全文获取类型
收费全文 | 300篇 |
免费 | 16篇 |
专业分类
电工技术 | 2篇 |
化学工业 | 77篇 |
机械仪表 | 1篇 |
建筑科学 | 18篇 |
能源动力 | 11篇 |
轻工业 | 50篇 |
水利工程 | 3篇 |
无线电 | 18篇 |
一般工业技术 | 47篇 |
冶金工业 | 38篇 |
自动化技术 | 51篇 |
出版年
2024年 | 1篇 |
2023年 | 3篇 |
2022年 | 6篇 |
2021年 | 13篇 |
2020年 | 4篇 |
2019年 | 13篇 |
2018年 | 15篇 |
2017年 | 6篇 |
2016年 | 12篇 |
2015年 | 11篇 |
2014年 | 11篇 |
2013年 | 17篇 |
2012年 | 28篇 |
2011年 | 21篇 |
2010年 | 16篇 |
2009年 | 10篇 |
2008年 | 17篇 |
2007年 | 13篇 |
2006年 | 13篇 |
2005年 | 10篇 |
2004年 | 3篇 |
2003年 | 5篇 |
2002年 | 9篇 |
2001年 | 2篇 |
2000年 | 4篇 |
1999年 | 5篇 |
1998年 | 13篇 |
1997年 | 10篇 |
1996年 | 5篇 |
1995年 | 2篇 |
1994年 | 2篇 |
1993年 | 1篇 |
1992年 | 3篇 |
1991年 | 1篇 |
1989年 | 1篇 |
1986年 | 1篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1981年 | 1篇 |
1978年 | 1篇 |
1976年 | 1篇 |
1975年 | 1篇 |
1972年 | 2篇 |
排序方式: 共有316条查询结果,搜索用时 15 毫秒
31.
Simultaneous quantitative analysis of metabolites using ion-pair liquid chromatography-electrospray ionization mass spectrometry 总被引:1,自引:0,他引:1
Coulier L Bas R Jespersen S Verheij E van der Werf MJ Hankemeier T 《Analytical chemistry》2006,78(18):6573-6582
We have developed an analytical method, consisting of ion-pair liquid chromatography coupled to electrospray ionization mass spectrometry (IP-LC-ESI-MS), for the simultaneous quantitative analysis of several key classes of polar metabolites, like nucleotides, coenzyme A esters, sugar nucleotides, and sugar bisphosphates. The use of the ion-pair agent hexylamine and optimization of the pH of the mobile phases were critical parameters in obtaining good retention and peak shapes of many of the above-mentioned polar and acidic metabolites that are impossible to analyze using standard reversed-phase LC/MS. Optimum conditions were found when using a gradient from 5 mM hexylamine in water (pH 6.3) to 90% methanol/10% 10 mM ammonium acetate (pH 8.5). The IP-LC-ESI-MS method was extensively validated by determining the linearity (R2 > 0.995), sensitivity (limit of detection 0.1-1 ng), repeatability, and reproducibility (relative standard deviation <10%). The IP-LC-ESI-MS method was shown to be a useful tool for microbial metabolomics, i.e., the comprehensive quantitative analysis of metabolites in extracts of microorganisms, and for the determination of the energy charge, i.e., the cellular energy status, as an overall quality measure for the sample workup and analytical protocols. 相似文献
32.
33.
Rokers Bas; Mercado Eduardo III; Allen M. Todd; Myers Catherine E.; Gluck Mark A. 《Canadian Metallurgical Quarterly》2002,116(1):48
Septohippocampal interactions determine how stimuli are encoded during conditioning. This study extends a previous neurocomputational model of corticohippocampal processing to incorporate hippocamposeptal feedback and examines how the presence or absence of such feedback affects learning in the model. The effects of septal modulation in conditioning were simulated by dynamically adjusting the hippocampal learning rate on the basis of how well the hippocampal system encoded stimuli. The model successfully accounts for changes in behavior and septohippocampal activity observed in studies of the acquisition, retention, and generalization of conditioned responses and accounts for the effects of septal disruption on conditioning. The model provides a computational, neurally based synthesis of prior learning theories that predicts changes in medial septal activity based on the novelty of stimulus events. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
34.
