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This study examines the nature and extent of the relationship between stress levels and intentions to participate in a worksite smoking cessation program among male current smokers (n = 220) employed in an automobile manufacturing plant. A plantwide survey was conducted which measured job stress, nonjob stress, smoking behavior, and intent to participate. The results of polychotomous logistic regressions suggest that among the current smokers in this plant, job and nonjob stress were positively associated with workers' intentions to participate in a worksite smoking cessation program. Thus, contrary to the popular notion that stress diminishes the motivation to quit, employees under high levels of stress may be most receptive to educational interventions intended to persuade smokers to commit to quitting. 相似文献
104.
KL Hsu CH Tsai FT Chiang HM Lo CD Tseng SG Ho YZ Tseng 《Canadian Metallurgical Quarterly》1997,80(12):1635-1637
Percutaneous balloon pericardiotomy is effective and less invasive for the treatment of recurrent pericardial effusion. This study suggests that the double-balloon method with 1 longer and 1 shorter balloon is the procedure of choice for percutaneous balloon pericardiotomy. 相似文献
105.
The Escherichia coli Lac repressor (Lac system) and tetracycline responsive promoter (Tet system) systems have been used individually to regulate gene expression at the cellular as well as the organismal levels. In this study, these two systems were combined (designated Lac/Tet dual-inducible system) to regulate two inducible genes simultaneously in a single cell. The isopropyl-beta-D-thiogalactopyranoside (IPTG) and tetracycline (used for the operation of the Lac and the Tet systems) were non-cytotoxic to the cells when added together into the cells at around the optimal concentrations (IPTG: < or = 5 mM; tetracycline: < 1.5 micrograms). The rate and efficiency of induction and repression of two inducible genes regulated by the Lac/Tet dual-inducible system were similar to the results obtained when one inducible gene is regulated by one inducible system in a single cell. The Lac/Tet dual-inducible system could function in many cell lines, which was demonstrated by regulating the expression of beta-galactosidase and luciferase reporter genes in five tumor cell lines by transient transfection analysis. The feasibility of introducing a second inducible system into an already established inducible cell line was confirmed. Finally, we showed that the Lac/Tet dual-inducible system functions at translational and at functional levels in a stable cell line named 7-4-b, which contains the Ha-ras and bc1-2 inducible genes. In conclusion, this study extends the application of prokaryotic inducible systems from the regulation of a single gene to two genes and helps clarify the relationship between two genes and the effects of two genes on the cells. 相似文献
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107.
Dual-energy x-ray absorptiometry of raloxifene effects on the lumbar vertebrae and femora of ovariectomized rats 总被引:1,自引:0,他引:1
M Sato C McClintock J Kim CH Turner HU Bryant D Magee CW Slemenda 《Canadian Metallurgical Quarterly》1994,9(5):715-724
A new potential therapeutic agent for postmenopausal osteoporosis, raloxifene, previously known as keoxifene, was evaluated by x-ray densitometry and more traditional techniques in quantitating the short-term (4-5 weeks) effects of ovariectomy on bones from 6-month-old rats. A Hologic QDR 1000/W and, to a limited extent, a Lunar DPXL, was used to quantitate ovariectomy, estrogen replacement, and raloxifene effects on vertebrae, femora, and tibiae. Both instruments performed well with precisions of 1.6% (Hologic) and 0.9% (Lunar) for anesthetized rats, which improved to 0.4% (Hologic) and 0.5% (Lunar) when the same rats were frozen. The lumbar vertebrae L1-4 showed a 12% decrease in bone mineral density 4 weeks after ovariectomy, compared with a 9% decrease for femora. Tibiae were also examined, but edge-detection problems prevented reproducible analysis of this site in vivo. The decrease in bone mineral density postovariectomy, especially for femora, was found to include both an increase in the projected area and a slight but not significant decrease in the bone mineral content of L1-4 and femora. These changes in density parameters of femora were supported by a decrease in dry weight and volume and a marginal increase in the second moment of inertia I for the identical femora examined ex vivo. Examination of individual lumbar vertebrae L1-5 suggested that the bone mineral density of L3 changes most dramatically in response to ovariectomy, but present techniques lack the spatial resolution and precision to quantitate bone changes reliably in individual vertebrae. 17 beta-Estradiol administered at 100 micrograms/kg/day subcutaneously inhibited ovariectomy effects on L1-4 bone mineral density, femoral moment of inertia, dry weight, and volume and to a lesser extent, femoral bone mineral density. A nonsteroidal compound, raloxifene HCl, at 1 mg/kg/day per os, had bone effects and effects on body weight that were largely indistinguishable from those of 17 beta-estradiol; however, raloxifene did not produce the uterotrophic effects observed with estrogen. The half-maximal efficacious dose of raloxifene on L1-4 bone mineral density was between 0.1 and 1.0 mg/kg/day per os. These data show that dual-energy x-ray absorptiometry compares favorably with traditional methods in quantitating bone changes caused by ovariectomy in small rodents, that L1-4 is a more sensitive region than whole femora in evaluating the effect of estrogen deficiency on bone loss, and the raloxifene may have promise as a treatment for conditions characterized by excessive bone loss after ovariectomy. 相似文献
108.
