Thymic dendritic cells are primary targets for the oncogenic virus SL3-3

The murine retrovirus SL3-3 causes malignant transformation of thymocytes and thymic lymphoma in mice of the AKR and NFS strains when they are inoculated neonatally. The objective of the present study was to identify the primary target cells for the virus in the thymuses of these mice. Immunohistochemical studies of the thymus after neonatal inoculation of the SL3-3 virus showed that cells expressing the viral envelope glycoprotein (gp70(+) cells) were first seen at 2 weeks of age. These virus-expressing cells were found in the cortex and at the corticomedullary junction in both mouse strains. The gp70(+) cells had the morphology and immunophenotype of dendritic cells. They lacked macrophage-specific antigens. Cell separation studies showed that bright gp70(+) cells were detected in a fraction enriched for dendritic cells. At 3 weeks of age, macrophages also expressed gp70. At that time, both gp70(+) dendritic cells and macrophages were found at the corticomedullary junction and in foci in the thymic cortex. At no time during this 3-week period was the virus expressed in cortical and medullary epithelial cells or in thymic lymphoid cells. Infectious cell center assays indicated that cells expressing infectious virus were present in small numbers at 2 weeks after inoculation but increased at 5 weeks of age by several orders of magnitude, indicating virus spread to the thymic lymphoid cells. Thus, at 2 weeks after neonatal inoculation of SL3-3, thymic dendritic cells are the first cells to express the virus. At 3 weeks of age, macrophages also express the virus. In subsequent weeks, the virus spreads to the thymocytes. This pathway of virus expression in the thymus allows the inevitable provirus integration in a thymocyte that results in a clonal lymphoma.     

Human immunodeficiency virus inhibits multilineage hematopoiesis in vivo

Human immunodeficiency virus type 1 (HIV-1)-infected individuals often exhibit multiple hematopoietic abnormalities reaching far beyond loss of CD4(+) lymphocytes. We used the SCID-hu (Thy/Liv) mouse (severe combined immunodeficient mouse transplanted with human fetal thymus and liver tissues), which provides an in vivo system whereby human pluripotent hematopoietic progenitor cells can be maintained and undergo T-lymphoid differentiation and wherein HIV-1 infection causes severe depletion of CD4-bearing human thymocytes. Herein we show that HIV-1 infection rapidly and severely decreases the ex vivo recovery of human progenitor cells capable of differentiation into both erythroid and myeloid lineages. However, the total CD34+ cell population is not depleted. Combination antiretroviral therapy administered well after loss of multilineage progenitor activity reverses this inhibitory effect, establishing a causal role of viral replication. Taken together, our results suggest that pluripotent stem cells are not killed by HIV-1; rather, a later stage important in both myeloid and erythroid differentiation is affected. In addition, a primary virus isolated from a patient exhibiting multiple hematopoietic abnormalities preferentially depleted myeloid and erythroid colony-forming activity rather than CD4-bearing thymocytes in this system. Thus, HIV-1 infection perturbs multiple hematopoietic lineages in vivo, which may explain the many hematopoietic defects found in infected patients.     

Pattern recognition: An alternative to parameter identification in adaptive control

Practical models used in identification of process control processes must be too simplistic to give precise control information. However, these models can be used for adaptation if they are continuously readapted. But the identification then lacks the precision which might justify the analytic elaboration. One alternative has been to use pattern recognition as a means for allowing a computer system to characterize transient response computing readapted parameters which cause the control behavior to approach a desired transient ‘shape’. The paper summarizes work using pattern features as a basis for practice and theory.     

Aμ-MAC: 一种自适应的无线传感器网络MAC协议

结合基于竞争和调度机制的混合型方案是高效的无线传感器网络MAC协议的重要解决思路. μ-MAC是一种典型的混合型MAC协议, 本文在深入研究μ-MAC的基础上, 提出一种自适应的混合型协议Aμ-MAC. 它针对动态流量的数据采集型应用, 解决了μ-MAC中动态拓扑适应性及时钟同步问题, 并提供了良好的流量自适应性. 仿真结果表明, Aμ-MAC增强了协议的扩展性和适应性, 具有更好的网络生存能力, 而保留了与μ-MAC相近的节能效率和时延性能.     

On the Right Track: Systematic Implementation of Ergonomics in Railway Network Control

At a time of change for the railway networks of Europe we have been developing tools to assess ergonomics aspects of railway network control. This is within the Railway Ergonomics Control Assessment Package (RECAP). Among the developments have been an audit instrument (REQUEST), tools to assess situation awareness (RESA) and staff loading (RELOAD), and workshops to predict opportunities for human error and organisational failure across rail network operations. This research is discussed with respect to the context of UK railway operations and the need for an expanding tradition of cognitive ergonomics fieldwork. From the findings we draw some conclusions about the roles filled by signallers, electrical controllers and zone controllers within a perspective of distributed cognitive/social networks.     

Measuring bovine mammary gland blood flow using a transit time ultrasonic flow probe

Lactating cattle were used to validate a transit time ultrasonic blood flow metering system for measuring mammary gland arterial blood flow. Blood flow probes were surgically placed around the right external pudic artery. An electromagnetic flow probe was implanted in tandem with the ultrasonic probe in two cows for comparative measurements. The absolute accuracy of the implanted flow probes was assessed in vivo by mechanical means on anesthetized cows after 2 to 3 wk of implantation. The zero offset of the ultrasonic probes ranged from -12 to 8 ml/min. When the ultrasonic probe was properly implanted, the slopes of the calibration curves were linear and ranged from .92 to .95, tracking absolute flow to within 8%. The transit time instrument's performance was examined under a variety of physiological conditions. These included milking and hormone injections. The transit time ultrasonic flow meter accurately measured physiological changes in mammary arterial blood flow in chronically prepared conscious cattle. Blood flow increased 29% during milking. Epinephrine decreased mammary blood flow by 90 to 95%. Oxytocin doses increased mammary blood flow by 15 to 24%.