Gene amplification in Chinese hamster DNA repair deficient mutants

In order to study the possible relationship between gene amplification and DNA repair we analyzed the amplification of the CAD gene in four mutants hypersensitive to UV light (CHO43RO, CHO7PV, UV5 and UV61) isolated in vitro from Chinese hamster cell lines (CHO-K1 and AA8). These mutants are characterized by different defects in the nucleotide excision repair mechanism and represent complementation groups 1, 9, 2, and 6 respectively. To evaluate the amplification ability of each cell line we measured the rate of appearance of PALA resistant clones with the Luria and Delbrück fluctuation test. Resistance to PALA is mainly due to amplification of the CAD gene. In the mutants CHO43RO, UV5 and CHO7PV we reproducibly found an amplification rate lower than in the parental cell lines (2-5 times), while in UV61 the amplification rate was about 4 times higher. This result indicates that each mutant is characterized by a specific amplification ability and that the unefficient removal of UV induced DNA damage can be associated with either a higher or a lower amplification rate. However, the analysis of randomly isolated CHO-K1 clones with normal UV sensitivity has shown variability in their amplification ability, making it difficult to relate the specific amplification ability of the mutants to the DNA repair defect and suggesting clonal heterogeneity of the parental population.     

A methodology for the performance evaluation of data querystrategies in universal mobile telecommunication systems (UMTS)

The anticipated emergence of third-generation mobile systems, referred to as universal mobile telecommunication systems (UMTS), raises the problem of reconsidering the design of the databases destined to contain the user information. In particular, it is expected that the key concepts of the new database architectures will be high distribution and fast updating of information. So far, the problem of determining the most appropriate distributed database (DDB) architectures for third-generation mobile systems has not been widely dealt with in literature. This paper presents a methodology for evaluating hierarchical DDB architectures by means of an analytical model of the data querying operation. This methodology allows for structural alternatives, differing on account of the number of levels and branches in the hierarchy, to be evaluated in terms of query loads and mean response times, according to a given user mobility characterization and a given search protocol operation. By way of illustration, the paper discusses a case study, concerning a query operation arising from a location updating procedure and applied to a hierarchical tree-like DDB in which some structural alternatives are considered     

Electronic properties of CuO2 planes

We study a 3-band model of CuO₂ with “bare bones” interactions: hopping from copper ions to nearest-neighbor oxygenst _pd only, a two-body interaction on the copper ionsU _dd only, and an overlap copper-oxygen parameter λ_pd. In the limitt _pd ∝U _dd ^1/2→∞, t^*=t _pd ²/2U _dd is the unit of energy with 8λ_pd ² as the only parameter of significance. If the two-body interaction is invariant under particle-hole interchange, the low-lying states (energy O(t^*)) can be described by conserved particles and can all be classified. They are quite distinct from the high-lying states (energies O(U _dd)). The dynamics of the conserved fermion-like elementary particles are well described by a modifiedt-J model with extended hopping and nearest-neighbor superexchange attraction. This is a scenario known to be favorable to high-temperature superconductivity, but it must be noted that both the hopping range and the exchange are functions of 8λ_pd ². Moreover, if the Hamiltonian isnot invariant under particle-hole interchange the dynamics becomes much more complex and possibly more inimical to high-temperature superconductivity. This may provide an explanation for the deleterious effect on superconductivity of very small concentrations of certain impurities.     