Stumpo Vittorio Sebök Martina van Niftrik Christiaan Hendrik Bas Seystahl Katharina Hainc Nicolin Kulcsar Zsolt Weller Michael Regli Luca Fierstra Jorn 《Magma (New York, N.Y.)》2022,35(1):29-44
Magnetic Resonance Materials in Physics, Biology and Medicine - Innovative physiologic MRI development focuses on depiction of heterogenous vascular and metabolic features in glioblastoma. For this... 相似文献
35.
36.
Jinmi Zou Frauke Swieringa Bas de Laat Philip G. de Groot Mark Roest Johan W. M. Heemskerk 《International journal of molecular sciences》2022,23(20)
Integrin αIIbβ3 activation is essential for platelet aggregation and, accordingly, for hemostasis and arterial thrombosis. The αIIbβ3 integrin is highly expressed on platelets and requires an activation step for binding to fibrinogen, fibrin or von Willebrand factor (VWF). A current model assumes that the process of integrin activation relies on actomyosin force-dependent molecular changes from a bent-closed and extended-closed to an extended-open conformation. In this paper we review the pathways that point to a functional reversibility of platelet αIIbβ3 activation and transient aggregation. Furthermore, we refer to mouse models indicating that genetic defects that lead to reversible platelet aggregation can also cause instable thrombus formation. We discuss the platelet agonists and signaling pathways that lead to a transient binding of ligands to integrin αIIbβ3. Our analysis points to the (autocrine) ADP P2Y1 and P2Y12 receptor signaling via phosphoinositide 3-kinases and Akt as principal pathways linked to reversible integrin activation. Downstream signaling events by protein kinase C, CalDAG-GEFI and Rap1b have not been linked to transient integrin activation. Insight into the functional reversibility of integrin activation pathways will help to better understand the effects of antiplatelet agents. 相似文献
37.
Mangrove forests as a nature-based solution for coastal flood protection: Biophysical and ecological considerations 下载免费PDF全文
Rosanna van Hespen Zhan Hu Bas Borsje Michela De Dominicis Daniel A. Friess Svetlana Jevrejeva Maarten G. Kleinhans Maria Maza Celine E. J. van Bijsterveldt Tom Van der Stocken Bregje van Wesenbeeck Danghan Xie Tjeerd J. Bouma 《水科学与水工程》2023,16(1):1-13
Nature-based coastal protection is increasingly recognised as a potentially sustainable and cost-effective solution to reduce coastal flood risk. It uses coastal ecosystems such as mangrove forests to create resilient designs for coastal flood protection. However, to use mangroves effectively as a nature-based measure for flood risk reduction, we must understand the biophysical processes that govern risk reduction capacity through mangrove ecosystem size and structure. In this perspective, we evaluate the current state of knowledge on local physical drivers and ecological processes that determine mangrove functioning as part of a nature-based flood defence. We show that the forest properties that comprise coastal flood protection are well-known, but models cannot yet pinpoint how spatial heterogeneity of the forest structure affects the capacity for wave or surge attenuation. Overall, there is relatively good understanding of the ecological processes that drive forest structure and size, but there is a lack of knowledge on how daily bed-level dynamics link to long-term biogeomorphic forest dynamics, and on the role of combined stressors influencing forest retreat. Integrating simulation models of forest structure under changing physical (e.g. due to sea-level change) and ecological drivers with hydrodynamic attenuation models will allow for better projections of long-term natural coastal protection. 相似文献
38.
Yanna Vogiazou Bas Raijmakers Erik Geelhoed Josephine Reid Marc Eisenstadt 《Personal and Ubiquitous Computing》2007,11(1):45-58
In this paper we present our work in the design of ubiquitous social experiences, aiming to foster group participation and spontaneous playful behaviours in a city environment. We outline our approach of design for emergence: to provide just enough of a game context and challenge for people to be creative, to extend and enrich the experience of play through their interaction in the real world. CitiTag is our mixed reality testbed, a wireless location-based multiplayer game based on the concept of playground ‘tag’. We describe the design and implementation of CitiTag and discuss results from two user studies. 相似文献
39.
The paper is focused on the evolution of the watermarking schemes from pixel-based approaches to content based methods. Firstly, the field of watermarking is presented, its implication in the real word is outlined. The second section gives a brief overview of different approaches in the spatial and the frequential domain. We then present different content-based approaches used to improve the invisibility or the synchronisation of the mark. The last section describes our content-based scheme; the synchronisation of the mark after image processing lies in the use of feature point detectors. The image is decomposed into a set of triangles and each triangle is individually marked. 相似文献
40.