Detection of West Nile virus by the polymerase chain reaction and analysis of nucleotide sequence variation

A polymerase chain reaction (PCR) assay was developed to rapidly detect and identify West Nile (WN) virus. The RNA from seven isolates of WN virus from six countries and four other flaviviruses (Kunjin, Japanese encephalitis, St. Louis encephalitis, and yellow fever viruses) was reverse-transcribed (RT) and amplified by PCR. The nucleotide sequences of the amplified products were determined by a rapid, automated DNA sequencing method. The WN virus RT/PCR assay detected the target gene segment of sequencing method. The WN virus RT/PCR assay detected the target gene segment of isolates from both the African-Middle Eastern group and the Indian group with a sensitivity of approximately 0.05 pg of viral RNA. Kunjin virus was the only other flavivirus tested that produced a band of the appropriate size. Five of seven WN virus isolates showed 92-98% homology in the nucleotide sequence of their PCR products. The sequence of one isolate was virtually identical to the published sequence of the Nigerian isolate (99.5% homology). No correlation was established between the degree of nucleotide homology, geographic location, time of isolation, or source of the isolates.     

Determination and Health Implication of the Erucic Acid Content of Broccoli Florets, Sprouts, and Seeds

The erucic acid content of broccoli florets, sprouts, and seeds was found to be about 0.8, 320, and 12100 mg/100 g, respectively. Using the erucic acid limit established for canola oil in the U.S.A. and Canada as a guideline, the estimated dietary intake of erucic acid from florets and sprouts was considered of little consequence, whereas in seeds a relatively small amount (about 35 g/wk) equaled our calculated exposure limit for erucic acid. Additionally, the most complete fatty acid distribution yet published for the various forms of broccoli are presented.     

Modeling and analysis of substrate coupling in integrated circuits

This paper describes a fast and accurate simulator for characterizing the effects of substrate coupling on integrated-circuit performance. The technique uses the electrostatic Green function of the substrate medium and the fast Fourier transform algorithm. It is demonstrated that this technique is suitable for optimization of layout for minimization of substrate coupling. Analysis of substrate coupling in different types of substrates and the utility of guard rings in different types of substrates is also discussed. Experimental verification of the models is presented     

Towards hybrid Triple Helix indicators: A study of university-related patents and a survey of academic inventors

This paper presents work directed at capturing the entrepreneurial and collaborative activity of university researchers. The Triple Helix points to the emergence of the entrepreneurial university as well as to an increasing overlay of activities in universities, industry and government. This study explores ways in which patent-based metrics could be utilized in a Triple Helix context, and how hybrid indicators could be developed by combining patent with survey data. More specifically, it aims to develop indicators that connect technological inventiveness of university researchers to both funding organizations and users, as well as to entrepreneurial activities by academics. The paper develops a simplified model of the innovation process to benchmark the relevance of the indicators to the Triple Helix. An analysis of Finnish academic patents illustrates that patent data can already provide useful indicators but, on its own, cannot provide information about how academic patents are interconnected with government or industry through funding or utilization links. An exclusive analysis of patents can point to patent concentrations on certain universities, to inventors and assignees, or to potential gaps in translating applied science into industrial technology. However, the patent data had to be combined with an inventor survey in order to relate academic patents more to their Triple Helix environment. The survey indicated that most patented academic inventions are connected to (often publicly funded) scientific research by the inventors and tend to be utilized in large firms rather than in start-up companies founded by academic entrepreneurs.     

Efficient model reduction in non-linear dynamics using the Karhunen-Loève expansion and dual-weighted-residual methods

 We look at the task of computing the time-evolution of a non-linear system for a long time, in our case under random external influences. Our specific example is the fatigue evaluation of a wind turbine. To facilitate such a computation, we look at a reduction of the computational effort by projecting everything on a low-dimensional basis. In this case we take the Karhunen-Loève basis generated from running the model a little while under the random loading. It is important that the error which is caused by this reduction process can be controlled. We estimate the error by dual or adjoint methods. This in turn allows the process of model reduction to be performed adaptively. Dedicated to the memory of Prof. Mike Crisfield, for his cheerfulness and cooperation as a colleague and friend over many years.     

The effects of goal difficulty on physiological arousal, cognition, and task performance.

Conducted 2 laboratory experiments with 117 undergraduates to examine (1) the effect of assigned goal difficulty on arousal (self-report and heart rate), cognition (perceived norm, self-efficacy strength, and personal goal), and behavioral (task performance) measures and (2) the role of heart rate as a mediator of the goal-difficulty–performance relation. All Ss performed a task requiring cognitive and physical responses. Results of both experiments demonstrate that assigned goal difficulty affected heart rate, cognition, and task performance and that heart rate change was positively related to the cognitive and behavioral measures. Regression analyses suggested that a cognitive–affective mechanism may mediate the goal-difficulty–performance relation. Discussion is focused on the theoretical and practical implications of integrating an arousal concept within goal-setting theory. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Characterization of the membrane-associating domain of the sperm adhesive protein, bindin

Male Swiss-Webster mice were rendered tolerant to morphine by subcutaneous implantation of a morphine pellet, each containing 75 mg morphine base, for 3 days. Mice implanted with placebo pellets served as controls. A high degree of tolerance to the analgesic effect of morphine developed as evidenced by decreased analgesic response to various doses of morphine. A selective kappa-opiate agonist, U-50,488H (8, 16 and 32 mg/kg, i.p.) produced dose-dependent analgesic and hypothermic effects in mice implanted with placebo pellets. A significant decrease in the analgesic and hypothermic effects of U-50,488H was observed in morphine tolerant mice as compared to placebo-treated mice. Mice were rendered tolerant to U-50,488H by injecting the drug (25 mg/kg, i.p.) twice daily for 4 days. Vehicle injected mice served as controls. Tolerance to the analgesic and hypothermic effects of U-50,488H in mice injected chronically with the drug was evidenced by the decreases in the intensity of these responses when compared to those observed in vehicle injected controls. Morphine produced a dose-dependent analgesic and hypothermic effects in mice injected chronically with vehicle but the intensity of these effects was significantly lower in mice injected chronically with U-50,488H. These results indicate that a substantial tolerance to analgesic and hypothermic effects of U-50,488H develops in morphine tolerant mice. The effect of chronic injections of U-50,488H on the binding of [3H]ethylketocyclazocine (EKC) and [3H]D-Ala2,MePhe4,Gly-ol5-enkephalin (DAMGO) to whole brain and spinal cord kappa- and mu-opiate receptors was determined.(ABSTRACT TRUNCATED AT 250 WORDS)