Treatment of perianal fistulas with ND:YAG laser--results in twenty cases

Excision of perianal fistulas using a 1.064 micron wavelength neodymium:yttrium aluminum garnet (ND:YAG) contact tipped laser with primary wound closure was used to treat 20 dogs with perianal fistulas. Overall, 19 of 20 (95%) dogs had resolution of fistulas after one or more ND:YAG treatments. The period of resolution ranged from 10 to 42 months with a mean of 22.9 months. Sixteen of 20 (80%) dogs had resolved fistulas after one laser excision. Three of the four recurrences underwent additional laser treatments with successful results. The total number of laser procedures ranged from one to three with a mean of 1.2 procedures. Postoperatively, anal tone as judged by digital rectal examination was reduced in about 60% of the cases, but clinical evidence of fecal incontinence only occurred in four of 20 cases. This was managed effectively with diet modification. The tendency toward loss of anal tone or fecal incontinence depended on the severity of preexisting anal stenosis. On a client survey, 19 of 20 owners believed that their animals experienced less pain during defecation after surgery and rated the results as excellent or good. The overall success rate using ND:YAG laser excision compared very favorably with previously reported studies of other methods of treatment for perianal fistulas in dogs.     

Nickel- and copper-coated carbon fibre reinforced tin-lead alloy composites

Nickel and copper were deposited over brominated, surface treated, and pristine P-100 carbon fibres using cementation and electroplating techniques. The fibres were brominated by bromine vapour for 48 h and then desorbed at 200 °C in air for 12 h. The anodic oxidation treatment of the fibres involved electrochemical etching in a dilute sodium hydroxide electrolyte for 3 min. Electroplated coated fibres showed better tensile properties than cementation coated fibres. In addition, nickel coating exhibited better bonding with the carbon fibres compared to copper coating. The effect of bromination and surface treatment was improved adhesion between coating and fibres. Nickel- and copper-coated fibres, which were brominated, anodically oxidized, and pristine, reinforced tin-lead alloy composites were fabricated by squeeze casting. The composites containing coated treated carbon fibres had higher tensile and shear strength than the ones containing coated pristine carbon fibres. Moreover, the composite with coated brominated carbon fibres had better tensile strength and shear strength than the surface treatment. The results also showed the composites containing nickel-coated fibres had higher tensile and shear strength than the ones containing copper-coated fibres.     

The material properties of sulphonated phenolic resin reinforced cement mortars

The material properties of new sulphonated phenolic resin (SP) reinforced cement mortars have been investigated. SP was found to promote the dispersion of cement particles and to interact with Ca(OH)₂. As a result, the resulting mortars exhibit better workability, more compact structure and higher compressive strength than plain mortars. The mortar with 1 wt% SP present after 28 days curing exhibits a compressive strength of 66MPa, which is about 18% higher than that of plain mortar.     

Transurethral ultrasound array for prostate thermal therapy:initial studies

This study presents the initial evaluation of an applicator designed for transurethral ultrasound thermotherapy (TUST) of prostate tissue in the treatment of benign prostatic hyperplasia (BPH) and cancer. A tubular multitransducer applicator, consisting of four piezoceramic tubes (2.5 mm diameter, 6 mm long, 6.8 MHz) under separate power control, was designed to fit within a semiflexible water-cooled temperature-regulated delivery catheter to be placed within the prostatic urethra during therapy. Sonication patterns were tailored to produce power depositions which avoid nontargeted tissues, such as the rectum. Computer simulations have demonstrated that 1.4-2.0 cm radial therapeutic zones (temperatures ⩾50-55°C, thermal doses >300 EM₄₃) with concurrent sparing of the urethral mucosa can be produced within prostate tissue having blood perfusion as high as 10 kg m^-3 s^-1 within 15-30 min. Acoustic distributions and power output measurements of a prototype applicator have demonstrated acoustic power levels approaching 10 W per each sectored transducer segment are attainable, with beam profiles collimated within the transducer length and with desired circumferential distributions. In vivo thermal dosimetry characterizations of these transurethral applicators have indicated that therapeutic temperatures between 50 and 90°C are attainable, controllable in the longitudinal and circumferential directions, and have effective radial heating. These results clearly indicate that transurethral ultrasound applicators have potential to provide improved spatial localization and control of the heating distribution over existing transurethral thermal therapy techniques for both hyperthermia and thermal coagulative therapy of the prostate