Detection of muramidase (lysozyme)-secreting leucocytes at the single-cell level by a protein A plaque assay

Using a modification of the protein A plaque assay, muramidase (lysozyme)-producing leucocytes were detected as plaque-forming cells. In the presence of anti-muramidase Ig and complement the secreted lysozyme resulted in lysis of protein-A-coated target erythrocytes. By the use of a monolayer technique individual plaque-forming cells could be identified by staining procedures. Granulocytes as well as monocytes were found to produce muramidase and thus to form plaques. This method could serve as a useful tool when studying lysozyme secretion. Furthermore, by the use of appropriate antisera, this method could be employed for the study of any cell type (any secretion), provided enough molecules are being secreted.     

基于DRM技术的IPTV版权保护模型研究

随着网络的迅猛发展,IPTV通过网际协议(IP)来提供包括数字电视节目在内的多种数字多媒体服务及增值业务。由于IPTV的核心是内容传播,所以内容的版权管理是IPTV的一个关键问题。本文介绍了数字版权管理的基本原理,分析了现有DRM系统的缺点,提出了一种IPTV系统的数字版权管理改进模型,详细阐述了其工作流程,并对其相关性能进行分析。     

Unsupervided pattern recognition for the classification of EMG signals

The shapes and firing rates of motor unit action potentials (MUAP's) in an electromyographic (EMG) signal provide an important source of information for the diagnosis of neuromuscular disorders. In order to extract this information from EMG signals recorded at low to moderate force levels, it is required: i) to identify the MUAP's composing the EMG signal, ii) to classify MUAP's with similar shape, and iii) to decompose the superimposed MUAP waveforms into their constituent MUAP's. For the classification of MUAP's two different pattern recognition techniques are presented: i) an artificial neural network (ANN) technique based on unsupervised learning, using a modified version of the self-organizing feature maps (SOFM) algorithm and learning vector quantization (LVQ) and ii) a statistical pattern recognition technique based on the Euclidean distance. A total of 1213 MUAP's obtained from 12 normal subjects, 13 subjects suffering from myopathy, and 15 subjects suffering from motor neuron disease were analyzed. The success rate for the ANN technique was 97.6% and for the statistical technique 95.3%. For the decomposition of the superimposed waveforms, a technique using crosscorrelation for MUAP's alignment, and a combination of Euclidean distance and area measures in order to classify the decomposed waveforms is presented. The success rate for the decomposition procedure was 90%.     

逆向工程实体模型重建技术研究

针对CMM扫描线数据,进行了数据分层和数据排序,完成了数据拓扑关系的建立.研究了距离阈值法和向量角度法相结合的数据精简方法和基于分层维的特征提取技术.以基于分层维的数据处理技术研究为基础,提出了一种利用数据点特征的非均匀有理B样条(NURBS)曲面重构方法,在Solidworks中实现了实体模型的重建.     

基于高性能单片机的双CPU通用数控系统

讨论了基于高性能单片机的双CPU的通用数控系统的设计。系统采用两个高性能单片机为处理器，处理器间相互通信，协调工作。软件采用模块化设计。使用时针对具体数控系统功能要求，将通用系统略加增删，便能满足客户需求，体现了数控系统的通用性。通过在STAR-06TQ数控涂漆机上的实际应用，证明本系统能够满足预期设计要求。     

一种改善BLDCM调速性能的模糊矢量控制策略

针对永磁无刷直流电机(BLDCM)在方波控制时转矩脉动大、电流畸变程度大以及反电势不稳定造成转速波动的问题,本文在分析BLDCM控制方式的基础上,将这些问题的原因归结于控制时的电流换相以及反电势并非理想方波电势。基于此,针对电动汽车BLDCM提出一种基于模糊控制的无刷直流电机矢量控制调速策略,此策略使用模糊控制器对转速误差进行调节,进而增强系统的调速性能;使用矢量控制取代方波控制,进而克服转矩脉动、电流畸变以及反电势不稳定等问题。实验结果表明,本文提出的控制策略能较好地抑制转矩脉动,并使电流以及转速更加平滑稳定。     

Effect of sucrose acetate isobutyrate (SAIB) ingestion on the hepatobiliary function of normal human male and female volunteers

Monitoring of oxygen uptake during general anesthesia would have several benefits, but unfortunately, this is usually not available in the clinical routine situation. The herein proposed formula to calculate oxygen uptake (.VO2) necessitates only the accurate measurement of FIO2 as well as fresh gas flow and composition. Additionally, this method is not affected by the presence of anesthetic gases. The calculation uses the difference in oxygen content between the delivered fresh gas and the resulting FIO2 in the anesthesia circle system. This gap originates from oxygen uptake (that is mainly caused by metabolic oxygen consumption) and is more pronounced if low fresh gas flows are administered. In order to obtain representative results, calculation of .VO2 should be performed only after achievement of respiratory steady state conditions. Due to its simplicity and wide availability, it has the potential to become a valuable extension in anesthesia monitoring during the performance of routine general anesthesia.     

Flt3high and Flt3low CD34+ progenitor cells isolated from human bone marrow are functionally distinct

We generated monoclonal antibodies against the human Flt3 receptor and used them to study the characteristics of normal human bone marrow cells resolved based on Flt3 expression. Human CD34+ or CD34+lin- marrow cells were sorted into two populations: cells expressing high levels of Flt3 receptor (Flt3high) and cells with little or no expression of Flt3 receptor (Flt3low). Flt3 receptor was detected on a subset of CD34+CD38- marrow cells, as well as on CD34+CD19+ B lymphoid progenitors and CD34+CD14+CD64+ monocytic precursors. Flt3 receptor was also present on more mature CD34-CD14+ monocytes. In colony-forming assays, Flt3high cells gave rise mainly to colony-forming unit-granulocyte-macrophage (CFU-GM) colonies, whereas Flt3low cells produced mostly burst-forming unit-erythroid colonies. There was no difference in the number of multilineage CFU-Mix colonies between the two cell fractions. Cell cycle analysis showed that a large number of the Flt3low cells were in the G0 phase of the cell cycle, whereas Flt3high cells were predominantly in G1. Cell numbers in the suspension cultures initiated with Flt3high cells were maintained in the presence of Flt3 ligand (FL) alone, and increased in response to FL plus kit ligand (KL). In contrast, cell numbers in the suspension cultures started with Flt3low cells did not increase in the presence of FL, or FL plus KL. Upregulation of Flt3 receptor on Flt3low cells was not detected during suspension culture. CD14+ monocytes were the major cell type generated from CD34+lin-Flt3high cells in liquid suspension culture, whereas cells generated from CD34+lin-Flt3low cells were mainly CD71+GlycA+ erythroid cells. These results show clear functional differences between CD34+Flt3high and CD34+Flt3low cells and may have implications concerning the in vitro expansion of human hematopoietic progenitor cells.