不同产地5种菊花氨基酸组成分析及营养价值评价

为防止品种混杂,筛选药用或茶用菊花的专用品种,以不同产地来源的8个药用菊花品种16个样品为试材,采用超高效液相色谱法和比色法测定不同产地药用菊花的品质成分含量;并基于主成分分析和聚类分析,对不同产地药用菊花品种产量与品质进行综合评价。结果表明,不同产地药用菊花品种单株花头数变异系数（39.03%）最大,是构成药用菊花单株花头干重的主要因素（r=0.800）,以枣庄红心菊单株花头数（496.50）最多、单株花头干重最高;绿原酸、3,5-O-二咖啡酰基奎宁酸和总黄酮等药效成分在不同来源的样本中差异显著（P<0.05）,上述三种成分在滁州产滁菊中相对含量较高,分别为3.75%、8.07%、16.08%,这与其品质成分综合评价结果一致,说明滁州滁菊药效较优。13个性状指标的主成分分析提取出药效因子、高产因子、总黄酮因子和花径因子4个主成分,其累计贡献率为84.34%;综合评价得分排在前3位的依次为枣庄引种的红心菊、早小洋菊、滁菊。结合聚类分析结果,判定在原产地,滁州滁菊适于药用;在枣庄引种地,红心菊为优选的高产品种,早小洋菊为综合品质优良的品种。通过建立药用菊花品种产量和品质的综合评价体系,可为我国药茶两用菊花新品种的引种筛选和药食同源利用提供理论依据。

     

不同污泥干化焚烧技术路线全链条碳足迹分析

碳足迹分析是目前国际上常用的技术能耗评估和资源优化配置的方法.文中采用IPCC《国家温室气体清单指南》提供的方法,对干化-焚烧、深度脱水-干化-焚烧、厌氧消化-深度脱水-干化-焚烧3种基于焚烧的污泥处理处置技术路线进行碳足迹分析.结果表明,污泥干化-焚烧技术路线净碳排放量最低.厌氧消化虽然是污泥资源化利用的重要方式,但...     

基于工作集的移动应用设计

在移动数据库环境中,同时保证数据的移动性、可用性和一致性是非常困难的事情。提出了工作集管理器机制,该机制在固定网络上维护用户工作集的不同版本,用户可以根据对数据的需求以及当前的网络情况,指定对哪些数据进行监控,同时指定在什么条件下生成新的视图版本。通过对标准SQL语句进行扩充,使得用户在数据移动性、可用性和一致性之间取得一种平衡。     

Microvascular decompression in glossopharyngeal neuralgia

Glossopharyngeal neuralgia is a rare and often controversial cause for odynophagia and otalgia. The otolaryngologist, head and neck surgeon may be the primary physician called upon to diagnose and treat this entity. In this study, vascular decompression, or more specifically, elimination of contact between the ninth cranial nerve and the posterior inferior cerebellar artery, was employed as treatment in three patients. All achieved relief of their symptoms with this intervention. A review of the neurosurgical literature and the experience with vascular decompression in trigeminal neuralgia, hemifacial spasm, and glossopharyngeal neuralgia is presented. The authors conclude that vascular decompression is effective in carefully selected patients, and the role of the skull base surgeon in managing this problem is expanding.     

Prevalence, genetics and clinical presentation of chronic granulomatous disease in Sweden

To estimate the prevalence of chronic granulomatous disease (CGD) in Sweden, an inquiry asking for known and possible CGD cases was mailed to paediatric, internal medicine and infectious disease departments all over Sweden. The detected patients were characterized as to genetics and the clinical presentation. Twenty-one patients (belonging to 16 different families) were found, corresponding to a prevalence of approximately 1/450,000 individuals. The patients with X-linked disease, lacking a functional gp91phox protein (n = 12), comprised 57% and 43% of the patients had an autosomal recessive (AR) disease lacking p47phox (n = 7) or p67phox (n = 1), respectively. All unrelated patients with X-linked disease displayed different gene abnormalities such as point mutations predicting nonsense (n = 3), missense (n = 1) or splice site mutations (n = 2), but also a total deletion and a unique 40 base pair duplicature insertion. The patients with p47phox-deficiency showed a GT deletion at a GTGT tandem repeat, and the p67phox-deficient patient displayed a heterozygous in-frame deletion of AAG combined with a large deletion in the other allele. Three patients died during the study period, two from pseudomonas cepacia infections. Patients with X-linked disease had more frequent infections (mean of 1.7 per year), than the patients with AR inheritance (0.5 infections per year). The most common infections were dermal abscesses (n = 111), followed by lymphadenitis (n = 82) and pneumonias (n = 73). Inflammatory bowel disease-like symptoms, mimicking Crohn's disease of the colon, was seen in three CGD patients.     

