate that demand paging has limited value on current parallel machines because of the applications' synchronization and memory reference patterns and the machines' high page-fault and parallel context-switch overheads.An earlier version of this paper was presented at Supercomputing '94.This work is supported in part by NSF Presidential Young Investigator Award CCR-9157366; NSF Grants MIP-9225097, CCR-9100968, and CDA-9024618; Office of Naval Research Grant N00014-89-J-1222; Department of Energy Grant DE-FG02-93ER25176; and donations from Thinking Machines Corporation, Xerox Corporation, and Digital Equipment Corporation.     

A rapid computational filter for predicting the rate of human renal clearance

In silico models that predict the rate of human renal clearance for a diverse set of drugs, that exhibit both active secretion and net re-absorption, have been produced using three statistical approaches. Partial Least Squares (PLS) and Random Forests (RF) have been used to produce continuous models whereas Classification And Regression Trees (CART) has only been used for a classification model. The best models generated from either PLS or RF produce significant models that can predict acids/zwitterions, bases and neutrals with approximate average fold errors of 3, 3 and 4, respectively, for an independent test set that covers oral drug-like property space. These models contain additional information on top of any influence arising from plasma protein binding on the rate of renal clearance. Classification And Regression Trees (CART) has been used to generate a classification tree leading to a simple set of Renal Clearance Rules (RCR) that can be applied to man. The rules are influenced by lipophilicity and ion class and can correctly predict 60% of an independent test set. These percentages increase to 71% and 79% for drugs with renal clearances of < 0.1 ml/min/kg and > 1 ml/min/kg, respectively. As far as the authors are aware these are the first set of models to appear in the literature that predict the rate of human renal clearance and can be used to manipulate molecular properties leading to new drugs that are less likely to fail due to renal clearance.     

Walk-up VR: virtual reality beyond projection screens

Today, VR research and development efforts often focus on the continual innovation of interaction styles and metaphors for virtual environments (VEs). New tools and interaction devices aim to increase the immersive experience rather than support seamless integration with real work scenarios. Even though users may soon perceive odours within VEs, real, task-oriented interaction within these environments will continue to lag behind. Combining these efforts can result in new user interfaces that reduce the cumbersome barriers prevalent in VEs today, finally unleashing the latent impact of this technology in everyday life. Implementing this vision requires an interdisciplinary and applied approach to integrate VR into the workplace. Mixed-reality display capabilities, useful multimodal interaction and perceptual, intuitive interfaces are major components of such an application-oriented and human-centered approach, for which we coined the term walk-up VR. The paper discusses augmented and virtual reality as contributing technologies     

Radiopharmacology of inhaled 133Xe in skeletal sites containing deposits of Gaucher cells

Gaucher's disease is a lysosomal storage disease in which cells of the reticuloendothelial system accumulate the lipid glucocerebroside. It is characterized by slowly progressive visceral and osseous involvement. One of the latter manifestations includes lipid infiltration of bone marrow. We monitored the rate of inhaled 133Xe uptake and wash-out over diseased and normal metaphyseal and epiphyseal areas of the knee. Twenty-two patients (15 adults, 7 children) with various degrees of previously diagnosed Gaucher's disease were positioned supine under a gamma-camera interfaced to a computer system. All patients rebreathed 133Xe gas from a closed system for 10 min followed by 14 min of wash-out. Digitized images of the lung, liver, spleen, bony sites and soft tissue were obtained at 1 min intervals during the wash-in and wash-out phases. Counts for each ROI were normalized per 100 pixels and plotted as a function (time). Maximum uptake was also calculated by relating the counts/ROI/100 pixels to the 10 min integrated lung count during equilibrium (the administered "dose"). There was essentially no 133Xe uptake in liver and spleen involved with Gaucher's disease. Monophasic uptake and biphasic wash-out curves were observed in the limited investigative population. Skeletal Gaucher deposits released the 133Xe at a greater rate relative to soft tissue.     

A study of the polymerization behaviour of N-(4-phenoxy)-phenylmaleimide using DSC analysis

Summary The thermal cure of a monofunctional maleimide N-(4-phenoxy)-phenylmaleimid (1) was examined using differential scanning calorimetry (DSC). The analogous bis-maleimide bis-(4-maleimidophenyl)ether (2) was also analyzed in the same manner for comparison. Polymerization kinetics derived from scanning DSC measurements showed an order of reaction varying between n=1 and 2 for (1), compared to an order of reaction between n=2 and 3 (by multiple linear regression analysis) for (2). The overall enthalpy of polymerization (H_o) for N-(4-phenoxy)-phenylmaleimide (94kJ/mole) agrees well with that obtained for maleimide itself (89kJ/mole) using conventional calorimetric techniques indicating that polymerization appears complete within the timescale of the experiment. In contrast the analogous BMI displays a markedly lower overall polymerization enthalpy (ca. 66kJ/mole) indicating an apparent conversion of only 37%.     

University/corporate research agreements

The author examines the evolving university-industry relationships in the biotechnology area, addressing the conceptual basis for such arrangements and the resultant contract issues. He examines the need for development of policies in academia that will accommodate the needs of both government and industry without harming the integrity or institutional objectives of the university. The author then recommends a number of directives for written rules to help achieve these objectives, especially ones relating to patenting and licensing of inventions developed under industrial support.