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101.
P M Reid A E Wilkinson K C Leung M N Jones 《Computer applications in the biosciences》1989,5(2):133-135
Analytical ultracentrifugation is commonly used for the determination of molecular weights (sedimentation equilibrium) and sedimentation coefficients (sedimentation rate) of biological macromolecules in solution. A Turbo Pascal program for the analysis of sedimentation equilibrium centrifugation data produced by absorbance optical systems is described. The user may enter data from a scan of absorbance versus distance from the centre of rotation, via a graphics tablet (or ASCII file). This is subsequently manipulated to yield an apparent weight average molecular weight for the given sample. Plots of ln (absorbance) versus (radius2) may also be produced. The method described uses readily available computational equipment requiring only a graphics tablet in addition to an IBM PC compatible computer. This technique and the software developed have been used to investigate the molecular weight range of two International Humic Substances Society (IHSS) reference samples from the Suwannee River. 相似文献
102.
An outline of the characteristics of PEEK and the versatility of its compositional forms (micro and macro composites) are given to illustrate its wide potential for success in engineering applications. Although it is necessary to have particular tabulations of mechanical properties for engineering design, these are seldom available and consequently it is argued that an understanding of stiffness, toughness and strength properties are required to fully exploit available manufacturer's data and thus develop the full potential of PEEK and its composites. Stiffness characteristics are considered in terms of a modulus function which is dependent on time under load and temperature. In its composite forms, whether reinforced with short or continuous fibres, stiffness anistropy can be both considerable and complex, but some empirical ground-rules are apparent. For continuous fibre composites even in the form of complex lay-ups, it is also possible to attempt some stiffness prediction from certain pseudo-elastic constants. Toughness of PEEK and its composites is described in terms of both comparative and intrinsic properties. Instrumented falling weight impact data, particularly as a function of temperature enable some insight into ductile-brittle transitions for the unreinforced material, but crack initiation and crack propagation processes for the various fibre reinforced forms. Intrinsic toughness is described in terms of linear elastic fracture mechanics theory. Strength properties are described for static and dynamic loading configurations. In particular, PEEK and its composites are evaluated for increasing test severities for strength characteristics; stress concentration, loading form and test temperature are considered. 相似文献
103.
The noise generated by a ball mill during a batch grinding operation is investigated and the results show that, for a given ore, the noise levels may vary with time of grind, ore charge weight and mill speed. The role of the ore in absorbing noise energy is suggested as a possible control variable for the grinding process and as an indicator of product size distribution. The relationship between ore types and mill noise is also examined and, under conditions of wet grinding, it is demonstrated that mill noise analysis can indicate ore type and may have a possible use as a grindability-type parameter. 相似文献
104.
A computationally efficient discrete Backus-Gilbert (BG) method is derived that is appropriate for resolution-matching applications using oversampled data. The method builds upon existing BG methods and approximation techniques to create a modified set of BG coefficients. The method in its current form is restricted to a resolution-only minimization constraint, but in the future could be extended to use a simultaneous noise minimization constraint using a generalized singular value decomposition (GSVD) approach. A theoretical one-dimensional intercomparison is performed using a hypothetical sensor configuration. A comparison of the discrete BG method with a nondiscrete BG method shows that the new approach can be 250% more efficient while maintaining similar accuracies. In addition, an SVD approximation increases the computational efficiencies an additional 43%-106%, depending upon the scene. Several quadrature methods were also tested. The results suggest that accuracy improvements are possible using customized quadrature in regions containing known physical data discontinuities (such as along coastlines in microwave imagery data). The ability to recompute the modified BG coefficients dynamically at lower computational cost makes this work applicable toward applications in which noise may vary, or where data observations are not available consistently (e.g. in radio frequency interference contaminated environments). 相似文献
105.
Beynon M.J. Jones L. Holt C.A. 《IEEE transactions on systems, man, and cybernetics. Part A, Systems and humans : a publication of the IEEE Systems, Man, and Cybernetics Society》2006,36(1):173-186
In this paper, a novel object classification method is introduced and developed within a biomechanical study of human knee function in which subjects are classified to one of two groups: subjects with osteoarthritic (OA) and normal (NL) knee function. Knee-function characteristics are collected using a three-dimensional motion-analysis technique. The classification method transforms these characteristics into sets of three belief values: a level of belief that a subject has OA knee function, a level of belief that a subject has NL knee function, and an associated level of uncertainty. The evidence from each characteristic is then combined into a final set of belief values, which is used to classify subjects. The final belief values are subsequently represented on a simplex plot, which enables the classification of a subject to be represented visually. The control parameters, which are intrinsic to the classification method, can be chosen by an expert or by an optimization approach. Using a leave-one-out cross-validation approach, the classification accuracy of the proposed method is shown to compare favorably with that of a well-established classifier-linear discriminant analysis. Overall, this study introduces a visual tool that can be used to support orthopaedic surgeons when making clinical decisions. 相似文献
106.
