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排序方式: 共有622条查询结果,搜索用时 15 毫秒
161.
162.
Pelagie M. Favi Roberto S. Benson Nancy R. Neilsen Ryan L. Hammonds Cassandra C. Bates Christopher P. Stephens Madhu S. Dhar 《Materials science & engineering. C, Materials for biological applications》2013,33(4):1935-1944
The culture of multipotent mesenchymal stem cells on natural biopolymers holds great promise for treatments of connective tissue disorders such as osteoarthritis. The safety and performance of such therapies relies on the systematic in vitro evaluation of the developed stem cell-biomaterial constructs prior to in vivo implantation. This study evaluates bacterial cellulose (BC), a biocompatible natural polymer, as a scaffold for equine-derived bone marrow mesenchymal stem cells (EqMSCs) for application in bone and cartilage tissue engineering. An equine model was chosen due to similarities in size, load and types of joint injuries suffered by horses and humans. Lyophilized and critical point dried BC hydrogel scaffolds were characterized using scanning electron microscopy (SEM) to confirm nanostructure morphology which demonstrated that critical point drying induces fibre bundling unlike lyophilisation. EqMSCs positively expressed the undifferentiated pluripotent mesenchymal stem cell surface markers CD44 and CD90. The BC scaffolds were shown to be cytocompatible, supporting cellular adhesion and proliferation, and allowed for osteogenic and chondrogenic differentiation of EqMSCs. The cells seeded on the BC hydrogel were shown to be viable and metabolically active. These findings demonstrate that the combination of a BC hydrogel and EqMSCs are promising constructs for musculoskeletal tissue engineering applications. 相似文献
163.
Jia Liu Hung-Jue Sue Zachary J. Thompson Frank S. Bates Marv Dettloff George Jacob Nikhil Verghese Ha Pham 《Polymer》2009,50(19):1191-565
Model diglycidyl ether of bisphenol-A based epoxy resins containing well-dispersed 15 nm block copolymer (BCP) nanoparticles were prepared to study the effect of matrix crosslink density on their fracture behavior. The crosslink density of the model epoxies was varied via the controlled epoxy thermoset technology and estimated experimentally. As expected, it was found that the fracture toughness of the BCP-toughened epoxy is strongly influenced by the crosslink density of the epoxy matrix, with higher toughenability for lower crosslink density epoxies. Key operative toughening mechanisms of the above model BCP-toughened epoxies were found to be nanoparticle cavitation-induced matrix shear banding for the low crosslink density epoxies. The toughening effect from BCP nanoparticles was also compared with core-shell rubber-toughened epoxies having different levels of crosslink density. The usefulness of the present findings for designing toughened thermosetting materials with desirable properties is discussed. 相似文献
164.
Charles Hoppin Robert B. Bates Cristina G. Contreras Arpad Somogyi Matthew J. Streeter H. K. Hall Jr. 《Polymer Bulletin》2009,63(4):509-515
Poly(dibenzodiazocine)s, members of a new class of polyimines with high thermal stability and solubility in organic solvents, were synthesized from 2-amino-4′-fluorobenzophenone and three bisphenols. The high molecular weight polymers were shown by MALDI-TOF mass spectra to be accompanied by low molecular weight linear oligomers with the expected end group combinations and low molecular weight cyclic oligomers. The yields of the smaller cyclics were raised using a pseudo-dilution polymerization method. 相似文献
165.
“如果这些投资项目不能获得资金,这些边际业务案例中的一些不能进行下去,我认为这将是件好事。我大体上是一个乐观主义者,我认为信贷市场将重归正常并完全起作用。” 相似文献
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Discrete nanopores develop in the interfacial regions of polyolefin blends and layered films under tension. They originate from crazelike structures in the early development stage, which later undergo disruption processes with an increase in strain. The disruption processes appear to be triggered by the existence of adjacent ductile polyolefins and their micromechanical deformations. The degree of nanopore development significantly varies among blends and layered systems, depending on several material parameters, such as the interfacial strength. Discrete nanopores are observed more often in metallocene systems and blends than in Ziegler–Natta systems and layered films. Macroscopically, with the development of discrete nanopores, metallocene systems show higher strain to break and significant shear yielding under tension. The disruption mechanism can be used to prepare nanoporous materials or to improve the mechanical performances of polyolefins. © 2004 Wiley Periodicals, Inc. J Appl Polym Sci 95: 708–718, 2005 相似文献
169.
Russell RB; Saqi MA; Bates PA; Sayle RA; Sternberg MJ 《Protein engineering, design & selection : PEDS》1998,11(1):1-9
Fold recognition methods aim to use the information in the known protein
structures (the targets) to identify that the sequence of a protein of
unknown structure (the probe) will adopt a known fold. This paper
highlights that the structural similarities sought by these methods can be
divided into two types: remote homologues and analogues. Homologues are the
result of divergent evolution and often share a common function. We define
remote homologues as those that are not easily detectable by sequence
comparison methods alone. Analogues do not have a common ancestor and
generally do not have a common function. Several sets of empirical matrices
for residue substitution, secondary structure conservation and residue
accessibility conservation have previously been derived from aligned pairs
of remote homologues and analogues (Russell et al., J. Mol. Biol., 1997,
269, 423-439). Here a method for fold recognition, FOLDFIT, is introduced
that uses these matrices to match the sequences, secondary structures and
residue accessibilities of the probe and target. The approach is evaluated
on distinct datasets of analogous and remotely homologous folds. The
accuracy of FOLDFIT with the different matrices on the two datasets is
contrasted to results from another fold recognition method (THREADER) and
to searches using mutation matrices in the absence of any structural
information. FOLDFIT identifies at top rank 12 out of 18 remotely
homologous folds and five out of nine analogous folds. The average
alignment accuracies for residue and secondary structure equivalencing are
much higher for homologous folds (residue approximately 42%, secondary
structure approximately 78%) than for analogues folds (approximately 12%,
approximately 47%). Sequence searches alone can be successful for several
homologues in the testing sets but nearly always fail for the analogues.
These results suggest that the recognition of analogous and remotely
homologous folds should be assessed separately. This study has implications
for the development and comparative evaluation of fold recognition
algorithms.
相似文献
170.