Pressures and procedures for the design of safe consumer products

An assessment is provided of the current position regarding changes in UK or EEC civil law under which injured parties can obtain redress in respect of injuries caused by defective products. One effect of proposed systems of strict liability in tort will be to shift some of the burden of proof from the injured party on to the producer of the product claimed to have caused injury. It is argued that this is a just and logical change, given the unfairness and anomalies in present systems and the relative abilities of consumers and producers to avoid such incidents and to bear or to offset the cost of the consequences. Cases of apparent misuse of a product by a consumer may frequently be induced by the design of that product and could thus be prevented by more thoughtful design. In order that producers can ensure their own viability and satisfy the consumer market, their products must be safe, efficient, comfortable and satisfying to use, as well as durable, serviceable and realistically priced. One requirement for achieving this has been said to be the input of accident and ergonomics data early in the product development process; the present paper examines this proposition. Evidence is provided by research into the design and manufacture of consumer products for the UK market, carried out at Birmingham University. Examined in particular are the extent to which formal development processes and design safety review procedures are used, and the degree of utilisation of accident, ergonomics, user testing and market feedback data, identifying barriers to the widespread use of these. Safe and ergonomic design of products must aim to be seen as a positive business activity, rather than as a cost-centre. The benefits to a company of a comprehensive design safety programme will more than outweigh the costs given the pressures identified earlier.     

Double-pass oblique-incidence interferometer for the inspection of nonoptical surfaces

Interferometric testing of the flatness or straightness of ground surfaces of metals, granite, ceramics, and glasses has always proved difficult because of the extremely low specular reflection from these optically rough surfaces. At oblique angles of incidence the specular reflection coefficient of nonoptical surfaces increases rapidly, typically from near 0.01 at 75 degrees up to, say, 0.50 at 85 degrees and near 1.00 at 90 degrees angle of incidence. By taking advantage of this enhanced reflection at oblique incidence and the brightness and spatial coherence of visible gas lasers, it becomes practical to use interferometric inspection of nonoptical surfaces. A novel double-pass oblique-incidence interferometer is described which enables testing of large pieces having a specular reflection as low as 1%.     

Analysing quadratic effects of formative constructs by means of variance-based structural equation modelling

Together with the development of information systems research, there has also been increased interest in non-linear relationships between focal constructs. This article presents six Partial Least Squares-based approaches for estimating formative constructs’ quadratic effects. In addition, these approaches’ performance is tested by means of a complex Monte Carlo experiment. The experiment reveals significant and substantial differences between the approaches. In general, the performance of the hybrid approach as suggested by Wold (1982) is most convincing in terms of point estimate accuracy, statistical power, and prediction accuracy. The two-stage approach suggested by Chin et al (1996) showed almost the same performance; differences between it and the hybrid approach – although statistically significant – were unsubstantial. Based on these results, the article provides guidelines for the analysis of non-linear effects by means of variance-based structural equation modelling.     

Serial measurements of body composition in obese subjects during a very-low-energy diet (VLED) comparing bioelectrical impedance with hydrodensitometry

Bioelectrical impedance analysis (BIA) is a convenient, inexpensive, and noninvasive technique for measuring body composition. BIA has been strongly correlated with total body water (TBW) and also has been validated against hydrodensitometry (HD). The accuracy and clinical utility of BIA and HD during periods of substantial weight loss remain controversial. We measured body composition in moderately and severely obese patients serially using both methods during a very-low-energy diet (VLED). Mean initial weight in these patients was 116 (+/-30) kg (range, 74-196 kg). Mean weight loss was 24 (+/-13) kg with a decrease in fat mass (FM) by HD of kg (p < 0.001) and a decrease in fat-free mass (FFM) of 3.6 kg (p < 0.05). Loss of FFM is best predicted by the rate (kg/wk) of weight loss (r2 = 0.86, p < 0.0001). FFM, as predicted from BIA equations, was highly correlated with FFM as estimated by HD during all testing sessions (r = 0.92-0.98). Although highly correlated, BIA overestimated FFM relative to HD and this difference appeared to be more pronounced for taller patients with greater truncal obesity. Although the discrepancy was no greater during weight-loss treatment, the level of disagreement was considerable. Therefore, the two methods cannot be used interchangeably to monitor relative changes in body composition in patients with obesity during treatment with VLED. The discrepancy between BIA and HD may be caused by body mass distribution considerations and by perturbations in TBW which affect the hydration quotient for FFM (BIA) and/or which affect the density constants for FFM and FM (HD).     

