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991.
Historically, pyrolytic carbon has been a material for cardiovascular applications, but it has several properties suited for orthopedic uses as well. Pyrolytic carbon has an elastic modulus similar to bone and is highly fatigue resistant, but has not been used in orthopedics because of poor fixation to bone. Plasma sprayed hydroxylapatite (HA) has significantly improved the bonding of bone to titanium alloy implants. The effect of plasma-sprayed HA on pyrolytic carbon implants was investigated in this study. Cylindrical samples were implanted through a single cortex in Beagle femurs. The animals were sacrificed after 8 weeks, and a mechanical push-out test was performed on the implants immediately after explantation. Samples were microradiographed, stained for histology, and examined histomorphometrically. Interface strength for each type of implant was calculated. Pyrolytic carbon showed almost no attachment strength with an average strength value of 1.59 MPa. HA-coated pyrolytic carbon (8.71 MPa) yielded the same interfacial strength as HA-coated titanium (8.71 MPa). Histology revealed that bone was in direct apposition to all implants, both HA coated and noncoated. Failures occurred between the core material and the coating, or within the coating, but not at the bone/HA interface. Histomorphometry results confirmed that the two types of HA-coated implants had more bone apposition than the uncoated pyrolytic carbon implants. It was concluded that a plasma sprayed HA coating significantly improves the bone fixation of pyrolytic carbon. 相似文献
992.
We report here on five new cases of solid and cystic papillary neoplasm (SCPN) of the pancreas diagnosed by fine-needle aspiration cytology (FNAC). All cytologic samples were obtained by ultrasonography, and the smears were conventionally fixed and stained. Special histochemical and immunocytochemical stains were also performed in some samples. Cytology revealed in all but one case numerous pseudopapillary structures composed of fibrovascular stalks lined with one or more layers of bland-appearing, uniform tumor cells. The tumor cells had round-to-oval euchromatic nuclei with frequently folded smooth contours and one or two small nucleoli. Their cytoplasm often contained eosinophilic, PAS-positive, and diastase-resistant inclusions. Foamy cells, psammoma bodies, blood, and cellular debris were found in the background. The criteria for the differential diagnosis versus other pancreatic lesions are discussed in some detail, as is the role of immunocytochemistry (ICC). In the literature, only 28 cases of cytologically investigated SCPN have been reported to the best of our knowledge. The most helpful criteria for the conclusive identification of SCPN by FNAC include the pseudopapillary arrangement with bland-appearing tumor cells, and, especially, the finding of acidophilic, PAS-positive, and diastase-resistant cytoplasmic granules. 相似文献
993.
This paper discusses the findings of a quantitative study of nurses' willingness to care for patients with AIDS. It identifies several factors that enable nurses to confront the fear of AIDS nursing and to turn fear into compassion, and negative attitudes into positive ones. These factors include professional values, organizational support, group identity, patients' responses, spirituality, family and friends with AIDS, knowledge about AIDS, and the importance of personal choice. The value of these factors are further supported through personal statements made by nurses on AIDS-dedicated units as they express their feelings, thoughts, and positive experiences and expectations regarding AIDS nursing. 相似文献
994.
Residents and staff of a nursing home in a metropolitan area were randomly assigned to three conditions to: (a) test whether staff's use of operant behavioural management strategies (Condition 1) would cause a greater increase in residents' self-care behaviour than staff's use of mutual goal setting (Condition 2) or routine nursing care (Condition 3), and (b) examine how useful group scores were, compared to individual scores, in determining change in individual residents' behaviour due to treatment. In-service training was provided to staff in Conditions 1 and 2 but not in Condition 3. Over a period of 22 weeks, nursing staff encouraged subjects to perform targeted self-care tasks independently. Data analysis indicated significantly greater change in self-care behaviours for subjects in Condition 1 than for subjects in Conditions 2 and 3. However, visual inspection of data for each case revealed that individual scores were more useful than averages and differences between groups for determining the effectiveness of the clinical interventions. 相似文献
995.
