Influence of the phase diagram on the diffuse interface thickness and on the microstructure formation in a phase-field model for binary alloy

A phase-field model is used to investigate the responses of planar interfaces and of eutectic microstructures on the different shapes of the phase diagram in binary alloy systems. Numerical solutions of the dynamic field equations show that the interfacial profile and the thickness of the diffuse boundary layer depend on the segregation of the alloy components. The simulations are presented for different openings of binary phase diagrams. A strong influence of the phase diagram on the evolution of eutectic microstructures is found and quantitatively evaluated by defining an appropriate measure. The results are interpreted in terms of weighting the different contributions in the phase-field equation.     

Paternal Uniparental Isodisomy of Chromosome 2 in a Patient with CNGA3-Associated Autosomal Recessive Achromatopsia

Achromatopsia (ACHM) is a rare autosomal recessively inherited retinal disease characterized by congenital photophobia, nystagmus, low visual acuity, and absence of color vision. ACHM is genetically heterogeneous and can be caused by biallelic mutations in the genes CNGA3, CNGB3, GNAT2, PDE6C, PDE6H, or ATF6. We undertook molecular genetic analysis in a single female patient with a clinical diagnosis of ACHM and identified the homozygous variant c.778G>C;p.(D260H) in the CNGA3 gene. While segregation analysis in the father, as expected, identified the CNGA3 variant in a heterozygous state, it could not be displayed in the mother. Microsatellite marker analysis provided evidence that the homozygosity of the CNGA3 variant is due to partial or complete paternal uniparental isodisomy (UPD) of chromosome 2 in the patient. Apart from the ACHM phenotype, the patient was clinically unsuspicious and healthy. This is one of few examples proving UPD as the underlying mechanism for the clinical manifestation of a recessive mutation in a patient with inherited retinal disease. It also highlights the importance of segregation analysis in both parents of a given patient or especially in cases of homozygous recessive mutations, as UPD has significant implications for genetic counseling with a very low recurrence risk assessment in such families.     

Discrimination of vegetation strata in a multi-layered Mediterranean forest ecosystem using height and intensity information derived from airborne laser scanning

Height and intensity information derived from Airborne Laser Scanning (ALS) was used to obtain a quantitative vertical stratification of vegetation in a multi-layered Mediterranean ecosystem. A new methodology for the separation of different vegetation strata was implemented using supervised classification of a two-dimensional feature space spanned by ALS return height (terrain corrected) and intensity. The classification was carried out using Gaussian mixture models tuned on a control plot. The approach was validated using extensive field measurements from treated plots, ranging from single vegetation strata to a more complex multi-layered ecosystem. Plot-level canopy profiles derived from ALS and from a geometric reconstruction based on field measurements were in very good agreement, with correlation coefficients ranging from 0.73 (for complex, 3-layered) to 0.96 (simple, single-layered). In addition, it was possible to derive plot-level information on layer height, vertical extent and coverage with absolute accuracies of some decimetres (simple plots) to a meter (complex plots) for both height and vertical extent and about 10 to 15% for layer coverage. The approach was then used to derive maps of the layer height, vertical extent and percentage of ground cover for a larger area, and classification accuracy was evaluated on a per-pixel basis. The method performed best for single-layered plots or dominant layers on multi-layered plots, obtaining an overall accuracy of 80 to 90%. For subdominant layers in the more complex plots, accuracies obtained were as low as 48%.Our results demonstrate the possibility of deriving qualitative (presence and absence of specific vegetation layers) and quantitative, physical data (height, vertical extent and ground cover) describing the vertical structure of complex multi-layered forest ecosystems using ALS-based height and intensity information.     

Molecular Fingerprint Recombination: Generating Hybrid Fingerprints for Similarity Searching from Different Fingerprint Types

Molecular fingerprints have a long history in computational medicinal chemistry and continue to be popular tools for similarity searching. Over the years, a variety of fingerprint types have been introduced. We report an approach to identify preferred bit subsets in fingerprints of different design and “recombine” these bit segments into “hybrid fingerprints”. These compound class‐directed fingerprint representations are found to increase the similarity search performance of their parental fingerprints, which can be rationalized by the often complementary nature of distinct fingerprint features.     

