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71.
CK Miyasaka JA de Souza RP Torres JM Filho FM Lajolo R Curi 《Canadian Metallurgical Quarterly》1998,30(5):759-762
Expression of retinoblastoma (Rb) protein was immunohistochemically examined in laryngeal squamous cell neoplasias from 72 patients. Staining patterns were considered with reference to such prognostic factors as patient's age, histologic grade, tumour size and lymph node status, and 5-year survival rate. Rb protein negativity, either partial or complete, was noted in 28.8% of cases and was associated with a significantly lower 5-year survival rate, as well as with a higher likelihood of lymph node metastasis. This suggests that Rb alteration may be a prognostic indicator in patients with laryngeal carcinoma. 相似文献
72.
WR Schwan SN Coulter EY Ng MH Langhorne HD Ritchie LL Brody S Westbrock-Wadman AS Bayer KR Folger CK Stover 《Canadian Metallurgical Quarterly》1998,66(2):567-572
Staphylococcus aureus is an important pathogen of humans and other animals, causing bacteremia, abscesses, endocarditis, and other infectious syndromes. A signature-tagged mutagenesis (STM) system was adapted for use in studying the genes required for in vivo survival of S. aureus. An STM library was ultimately created in S. aureus RN6390, with Tn917 being used to create the transposon mutations. Pools of S. aureus RN6390 mutants were screened in mouse abscess, bacteremia, and wound infection models for growth attenuation after in vivo passage. One of the mutants that was identified displayed marked attenuation following large-pool screening in all three animal models, which was confirmed in bacteremia and endocarditis models of infection with a smaller pool of mutants. Sequence analysis of the entire open reading frame showed a 99% identity to the high-affinity proline permease (putP) gene characterized in another strain of S. aureus. In wound and murine abscess infection models, the putP mutant was approximately 10-fold more attenuated than was wild-type strain RN6390. Another S. aureus strain transduced with the putP mutation also displayed an attenuated phenotype after passage in the wound model. A [3H]proline uptake assay showed that less proline was specifically transported into the putP mutant than into strain RN6390. The reduced viability of the bacteria possessing the mutation in the S. aureus high-affinity proline permease suggests that proline scavenging by the bacteria is important for in vivo growth and proliferation and that analogs of proline may serve as potential antistaphylococcal therapeutic agents. 相似文献
73.
Cyanobacterial neurotoxins have been implicated in animal deaths resulting from drinking contaminated water. Anatoxin-a (AN) and homoanatoxin-a (HMAN) have previously been analysed using high-performance liquid chromatography (HPLC) with UV detection, but this procedure is insufficiently sensitive and is subject to interferences. A sensitive fluorimetric (FL) method for determining AN was recently developed using derivatisation with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) and this has been applied to the simultaneous determination of AN, HMAN and their epoxy and dihydro degradation products. Microscale syntheses were used to prepare the dihydro and epoxy derivatives from AN and HMAN. These compounds were produced in high yields, as confirmed by electrospray MS and HPLC-FL of their benzoxadiazole derivatives. All six NBD derivatives were readily separated using isocratic reversed-phase HPLC. The recoveries of these compounds from spiked water samples, using weak cation-exchange (WCX) solid-phase extraction (SPE), were 83.2-84.9% at concentrations of 10 micrograms/l. The R.S.D. values were 1.7-3.9% (n = 8) and the limits of detection were better than 10 ng/l for all six compounds, illustrating the high sensitivity of the method. This methodology was successfully applied to the analysis toxin degradation products in natural samples. Dihydroanatoxin-a (0.8 mg/g) was isolated from a benthic Oscillatoria bloom from Caragh Lake, Ireland, and was found to contain two isomers but their ratio was different from that found in the synthetic material. 相似文献
74.
Robert L. Glass 《Information Systems Management》1995,12(2):67-69
It is the year 2020. The effects of the Date Wars at the turn of the century— in fact, the turn of the millennium— have been mostly absorbed. Has the IS community learned from the past? 相似文献
75.
76.
The steady state diffusion cell test is often used to determine the chloride ion diffusion coefficient in cementitious materials. It involves the measurement of the flux of chloride ions through a specimen under near steady state conditions. It has been noted that such a test may also provide data which characterises the chloride binding capacity of the specimen. In this work a numerical model of chloride diffusion subject to the effects of chloride binding is used to assess the effect of deviations from the steady state on the data obtained from a diffusion cell test. It is noted that there will be a tendency to underestimate the diffusion coefficient, although good practice should limit this error. The predicted error in the chloride binding isotherm is smaller than that in the diffusion coefficient. Furthermore, the influence of errors in the effective porosity on model predictions is limited as the resulting effect on the values of the calculated parameters describing chloride diffusion and binding counteract each other. 相似文献
77.
An inexpensive modification to a gas chromatography injector liner is reported that facilitates continuous admission of analyte into a gas chromatograph/mass spectrometer (GC/MS) for methods development. The MS methods development liner can be made by making simple modifications to commercially available liners and fits into standard injectors in place of the normal liners without any need to break vacuum in the MS. The injector temperature and gas flow rates are adjusted to provide appropriate analyte levels in the MS, which can be admitted under conditions identical with those of real analyses, including co-admission of column bleed. The device is particularly useful for development of tandem MS methods in GC/MS/MS instruments, which are configured with the GC as the sole sample inlet. 相似文献
78.
KA Illig K Ouriel JA DeWeese CK Shortell RM Green 《Canadian Metallurgical Quarterly》1998,163(11):794-796
Controversy exists regarding the indications and methods for lower-extremity fasciotomy. Two recent cases at our institution in which recurrent, acute limb-threatening ischemia occurred despite adequate fascial division have convinced us that in certain situations subcutaneous fasciotomy is clearly inadequate. In both patients, both of whom were young, intact healthy skin between the lower extent of the incision and the malleolus acted as a tourniquet, causing recurrent compartment syndrome as reperfusion edema occurred after initial repair. We believe that therapeutic fasciotomy in young patients with relatively noncompliant skin should include division of skin from the knee to the ankle on at least one side to prevent a tourniquet effect by intact skin at the ankle. 相似文献
79.
This paper presents a new approach to adaptive motion control of an important class of robotic systems. The control schemes developed using this approach are very simple and computationally efficient since they do not require knowledge of either the mathematical model or the parameter values of the robotic system dynamics. It is shown that the control strategies are globally stable in the presence of bounded disturbances, and that the size of the tracking errors can be made arbitrarily small. The proposed controllers are very general and are implementable with a wide variety of robotic systems, including both open- and closed-kinematic-chain manipulators. Computer simulation results are given for a seven degree-of-freedom (DOF) Robotics Research Corporation Model K-1607 arm. These results demonstrate that accurate and robust trajectory tracking can be achieved by using the proposed schemes. 相似文献