OBJECTIVES: New methods are available to immortalize parenchymal cells from exocrine glands and kidney with retention of differentiation. Adaptation of this technology to small, single-donor biopsy material or surgical specimens could provide genetically homogeneous cells for functional analyses and correlation with genetic background and underlying biochemistry. To develop a methodology useful for renal sodium metabolism, epithelial cell line generation was tested in a hypertensive rat model with features similar to salt-sensitive hypertension in humans. This form of hypertension has a large genetic component and is prevalent in African Americans. DESIGN: Protocols were designed to immortalize primary cultures of microdissected proximal tubule epithelial cells from spontaneously hypertensive (SHR) and control, normotensive Wistar-Kyoto (WKY) rats. Immortalization was based on a replication-defective retrovirus coding for SV40 large T-antigen as positive cell cycle regulator. Transport competent cells that grow on porous filters to form confluent monolayers were selected. RESULTS: Several proximal tubule cell lines have been developed from SHR and WKY rats. The cells retain important differentiated features, such as epithelial polarity, low monolayer conductance, and sodium-succinate cotransport. They are suitable for analyses of electrolyte transport by electrophysiology or imaging of intracellular fluorescent indicator dyes, such as sodium-binding benzofuran isophthalate. CONCLUSION: Feasibility of generating epithelial cell lines from defined renal segments was demonstrated. The cells retain important transport function so that analyses of sodium metabolism and the influence of genetic background on it are possible. The methodology is applicable to human specimens. 相似文献
996.
LJ DeBowes D Mosier E Logan CE Harvey S Lowry DC Richardson 《Canadian Metallurgical Quarterly》1996,13(2):57-60
Male and female subjects (n = 125) were measured on a battery of anthropometric tests every six months for a period of five years. Using maturity status as the temporal component (pubescent assessment stages one through five), proportionality characteristics of subjects were monitored as they matured toward the adult form. Subjects were assigned to one of the Hi, Mid and Lo sub-groups based on their score at PA stage one for each of 15 proportionality characteristics. Subsequently, those in the Mid group were removed from the analysis, while male and female subjects were pooled for the final ANOVA model. The results showed that a number of variables remained stable from PA stages one through five. That is, differences between the Hi and Lo groups were maintained throughout maturation. Predominantly, these included the proportional body segment breadth measures. Proportionality characteristics of the upper limbs appeared to stabilise from mid-adolescence. However, those related to the lower limb were not stable. In other words, a significant difference for the lower limb variables at PA stage one was not maintained through stages four and five. The results raised some doubts as to the suitability of using lower limb proportions as selection criteria in talent identification programs. 相似文献
997.
998.
The limbic system seems to be involved in the pathophysiology of neuropsychiatric disorders, including dementia, schizophrenia, affective disorders, and amnestic disorders. These findings are subtle and largely went undetected until the advent of modern neuroimaging. This article discusses some of the neuroimaging findings in these disorders. 相似文献
999.
The cluster of differentiation (CD) antigen CD28 is a 44-kDa, disulphide-bonded, homodimeric glycoprotein, which is constitutively expressed on the surface of all murine T cells and the majority of human T cells. Ligation of CD28 by its counter receptor, B7, expressed on the surface of antigen presenting cells, has been shown to induce signals that, in synergy with those derived from engagement of the T cell receptor by an antigen bound to a major histocompatibility complex, enhance proliferation and cytokine production. Manipulation of this interaction can have dramatic effects on the outcome of T cell activation. Blocking CD28/B7 interactions may be useful in preventing unwanted activation in allergy and autoimmune diseases, whereas enhancing this interaction can promote tumour rejection. Thus, CD28 and its signalling pathways may prove to be useful targets in the development of new therapeutic treatments. 相似文献
1000.
Adequacy of amino acids in diets fed to lactating dairy cows 总被引:1,自引:0,他引:1
The Cornell Net Carbohydrate and Protein System was used to evaluate absorbable limiting amino acids (AA) for milk yield. The system was utilized to characterize whether diets in five previous experiments met AA requirements for milk protein synthesis. Twenty-nine treatment means for milk yield from 367 cows constituted the database for the evaluation. Using the mechanistic relationships of nutrient metabolism described in the Cornell system, absorbed amounts were predicted of each essential AA from a diet and milk yields allowed by the most limiting AA. Regression of observed milk yield (Y) on predicted milk yield (X) using all treatment means (n = 29) was Y = 2.3 + 0.799X (r2 = 0.72). The linear relationship was stronger using 6 treatment means (n = 18) when protein supplements were fed rather than when ruminally protected AA were fed or when AA were postruminally infused (Y = -1.8 + 0.983X; r2 = 0.93). The Cornell system predicted that, for diets based on corn, Met or Lys was limiting when soybean meal was the protein source, but Lys was limiting when corn gluten meal or brewers grains were the source of protein. By AA limitation, the Cornell system explained differences in milk yield for diets that differed in supplemental protein sources in some of the experiments. As determined from milk protein yields in these studies, requirements for individual essential AA were expressed as a percentage of dietary dry matter or total essential AA, and values were relatively constant among dietary treatments and experiments. 相似文献