Burkholderia pseudomallei is the causative agent of melioidosis, a disease increasingly recognized as an important cause of morbidity and mortality in many regions of the world. B. pseudomallei is a facultative intracellular pathogen capable of invading eukaryotic cells. We used Tn5-OT182 mutagenesis to generate mutants deficient in the ability to invade a human type II pneumocyte cell line (A549 cells). One of these mutants, AJ1D8, exhibited approximately 10% of the ability of the parental strain, 1026b, to invade A549 cells. There was no difference in the abilities of 1026b and AJ1D8 to resist killing by RAW macrophages or the human defensin HNP-1. The nucleotide sequence flanking the Tn5-OT182 integration in AJ1D8 was determined, and two open reading frames were identified. The predicted proteins shared considerable homology with two-component regulatory systems involved in the regulation of heavy-metal resistance in other organisms. AJ1D8 was 16-fold more sensitive to Cd2+ and twofold more sensitive to Zn2+ than was 1026b but was not sensitive to any of the other heavy metals examined. The B. pseudomallei two-component regulatory system, termed irlRS, complemented the invasion-deficient and heavy-metal-sensitive phenotype of AJ1D8 in trans. There was no significant difference between the virulence of AJ1D8 and that of 1026b in infant diabetic rats and Syrian hamsters, suggesting that the irlRS locus is probably not a virulence determinant in these animal models of acute B. pseudomallei infection. 相似文献
107.
Pre-clerking of all patients undergoing elective general surgical operations was introduced at our hospital in an attempt to reduce an unacceptably high operation cancellation rate. A prospective audit has been performed on the effect of this policy on the cancellation rate. Before the introduction of pre-clerking there was a marked seasonal variation in the number of patients who failed to attend for surgery, which could be explained by absence on holiday. This seasonal variation disappeared after the start of pre-clerking clinics, but there has been no reduction in the number of cancellations for medical reasons. 相似文献
108.
Hyperdynamic circulation of cirrhotic rats: role of substance P and its relationship to nitric oxide
CJ Chu FY Lee SS Wang FY Chang YT Tsai HC Lin MC Hou SL Wu CC Tai SD Lee 《Canadian Metallurgical Quarterly》1997,32(8):841-846
BACKGROUND: It has been suggested that excessive formation of nitric oxide (NO) is responsible for the hyperdynamic circulation observed in portal hypertension. Substance P is a neuropeptide partly cleared by the liver and causes vasodilatation through the activation of the endothelial NO pathway. However, there are no previously published data concerning the plasma level of substance P in cirrhotic rats and its relationship to NO. METHODS: Plasma concentrations of substance P and nitrate/nitrite (an index of NO production) were determined in control rats and cirrhotic rats with or without ascites using an enzyme-linked immununosorbent assay and a colorimetric assay, respectively. In addition, systemic and portal hemodynamics were evaluated by a thermodilution technique and catheterization. RESULTS: Cirrhotic rats with and without ascites had a lower systemic vascular resistance (2.6 +/- 0.2 and 3.9 +/- 0.4 mmHg ml(-1) x min x 100 g body weight, respectively) and higher portal pressure (14.6 +/- 0.6 and 11.3 +/- 1.8 mmHg) than control rats (6.5 +/- 0.3 mmHg x ml(-1) x min x 100 g BW and 6.8 +/- 0.2 mmHg, respectively, P < 0.05), and cirrhotic rats with ascites had the lowest systemic vascular resistance. Plasma levels of nitrate/nitrite progressively increased in relation to the severity of liver dysfunction (control rats, 2.7 +/- 0.5 nmol/ml; cirrhotic rats without ascites, 5.6 +/- 1.3 nmol/ml; cirrhotic rats with ascites, 8.3 +/- 2.2 nmol/ml; P < 0.05). Cirrhotic rats with ascites displayed higher plasma values of substance P (57.7 +/- 5.9 pg/ml) than cirrhotic rats without ascites (37.9 +/- 3.1 pg/ml, P < 0.05) and control rats (30.1 +/- 1.0 pg/ml, P < 0.05). There was no significant difference in plasma substance P values between control rats and cirrhotic rats without ascites (P > 0.05). No correlation was found between plasma levels of substance P and nitrate/nitrite (r = 0.318, P > 0.05). CONCLUSIONS: Excessive formation of NO may be responsible, at least partly, for the hemodynamic derangements in cirrhosis. Although substance P may not participate in the initiation of a hyperdynamic circulation in cirrhosis, it may contribute to the maintenance of the hyperdynamic circulation observed in cirrhotic rats with ascites. 相似文献
109.
110.
Investigation of driving performance, vection, postural sway, and simulator sickness in a fixed-based driving simulator 总被引:1,自引:0,他引:1
This paper reports the results of a pilot study conducted to determine whether there is a relationship between simulator sickness and measures of driver inputs, vection, and postural sway, in a fixed base driving simulator. Seven males and four females performed a series of alternating left and right turns (20 each), with straigth-aways inbetween. The task was designed to promote experiences of circular and linear vection, and associated sway. It was hypothesized that greater driver inputs (i.e., steering wheel and braking activity; deviation from the centerline) would correlate: with reports of vection and measures of postural sway. Ten of the eleven subjects reported moderate sickness, which precluded any statistical comparisons between “sick” and “not sick” however, there was a significant tendency for persons who reported vection to also report sickness. There were no gender differences in reported incidence of vection or sickness, however, males tended to exhibit greater lateral velocity. The next phase of investigation will necessitate reducing the strength of the stimulus (i.e., require less turns) to increase variability of sickness scores amongst subjects and permit comparisons between “sick” and “not sick.” Measurement considerations include increasing the sensitivity of vection ratings, and examination of the timecourse for the development of postural sway. 相似文献