CH Jensen 《Canadian Metallurgical Quarterly》1993,18(2):355-359
Intraosseous pressure was measured in the lunate of 10 patients undergoing surgery for Kienb?ck's disease. Using the styloid process of the radius and the capitate as reference points of bone pressure, I found a stasis rather than arterial insufficiency in the lunate. This pattern was observed regardless of the stage of the disease according to St?hl and regardless of the finding by technetium TC 99m-methylene diphosphonate scintigraphy. The significance of this finding is still unknown but questions the current concept that Kienb?ck's disease is caused by arterial insufficiency, because these results imply an interruption of the blood flow as a result of venous congestion. 相似文献
109.
Probabilistic prediction of high cycle fatigue reliability of high strength steel butt‐welded joints
R. BEN SGHAIER CH. BOURAOUI R. FATHALLAH G. DEGALLAIX 《Fatigue & Fracture of Engineering Materials & Structures》2010,33(9):575-594
The aim of this paper is to develop a probabilistic approach of high cycle fatigue (HCF) behaviour prediction of welded joints taking into account the surface modifications induced by welding and the post‐welding shot peening treatment. In this work, the HCF Crossland criterion has been used and adopted to the case of welded and shot peened welded parts, by taking into account the surface modifications which are classified as follows: (i) the compressive residual stresses, (ii) the surface work‐hardening, (iii) the geometrical irregularities and (iv) the superficial defects. The random effects due to the dispersions of: (i) the HCF Crossland criterion material characteristics (ii) the applied loading and (iii) the surface modifications parameters are introduced in the proposed model. The HCF reliability has been computed by using the ‘strength load’ method with Monte Carlo simulation. The reliability computation results lead to obtain interesting and useful iso‐probabilistic Crossland diagrams (PCD) for different welding and shot peening surface conditions. To validate the proposed method, the approach has been applied to a butt‐welded joint made of S550MC high strength steel (HSS). Four types of specimens are investigated: (i) base metal (BM), (ii) machined and grooved (MG) condition, (iii) As welded (AW) condition and (iv) as welded and shot peened (AWSP) condition. The comparison between the computed reliabilities and the experimental investigations reveals good agreement leading to validate the proposed approach. The effects of the different welded and post‐weld shot peened specimen's surface properties are analysed and discussed using the design of experiments (DoE) techniques. 相似文献
110.
KA Birkness M Deslauriers JH Bartlett EH White CH King FD Quinn 《Canadian Metallurgical Quarterly》1999,67(2):653-658
A tissue culture bilayer system that mimics some aspects of early alveolar infection by Mycobacterium tuberculosis was developed. This model incorporates human lung epithelial type II pneumocyte (A549) (upper chamber) and endothelial cell (lower chamber) layers separated by a microporous membrane. This construction makes it possible to observe and quantify the passage of bacteria through the two layers, to observe the interaction of the bacteria with the various cell types, and to examine the basic mechanisms of immune cell recruitment to the site of infection. After 10(7) organisms were added to the upper chamber we microscopically observed large numbers of bacteria attached to and within the pneumocytes and we determined by viable-cell counting that a small percentage of the inoculum (0.02 to 0.43%) passed through the bilayer into the lower chamber. When peripheral blood mononuclear cells were added to the lower chamber, microscopic examination indicated a migration of the mononuclear cells through the bilayer to the apical surface, where they were seen associated with the mycobacteria on the pneumocytes. The added complexity of the bilayer system offers an opportunity to define more precisely the roles of the various lung cell types in the pathogenesis of early tuberculosis. 相似文献