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81.
To facilitate a study of the pharmacokinetics of drugs dissolved in sweat, a technique was devised for collecting sweat at a steady rate over an 8-day period. Three normal subjects each wore 4 absorbent pads applied to their skin under waterproof dressings for 8 days. The absorbent pads were either plain cotton or cotton impregnated with sodium chloride crystals. Each pad (3 cm x 3 cm) was applied to an area of skin (2 cm x 2 cm) defined by an adhesive template, and was removed daily, weighed, and replaced, to determine progressive uptake of sweat. Sweat uptake by plain cotton pads reached a plateau value within 2 to 3 days and did not significantly increase thereafter; in contrast, uptake by the salt-impregnated pads continued at a steady rate for the full 8 days of the study (mean rate 0.79 mg/cm2/hr, SD = 0.16, N = 6). This effect may be related to an osmotic gradient across the skin, but the physiologic mechanisms are not completely clear. This appears to be a convenient tool for the collection of sweat over long periods at a steady rate. 相似文献
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The effects of feeding diest containing either cholesterol (0.24% w/w) or cholestyramine (2.5% w/w) and of fasting on sterol synthesis in the liver, ileum, and lung of both male and female guinea pigs have been studied by measuring the incorporation by tissue slices of 14C-labeled acetate into total digitoninpredipitable sterols. Cholesterol feeding significantly decreased (P less than 0.05) sterol synthesis in the liver, ileum, and lung of the males and in the ileum of females. Cholestyramine feeding stimulated the rate of hepatic sterol synthesis 13-fold but did not significantly affect sterologenesis in the ileum. Sterol synthesis in the lung was significantly increased (P less than 0.05) but to a much lesser extent than in the liver. Fatty acid synthesis in the liver, ileum, and lung was not significantly affected by either cholesterol or cholestyramine feeding. In guinea pigs fasted for 24 hr, sterol synthesis was inhibited in all three tissues, the most pronounced effect occurring in the liver. Only in the lung was fatty acid synthesis significantly decreased (P less than 0.001) by fasting. Cholesterol feeding resulted in increased concentrations of cholesterol in the plasma and liver. Cholestyramine feeding reduced plasma cholesterol concentration by 81% in females and by 64% in males. However, it did not significantly change the tissue cholesterol concentrations. Fasting resulted in a significant increase (P less than 0.05) in plasma cholesterol concentration but did not effect the concentration of cholesterol in the tissues. It was concluded that in the normal guinea pig, the feedback inhibition produced by both cholesterol and also possibly by bile acids suppresses sterol synthesis in the liver to very low rates compared to those in the small intestine, where sterologenesis is not only less sensitive to the cholesterol negative feedback system than that in the liver, but also is not subject to regulation by the bile acid negative feedback system. 相似文献
84.
Colitis cystica profunda is a benign disease of the colon. Its importance lies in differentiating it from mucus-producing adenocarcinoma. It has rarely been described in the surgical literature. A review of records of patients seen at the Mayo Clinic produced 66 clinical cases of localized colitis cystica profunda, and in 21 patients the diagnosis was confirmed histologically. Follow-up, which was available in all patients, ranged from 2 months to 29 years, with a mean follow-up of more than 8 years. The data suggest that local excision is the preferred initial therapy. 相似文献
85.
Grant Douglas S.; Brewster Robert G.; Stierhoff Karen A. 《Canadian Metallurgical Quarterly》1983,9(1):63
Trained 14 Silver King pigeons in a symbolic matching-to-sample task in which food (access to grain) and no-food (blackout) samples were used. Responding to red test stimuli was reinforced on food sample trials, and responding to green test stimuli was reinforced on no-food sample trials. Samples of food and no food were rendered "expected" or "surprising" during subsequent testing by preceding samples with a previously established signal for either food (CS+) or no food (CS–). CS+ preceded food samples and CS– preceded no-food samples on surprising trials. Findings suggest that enhanced retention on surprising trials was apparent from the outset of testing to the extent that (a) surprising and expected trials were not markedly dissimilar to the trials of training, and (b) control over test responding by the CSs presented on expected and surprising trials was minimized. Results are discussed with regard to generalization decrement and directed remembering. It is concluded that surprising samples receive enhanced postperceptual processing and that the mechanism involved is that of retrieval-generated priming of short-term memory. (17 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
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Cloned genomic DNA sequences corresponding to various regions of the human type II procollagen gene were used to analyze the DNA from 78 normal volunteers. Southern hybridization experiments detected polymorphic HindIII, BamHI, and EcoRI sites. The presence of the polymorphic HindIII site results in a 7.0-kilobase (kb) band, and the absence of this site results in a 14.0-kb band. When present, the BamH1 polymorphic site yields a 4.8-kb band, and when absent, yields a 7.2-kb band. The presence of the EcoRI polymorphic site results in a 3.7-kb band, and its absence results in a 7.0-kb band. Each polymorphic site was mapped. Analyses of the data demonstrated that the sites are present in overall gene frequencies of .39 for HindIII, .04 for BamHI, and .02 for EcoRI. Gene frequencies of the polymorphic sites were also studied with respect to race. The polymorphic sites are present in a Hardy-Weinberg distribution in the study population. Study of an extended family demonstrated that the segregation of the HindIII polymorphic site is consistent with Mendelian inheritance. 相似文献
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89.
Wai?Yu Email author Stephen?Brewster Email author 《Universal Access in the Information Society》2003,2(2):105-124
This paper introduces the development of a multimodal data visualisation system and its evaluations. This system is designed to improve blind and visually impaired peoples access to graphs and tables. Force feedback, synthesized speech and non-speech audio are utilised to present graphical data to blind people. Through the combination of haptic and audio representations, users can explore virtual graphs rendered by a computer. Various types of graphs and tables have been implemented, and a three-stage evaluation has been conducted. The experimental results have proven the usability of the system and the benefits of the multimodal approach. The paper presents the details of the development and experimental findings, as well as the changes of role of haptics in the evaluation. 相似文献
90.