Levels of PCBs and their metabolites in the serum of residents of a highly contaminated area in eastern Slovakia

The over-riding aim of the present investigation was to obtain information concerning exposure that can be used as a basis for studies on the health of individuals residing in the Michalovce area of eastern Slovakia which is heavily contaminated by polychlorinated biphenyls (PCBs). Accordingly, this work focused on determination of serum concentrations of hydroxylated (OH-PCBs) and methylsulfonyl-substituted (MeSO2-PCBs) metabolites of PCBs. One hundred and twenty-two men and women, 20-59 years of age, living in the contaminated area and 175 from the control Stropkov/Svidnik district were selected randomly from 2047 sampled individuals. Following a specially designed cleanup, the levels of various congeners of OH-PCBs and MeSO2-PCBs in their serum were quantitated by gas chromatography and mass spectrometry, with comparison to authentic reference standards. The median concentrations of PCB congeners and their OH-PCB and MeSO2-PCB metabolites were 2-3-fold higher in residents of Michalovce than in the control region. The levels of certain OH-PCB metabolites were in the same high range as those of individual PCB congeners, whereas the MeSO2-PCB levels were significantly lower. The PCB and their metabolites were present at slightly higher concentrations in men than in women, and the serum levels of PCBs and MeSO2-PCBs increased with increasing age. Thus, the environmental contamination resulting from previous industrial production of PCBs has led to elevated concentrations of PCBs and their metabolites in the serum of individuals living in the Michalovce area.     

Correlation between visual defects and increased dark current in large-area Hg1−xCdxTe photodiodes

The Cross-Track Infrared Sounder (CrIS) program [an instrument on the National Polar-Orbiting Operational Environmental Satellite System (NPOESS)] requires photodiodes with spectral cutoffs denoted by short-wavelength infrared [γ_c(98 K) ∼5.1 μm], midwavelength infrared [γ_c(98 K) ∼9.1 μm], and long-wavelength infrared (LWIR) [γ_c(81 K) ∼15.5 μm]. The CrIS instrument also requires large-area (850-μm-diameter) photodiodes with state-of-art performance. Molecular beam epitaxy (MBE) is used to grow n-type short-wavelength infrared, midwavelength infrared, or LWIR Hg_1−xCd_xTe on latticematched CdZnTe. Detectors with p-type implants 7 μm in diameter are used to constitute the 850-μm-diameter lateral collection diodes (LCDs). The photodiode architecture is the double-layer planar heterostructure architecture. Quantum efficiency, I-V, R_d-V, and 1/f noise in photovoltaic Hg_1−xCd_xTe detectors are critical parameters that limit the sensitivity of infrared sounders. These are some of the parameters used to select photodiodes that will be part of the CrIS focal plane module (FPM). During fabrication of the FPM, the photodiodes are subject to a significant amount of handling while transitioning from part of newly processed Hg_1–xCd_xTe wafers to individual photodiodes mounted in a CrIS FPM ready to be flown on NPOESS. Quantum efficiency, I-V, noise, and visual inspections are performed at several steps in the detector’s journey. Initial I-V and visual inspections are conducted at the wafer level followed by I-V, noise, and quantum efficiency after dicing and mounting the photodiodes in leadless chip carriers (LCCs). A visual inspection is performed following removal of the detectors from the LCCs. Finally, the individual photodiodes are precision mounted on an FPM base, and I-V, noise, quantum efficiency, and visual inspections are performed again. Each step in the FPM fabrication process requires handling and environmental conditioning that can result in detector dark current and noise increase. Some photodiodes on the first flightlike FPMs fabricated exhibited an increase in dark current and noise characteristics at the FPM level as compared to the measurements performed when the photodiodes were in LCCs prior to integration into the FPM. The degradation observed resulted in an investigation to discern the cause of the performance degradation (baking at elevated temperatures, mechanical handling, electrical stress, etc.). This paper outlines the results of the study and the corrective actions that led to the successful manufacture of LWIR large detectors from material growth to insertion into flight FPMs for the CrIS program.