The effects of cyclic fatty acid monomers on cultured porcine endothelial cells

The popularity of polyunsaturated oils used in food applications and preparation continues to appreciate as a result of positive health claims. With polyunsaturated oils inherently more susceptible to oxidative and thermal degradation, the formation of new fatty acid species increases considerably. The presence of one species known as cyclic fatty acid monomers (CFAM) has been detected as a component of many oils subjected to various thermal processes including deep-fat frying. The effect of CFAM on metabolic processes has not been fully characterized. In this study, confluent porcine aortic endothelial cells incorporated CFAM into their polar and nonpolar lipid fractions following a 48-h exposure to 31 and 62 ppm CFAM in the culture medium. Subsequently, the influence of CFAM incorporation on various membrane-dependent physical properties and biochemical processes was investigated. CFAM decreased the lipid packing order of the membrane bilayer core but did not alter the lipid packing order of lipid chain segments at or near the lipid-water interface of the membrane. CFAM led to significant reductions in Ca²⁺ ATPase activity and monolayer integrity while eliciting a significant increase of prostacyclin synthesis and secretion.     

Fiber Suspensions in Complex Geometries: Flow/Orientation Coupling

A Galerkin finite element solution is developed for the flow of fiber suspensions. Primary variables are velocity, pressure, and a second‐order tensor describing the fiber orientation. The model treats the orientation as three‐dimensional, includes fiber—fiber interaction effects, and uses an orthotropic closure approximation. The flow and orientation are strongly coupled through an orientation‐dependent constitutive equation. We explore the effect of this coupling on the fluid mechanics of fiber suspensions by studying three flows: an axisymmetric contraction, an axisymmetric expansion, and a center‐gated disk. Coupling enhances the corner vortex in the contraction, in quantitative agreement with published experiments and calculations. The expansion results demonstrate that the aligned‐fiber approximation is not valid for this flow. In the center‐gated disk the effects of coupling are modest and are only noticeable near the center of the disk. This supports the use of decoupled models for injection molding in thin cavities.     

Absolute calibration method for nanosecond-resolved, time-streaked, fiber optic light collection, spectroscopy systems

This paper describes a convenient and accurate method to calibrate fast (<1 ns resolution) streaked, fiber optic light collection, spectroscopy systems. Such systems are inherently difficult to calibrate due to the lack of sufficiently intense, calibrated light sources. Such a system is used to collect spectral data on plasmas generated in electron beam diodes fielded on the RITS-6 accelerator (8-12MV, 140-200kA) at Sandia National Laboratories. On RITS, plasma light is collected through a small diameter (200 μm) optical fiber and recorded on a fast streak camera at the output of a 1 meter Czerny-Turner monochromator. For this paper, a 300 W xenon short arc lamp (Oriel Model 6258) was used as the calibration source. Since the radiance of the xenon arc varies from cathode to anode, just the area around the tip of the cathode ("hotspot") was imaged onto the fiber, to produce the highest intensity output. To compensate for chromatic aberrations, the signal was optimized at each wavelength measured. Output power was measured using 10 nm bandpass interference filters and a calibrated photodetector. These measurements give power at discrete wavelengths across the spectrum, and when linearly interpolated, provide a calibration curve for the lamp. The shape of the spectrum is determined by the collective response of the optics, monochromator, and streak tube across the spectral region of interest. The ratio of the spectral curve to the measured bandpass filter curve at each wavelength produces a correction factor (Q) curve. This curve is then applied to the experimental data and the resultant spectra are given in absolute intensity units (photons∕sec∕cm(2)∕steradian∕nm). Error analysis shows this method to be accurate to within +∕- 20%, which represents a high level of accuracy for this type of measurement.     

Model-based predictive control of office window shades

In the automation of interior window shading devices, a control system that relies on a prediction of environmental conditions and a building's thermal response can provide savings to space-conditioning loads beyond what can be achieved using a reactive approach. The development of these control strategies can be difficult because of the uniqueness of each building. A simplified model-based predictive control (MPC) method for window shades is proposed. To this end, a control-oriented model representing the heat transfer problem in a perimeter office space was developed. The parameters of the model were estimated using the ensemble Kalman filter (EnKF). The energy-savings potential of the EnKF-based MPC approach for window shades was investigated using EnergyPlus simulations. This was accomplished by implementing the control-oriented model into the energy management system application of EnergyPlus. Simulations were conducted to assess the energy saving potential of using the EnKF-based MPC for roller blinds in a south-facing perimeter office space in Ottawa, Canada. The simulation-based results indicate the potential for about 35% reduction in electricity usage for space conditioning over manually operated interior roller blinds.     