RBS analysis of front contacts for surface-barrier detectors

Surface-barrier detector structures based on Au, WTi and Au/WTi contacts on (111) n-Si were analysed. The metal layers were deposited by evaporation or sputtering to 40–100 nm thicknesses. The aim was to improve the thermal stability of the surface-barrier structures and retain the favourable detector properties of the Au/Si system. Contact structures which contain WTi layers have shown a much better thermal stability compared to the Au/Si system, but the properties of the detectors were poorer. The probable reasons are the reactivity of W and Ti with the thin oxide layer on the silicon surface and perhaps the higher energy of adatoms during sputter-deposition. Both effects can influence the degradation of the surface-barrier properties.     

Tumor-Localized Administration of α-GalCer to Recruit Invariant Natural Killer T Cells and Enhance Their Antitumor Activity against Solid Tumors

Invariant natural killer T (iNKT) cells have the capacity to mount potent anti-tumor reactivity and have therefore become a focus in the development of cell-based immunotherapy. iNKT cells attack tumor cells using multiple mechanisms with a high efficacy; however, their clinical application has been limited because of their low numbers in cancer patients and difficulties in infiltrating solid tumors. In this study, we aimed to overcome these critical limitations by using α-GalCer, a synthetic glycolipid ligand specifically activating iNKT cells, to recruit iNKT to solid tumors. By adoptively transferring human iNKT cells into tumor-bearing humanized NSG mice and administering a single dose of tumor-localized α-GalCer, we demonstrated the rapid recruitment of human iNKT cells into solid tumors in as little as one day and a significantly enhanced tumor killing ability. Using firefly luciferase-labeled iNKT cells, we monitored the tissue biodistribution and pharmacokinetics/pharmacodynamics (PK/PD) of human iNKT cells in tumor-bearing NSG mice. Collectively, these preclinical studies demonstrate the promise of an αGC-driven iNKT cell-based immunotherapy to target solid tumors with higher efficacy and precision.     

AlphaFold2: A Role for Disordered Protein/Region Prediction?

The development of AlphaFold2 marked a paradigm-shift in the structural biology community. Herein, we assess the ability of AlphaFold2 to predict disordered regions against traditional sequence-based disorder predictors. We find that AlphaFold2 performs well at discriminating disordered regions, but also note that the disorder predictor one constructs from an AlphaFold2 structure determines accuracy. In particular, a naïve, but non-trivial assumption that residues assigned to helices, strands, and H-bond stabilized turns are likely ordered and all other residues are disordered results in a dramatic overestimation in disorder; conversely, the predicted local distance difference test (pLDDT) provides an excellent measure of residue-wise disorder. Furthermore, by employing molecular dynamics (MD) simulations, we note an interesting relationship between the pLDDT and secondary structure, that may explain our observations and suggests a broader application of the pLDDT for characterizing the local dynamics of intrinsically disordered proteins and regions (IDPs/IDRs).     

Study of the adhesion of Staphylococcus aureus to coated glass substrates

The adhesion of Staphylococcus aureus was studied using a selection of laboratory-prepared and commercially available coated glass substrates using a simple methodology. Substrates were examined by scanning electron microscopy, atomic force microscopy and water droplet contact angles. It was found that microbial adhesion was independent of surface roughness, when this was of a lower magnitude than microbial size. It was also found that microbial adhesion was greater for hydrophilic surfaces than for hydrophobic ones, but that on a photoinduced superhydrophilic surface, microbes were more spread out—a potential benefit for more effective photocatalytic disinfection. It is suggested that hydrophobic and photoinduced superhydrophilic surface coatings both have potential as a means of reducing microbial fouling of surfaces.     

Performance Evaluation of a Novel Converged Architecture for Digital-Video Transmission Over Optical Wireless Channels

A robust channel coding architecture for multigigabit-per-second digital-video transmission over the optical wireless channel is introduced and evaluated. The proposed scheme combines low-density parity-check coding with channel interleaving to improve the transmission over turbulent temporally correlated optical wireless channels while satisfying real-time video delay constraints. Frame error rates of the presented code design are evaluated via simulation for intensity-modulation/direct-detection optical wireless links in both lognormal- and Rayleigh-fading channels. Results indicate that the performance of the proposed system is effective across a large range of atmospheric turbulence strengths and achieves significant temporal diversity in moderately long (10 ms) temporal correlation times while satisfying a 0.3-s real-time delay constraint. Moreover, the proposed design is shown to outperform the Reed-Solomon codes prevalent in the legacy fiber and wireless digital-television distribution systems.