Thermal process validation for Escherichia coli O157:H7, Salmonella, and Listeria monocytogenes in ground turkey and beef products

At 55 to 70 degrees C, thermal inactivation D-values for Escherichia coli O157:H7, Salmonella, and Listeria monocytogenes were 19.05 to 0.038, 43.10 to 0.096, and 33.11 to 0.12 min, respectively, in ground turkey and 21.55 to 0.055, 37.04 to 0.066, and 36.90 to 0.063 min, respectively, in ground beef. The z-values were 5.73, 5.54, and 6.13 degrees C, respectively, in ground turkey and 5.43, 5.74, and 6.01 degrees C, respectively, in ground beef. In both ground turkey and beef, significant (P < 0.05) differences were found in the D-values between E. coli O157:H7 and Salmonella or between E. coli O157:H7 and L. monocytogenes. At 65 to 70 degrees C, D-values for E. coli O157:H7, Salmonella, and L. monocytogenes were also significantly (P < 0.05) different between turkey and beef. The obtained D- and z-values were used in predicting process lethality of the pathogens in ground turkey and beef patties cooked in an air impingement oven and confirmed by inoculation studies for a 7-log (CFU/g) reduction of E. coli O157:H7, Salmonella, and L. monocytogenes.     

Potential phytotoxic components of pulverised fuel ash

The release of aluminium, arsenic, boron, cobalt, copper, chromium, manganese, nickel, lead and zinc from pulverised fuel ash (PFA) and a standard Wellesbourne soil were studied by extraction with nitric acid and EDTA and by anaerobic incubation with grass meal. According to each of the criteria, only aluminium, arsenic, boron and copper were released in appreciably greater quantities from PFA than from soil. There were considerable variations in pH and the total content of soluble salts, mainly sodium, potassium, magnesium and calcium sulphates, between samples of ash. pH varied from 4.6 to 9.3 and the conductivity declined from 4.75 × 10^?3 to 3.2 × 10^?3 ohm^?1 cm^?1 with increased pH. The pH of the ash also influenced the concentration of other ions in water in equilibrium with it. When the pH was low the concentrations of aluminium and copper were large but those of arsenic were small. By contrast, when pH was high, the concentrations of arsenic were substantial and those of aluminium and copper small. The concentrations of boron, though always substantial, declined with increase in pH. It is concluded that, in addition to sulphate and boron, aluminium, copper and arsenic are potential causes of phytotoxicity.     

Stable dye-labelled oligonucleotide-nanoparticle conjugates for nucleic acid detection

Metallic nanoparticles functionalized with oligonucleotides are used for a number of nucleic acid detection strategies. However, oligonucleotide-nanoparticle conjugates suffer from a lack of stability when exposed to certain conditions associated with DNA detection assays. In this study, we report the synthesis of thiol and thioctic acid-modified oligonucleotide gold nanoparticle (OGNs) conjugates functionalized with a dye label and varying spacer groups. The thioctic acid-modified conjugates exhibit increased stability when treated with dithiothreitol (DTT) compared to the more commonly used thiol modification. When the dye labelled oligonucleotide nanoparticle conjugates are exposed to the same conditions there is a pronounced increase in the stability for both thioctic acid and thiol modified sequences. These results open up the possibility of simply using a dye label to enhance the stability of oligonucleotide-nanoparticle conjugates in DNA detection assays where the enhanced stability of the conjugate system can be advantageous in more complex biological environments.     

Techno-economic comparisons of hydrogen and synthetic fuel production using forest residue feedstock

Mobile distributed pyrolysis facilities have been proposed for delivery of a forest residue resource to bio-fuel facilities. This study examines the costs of producing hydrogen or synthetic petrol (gasoline) and diesel from feedstock produced by mobile facilities (bio-oil, bio-slurry, torrefied wood). Results show that using these feedstock can provide fuels at costs competitive to conventional bio-fuel production methods using gasification of a woodchip feedstock. Using a bio-oil feedstock in combination with bio-oil steam reforming or bio-oil upgrading can produce hydrogen or petrol and diesel at costs of 3.25 $ kg⁻¹ or 0.86 $ litre⁻¹, respectively, for optimally sized bio-fuel facilities. When compared on an energy basis ($ GJ⁻¹), hydrogen production costs tend to be lower than those for synthetic petrol or diesel production across a variety of bio-fuel production pathways.     

Co-encapsulation of fish oil with phytosterol esters and limonene by milk proteins

Co-encapsulated microcapsules containing three lipophilic bioactive components (LBCs) including fish oil, phytosterols and limonene were studied and compared with those containing only fish oil. Milk proteins (whey protein isolate and sodium caseinate in a ratio of 4:1) were used as wall materials. Results show that good quality microcapsules can be obtained at inlet temperature of 170 °C and outlet temperature of 70 °C, with the wall to core ratio of 4:1. There was no significant different (p > 0.05) in the microencapsulation efficiency and the oxidation indicators (PV and AV) after the accelerated storage for both types of microcapsules. However, the retention of EPA and DHA in the LBCs-microcapsules was significantly higher (p < 0.05) than the fish oil microcapsules. The phytosterols content was unchanged but loss of limonene occurred after storage. The LBCs-microcapsules had better flavor/odour profile than the fish oil microcapsules after drying and storage.     

Using a Fragment‐Based Approach To Target Protein–Protein Interactions

The ability to identify inhibitors of protein–protein interactions represents a major challenge in modern drug discovery and in the development of tools for chemical biology. In recent years, fragment‐based approaches have emerged as a new methodology in drug discovery; however, few examples of small molecules that are active against chemotherapeutic targets have been published. Herein, we describe the fragment‐based approach of targeting the interaction between the tumour suppressor BRCA2 and the recombination enzyme RAD51; it makes use of a screening pipeline of biophysical techniques that we expect to be more generally applicable to similar targets. Disruption of this interaction in vivo is hypothesised to give rise to cellular hypersensitivity to radiation and genotoxic drugs. We have used protein engineering to create a monomeric form of RAD51 by humanising a thermostable archaeal orthologue, RadA, and used this protein for fragment screening. The initial fragment hits were thoroughly validated biophysically by isothermal titration calorimetry (ITC) and NMR techniques and observed by X‐ray crystallography to bind in a shallow surface pocket that is occupied in the native complex by the side chain of a phenylalanine from the conserved FxxA interaction motif found in BRCA2. This represents the first report of fragments or any small molecule binding at this protein–protein interaction site.