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21.
CH Chouard M Ouayoun B Meyer C Coudert T Séqueville G Bachelot J Génin 《Canadian Metallurgical Quarterly》1998,115(3):118-128
A new method to determine a rate constant and total reaction product using data of reaction product kinetics is proposed. The method is based on the solution of the system of irrational equations (for special case), which describes such processes. This method was used to determine the reaction rate constant of interaction of some monoclonal antibodies with the antigens immobilized on immunological plates. 相似文献
22.
The chronotropic effect of angiotensin II (Ang II) was studied in cultured neurons from rat hypothalamus and brain stem with the use of the patch-clamp technique. Ang II (100 nM) increased the neuronal spontaneous firing rate from 0.8 +/- 0.3 (SE) Hz in control to 1.3 +/- 0.4 Hz (n = 7, P < 0.05). The amplitude of threshold stimulation was decreased by Ang II (100 nM) from 82 +/- 4 pA to 62 +/- 5 pA (n = 4, P < 0.05). These actions of Ang II were reversed by the angiotensin type 1 (AT1) receptor antagonist losartan (1 microM). In the presence of tetrodotoxin, Ang II (100 nM) significantly increased the frequency and the amplitude of the Cd2+-sensitive subthreshold activity of the cultured neurons. Ang II also stimulated the subthreshold early afterdepolarizations (EADs) to become fully developed action potentials. Similar to the action of Ang II, the protein kinase C (PKC) activator phorbol 12-myristate 13-acetate (PMA, 100 nM) increased the firing rate from 0.76 +/- 0.3 Hz to 2.3 +/- 0.5 Hz (n = 6, P < 0.05) and increased the neuronal subthreshold activity. After neurons were intracellularly dialyzed with PKC inhibitory peptide (PKCIP, 5 microM), PMA alone, Ang II alone, or PMA plus Ang II no longer increased the action potential firing initiated from the resting membrane potential level. However, superfusion of PMA plus Ang II or Ang II alone increased the number of EADs that reached threshold and produced action potentials even in the presence of PKCIP (5 microM, n = 4). The actions of Ang II could also be mimicked by depolarizing pulse and K+ channel blockers (tetraethylammonium chloride or 4-aminopyridine). These results indicate that Ang II by activation of AT1 receptors increases neuronal excitability and firing frequency, and that this may involve both PKC dependent and -independent mechanisms. 相似文献
23.
In the present work we extend the model of dendrite formation to the case of submicron unipolar charged particles, where the main transport mechanism in convective Brownian diffusion and electrical effects due to the presence of charges on the particles and electric field around cylindrical collector. We present solutions for the case of slip flow around the fiber and free molecular flow around particles. 相似文献
24.
液态金属快速结晶的热力学驱动力 总被引:1,自引:0,他引:1
采用超高真空无容器悬浮熔炼技术使液态Fe和Ni的过冷度分别达200和235K,借助量热法测定出其比热各为48.6和40.5J·mol~(-1)·K~(-1).据此精确计算出深过冷Fe和Ni快速结晶的热力学驱动力△G_(LS),并与Turnbull模型和Dubey-Ramachandrarao模型的近似计算结果相比较,发现这两种近似模型在过冷度超过100K时均产生大于1%的偏差.进一步分析发现,△G_(LS)的计算偏差对深过冷液态金属中晶体形核率的影响极大,因此快速凝固研究中应尽可能精确计算△G_(LS). 相似文献
25.
白洋淀流域降水特性分析 总被引:3,自引:0,他引:3
选取白洋淀流域6个雨量站1959年-2009年日降水量资料,并以此为基础运用滑动平均法、Mann-Kendall非参数秩次检验法及小波分析法对该流域降水特性演变规律进行了分析。结果表明:在流域内,年降水量呈下降趋势且主要由年降水日数显著下降引起;年内各月降水量的不均匀程度呈减小趋势;不同量级降水频率波动较小,但呈现小强度降水比例增加、较大强度降水比例减少的结构变化;暴雨雨量和暴雨强度有较明显的下降趋势,降水潜力下降;年降水量存在4类尺度的周期变化规律,其中16a变化为主周期。 相似文献
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28.
D Ili? EA Almeida DD Schlaepfer P Dazin S Aizawa CH Damsky 《Canadian Metallurgical Quarterly》1998,143(2):547-560
In many malignant cells, both the anchorage requirement for survival and the function of the p53 tumor suppressor gene are subverted. These effects are consistent with the hypothesis that survival signals from extracellular matrix (ECM) suppress a p53-regulated cell death pathway. We report that survival signals from fibronectin are transduced by the focal adhesion kinase (FAK). If FAK or the correct ECM is absent, cells enter apoptosis through a p53-dependent pathway activated by protein kinase C lambda/iota and cytosolic phospholipase A2. This pathway is suppressible by dominant-negative p53 and Bcl2 but not CrmA. Upon inactivation of p53, cells survive even if they lack matrix signals or FAK. This is the first report that p53 monitors survival signals from ECM/FAK in anchorage- dependent cells. 相似文献
29.
Exercise has a noted effect on skin blood flow and temperature. We aimed to characterize the normal skin temperature response to exercise by thermographic imaging. A study was conducted on ten healthy and active subjects (age=25.8+/-0.7 years) who were exposed to graded exercise for determination of maximal oxygen consumption (VO2 max), and subsequently to constant loads corresponding to 50%, 70%, and 90% of VO2 max. The skin temperature response during 20 min of constant load exercise is characterized by an initial descending limb, an ascending limb and a quasi-steady-state period. For 50% VO2 max, the temperature decrease rate was - 0.0075+/-0.001 degrees C/s during a time interval of 390+/-47 s and the temperature increase rate was 0.0055+/-0.0031 degrees C/s during a time interval of 484+/-99 s. The level of load did not influence the temperature decrease and increase rates. In contrast, during graded load exercise, a continuous temperature decrease of -0.0049+/-0.0032 degrees C/s was observed throughout the test. In summary, the thermographic skin response to exercise is characterized by a specific pattern which reflects the dynamic balance between hemodynamic and thermoregulatory processes. 相似文献
30.