The murine CYLN2 gene: genomic organization, chromosome localization, and comparison to the human gene that is located within the 7q11.23 Williams syndrome critical region

Several agents that act through G-protein-coupled receptors and also stimulate phosphoinositide-specific phospholipase C (PI-PLC), including angiotensin II, vasopressin, norepinephrine, and prostaglandin (PG) F2alpha, activated the ERK1 (p44mapk) and ERK2 (p42mapk) members of the mitogen-activated protein (MAP) kinase family in primary cultures of rat hepatocytes, measured as phosphorylation of myelin basic protein (MBP) by a partially purified enzyme, immunoblotting, and in-gel assays. All these agonists induced a peak activation (two to threefold increase in MBP-phosphorylation) at 3-5 min, followed by a brief decrease, and then a sustained elevation or a second increase of the MAP kinase activity that lasted for several hours. Although all the above agents also stimulated PI-PLC, implicating a Gq-dependent pathway, the elevations of the concentration of inositol (1,4,5)-trisphosphate did not correlate well with the MAP kinase activity. Furthermore, pretreatment of the cells with pertussis toxin markedly reduced the MAP kinase activation by angiotensin II, vasopressin, norepinephrine, or PGF2alpha. In addition, hepatocytes pretreated with pertussis toxin showed a diminished MAP kinase response to epidermal growth factor (EGF). The results indicate that agonists acting via G-protein-coupled receptors have the ability to induce sustained activation of MAP kinase in hepatocytes, and suggest that Gi-dependent mechanisms are required for full activation of the MAP kinase signal transduction pathway by G-protein-coupled receptors as well as the EGF receptor.     

Rapid progression to end-stage renal disease in young hypertensive African Americans with proteinuria

The effect of progesterone (P) on pancreatic islet-cell proliferation and function of cyclic and pregnant rats was investigated in vivo. Silastic tubes containing P were inserted s.c. in cyclic rats for 7 or 14 days and in pregnant rats from day 7 to 14, from day 14 to 21 or from day 7 to 21 of pregnancy. 5-Bromo-2-deoxyuridine (BrdU) was infused during the last 24h of the treatment; the proportion of dividing islet-cells was determined in pancreatic sections, which were immunostained for BrdU. Islet-cell function was determined by measuring glucose and insulin response to a standard intravenous glucose challenge. P treatment increased P and 20alpha-dihydroprogesterone (20alpha-OHP) levels in cyclic rats; in pregnant rats, only the plasma levels of 20alpha-OHP were elevated. Both 7 and 14 days of P treatment stimulated islet-cell proliferation in cyclic rats. In pregnant rats, P treatment increased islet-cell proliferation on day 14, but not on day 21 after either 7 or 14 days of P treatment. P did not affect plasma lactogenic activity in pregnant rats; plasma concentrations of prolactin were decreased after 14 days of P treatment in cyclic rats, but were not affected in pregnant rats. P treatment had no effect on glucose tolerance and glucose-stimulated insulin secretion in any of the groups. It was concluded that: 1. in vivo P stimulates islet-cell proliferation, but does not affect islet-cell function, 2. the stimulatory effects of P are indirect and possibly mediated by the P metabolite 20alpha-OHP and 3. at the end of gestation, stimulation of islet-cell proliferation is inhibited by some factor, which is not identical to P.     

集成化智能模具CAD系统研究

介绍了集成化智能模具CAD系统的体系结构，包括基于特征的模具CAD集成信息模型，和基于黑板推理的智能决策模型，并以实例说明该模具CAD系统的集成和智能推理工作过程。