Moderating effects of three coping strategies and self-esteem on perceived discrimination and depressive symptoms: A minority stress model for Asian international students.

This study examined 3 coping strategies (reflective, suppressive, and reactive), along with self-esteem, as moderators of the relation between perceived discrimination and depressive symptoms. International students (N = 354) from China, India, Korea, Taiwan, and Hong Kong provided data via an online survey. The role of perceived general stress was statistically controlled. Hierarchical regression analyses indicated a significant direct effect of perceived discrimination, a significant 2-way interaction of perceived discrimination and suppressive coping, and a significant 3-way interaction of perceived discrimination, reactive coping, and self-esteem in predicting depressive symptoms. An increased tendency to use suppressive coping appeared to strengthen the association between perceived discrimination and depressive symptoms. In contrast, the association between perceived discrimination and depressive symptoms was not significant when reactive coping was infrequently used, but only for students with relatively high self-esteem. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Approximated Fractional-Order Inverse Chebyshev Lowpass Filters

In this paper we use a least-squares fitting routine to approximate the stopband ripple characteristics of fractional-order inverse Chebyshev lowpass filters which have fractional-order zeros and poles. MATLAB simulations of \((1+\alpha )\)-order lowpass filters with fractional steps from \(\alpha =0.1\) to \(\alpha =0.9\) are given as examples. SPICE simulations of 1.2-, 1.5-, and 1.8-order lowpass filters and experimental results of a 1.5-order filter using approximated fractional-order capacitors in a Multiple-Input Biquad circuit validate the implementation of these circuits.     

Microfluidic purification and preconcentration of mRNA by flow-through polymeric monolith

Efficient and rapid isolation of mRNA is important in the field of genomics as well as in the clinical and pharmaceutical arena. We have developed UV-initiated methacrylate-based porous polymer monoliths (PPM) for microfluidic trapping and concentration of eukaryotic mRNA. PPM are cast-to-shape and are tunable for functionalization using a variety of amine-terminated molecules. Efficient isolation of eukaryotic mRNA from total RNA was first mathematically modeled and then achieved using PPM in capillaries. Purification protocols using oligo dT's, locked nucleic acid substituted dT's, and tetramethylammonium chloride salts were characterized. mRNA yield and purity were compared with mRNA isolated by commercial kits with statistically equivalent yields and purities (determined by qPCR ratio of 18s rRNA and Gusb mRNA markers). Even after extracting 16 microg of mRNA from 315 microg of total RNA, the 0.4-microL volume monolith showed no signs of saturation. Elution volumes were below 20 microL with concentrations up to 1 microg/microL. In addition, the polymeric material exhibited exceptional stability in a range of conditions (pH, temperature, dryness) and was stable for a period of months. All of these characteristics make porous polymer monoliths good candidates for potential microfluidic sample preconcentrators and purifiers.     

Two-wave-plate compensator method for full-field retardation measurements

The two-wave-plate compensator (TWC) method is expanded for full-field retardation measurements by use of a polarization microscope. The sample image is projected onto a CCD camera connected to a computer, allowing the retardation to be measured at all pixels. The retardation accuracy of this implementation of the TWC is evaluated to be 0.06 nm. The method is applied to polarization-maintaining fibers and long-period fiber gratings. The measured retardation is in good agreement with the crossed-polarizer images of the fibers. The method achieves a spatial resolution of 0.45 microm and a retardation resolution of 0.07 nm. The full-field TWC method can thus be a useful tool for characterizing and monitoring the fabrication of optical devices.     

Bulk acoustic resonator based on piezoelectric ZnO belts

In this paper, a bulk acoustic resonator based on ZnO belts is demonstrated. This device shows a great deal of promise in applications as an electronic filter and as a mass sensor. The fabricated device was characterized using vector network analysis, and both the first and third harmonics of resonance were observed at approximately 247 and 754 MHz, respectively. A one-dimensional Krimholt-Leedom-Matthaei model was utilized to predict the resonant frequency of the device and confirm the observed behavior.