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排序方式: 共有2871条查询结果,搜索用时 15 毫秒
81.
Mette B. Petersen Jeanette Pedersen Dinah L. Holm Matthew Denwood Liza R. Nielsen 《Journal of dairy science》2018,101(8):7383-7396
Mycoplasma bovis is an important pathogen causing disease and substantial economic losses in cattle. However, knowledge of the dynamics of antibody responses in individual cows in the face of an outbreak is currently extremely limited. The use of commercial antibody tests to support clinical decision-making and for surveillance purposes is therefore challenging. Our objective was to describe the dynamics of M. bovis antibody responses in 4 Danish dairy herds experiencing an acute outbreak of M. bovis-associated disease, and to compare the antibody dynamics between dairy cows with different disease manifestations. A total of 120 cows were examined using a standardized clinical protocol and categorized into 4 disease groups: “mastitis,” “systemic,” “nonspecific,” and “none.” Paired blood and milk samples were collected and tested using a commercial M. bovis antibody–detecting ELISA. Plots of raw data and generalized additive mixed models with cow and herd as random effects were used to describe serum and milk antibody dynamics relative to the estimated time of onset of clinical disease. Cows with mastitis had high optical density measurement (ODC%) of antibodies in both milk and serum at disease onset. The estimated mean ODC% in milk was below the manufacturer's cut-off for the other groups for the entire study period. The estimated mean serum ODC% in the “systemic” group was high at onset of disease and stayed above the cut-off until 65 d after disease onset. However, the lower 95% confidence interval (CI) for the mean ODC% was only above the manufacturer's cut-off between 7 and 17 d after onset of disease. The CI of the “systemic” and “none” groups did not overlap at any time between the day of disease onset and 65 d after disease onset, and the estimated mean ODC% for both the “nonspecific” and “none” groups were generally below the cut-off for the majority of the study period. In conclusion, the serum antibody responses were highly dynamic and showed a high level of variation between individual cows. This strongly suggests that serology is unlikely to be useful for individual diagnosis of M. bovis-associated disease in dairy cows. However, it might still be useful for herd- or group-level diagnosis. Antibodies in milk were only increased in cows with M. bovis mastitis, indicating that milk antibody measurements only have diagnostic utility for cows with mastitis. 相似文献
82.
Lars Peter Andersen Line Nørdam Thomas Joensson Pete Kines Kent J. Nielsen 《Construction Management & Economics》2018,36(1):22-31
The construction industry has one of the highest frequencies of work-related accidents. We examined whether construction workers predominantly identify themselves in terms of their workgroup or in terms of the construction site. In addition, we examined the associations between social identity and safety climate, and how these constructs are associated with work-related accidents. The analyses were based on questionnaire responses from 478 construction workers from two large construction sites, and the methods involved structural equation modeling. Results showed that the workers identified themselves primarily with their workgroup, and to a lesser degree with the construction site. Social identity and safety climate were related both at the workgroup and construction site levels, meaning that social identity may be an antecedent for safety climate. The association between social identity and safety climate was stronger at the workgroup level than at the construction site level. Finally, safety climate at both levels was inversely associated with self-reported accidents, with the strongest association at the workgroup level. A focus on improving safety climate, particularly by integrating initiatives at both the workgroup and management level, may have the potential to improve safety performance and thus decrease the risk of accidents and injuries on construction sites. 相似文献
83.
Nielsen Guillaume Janin Amelie Coudert Lucie Blais Jean Francois Mercier Guy 《Mine Water and the Environment》2018,37(1):42-55
Mine Water and the Environment - Passive treatment is a promising, green technology that is increasingly being used for mine drainage treatment. However, several challenges remain concerning its... 相似文献
84.
Nielsen Guillaume Coudert Lucie Janin Amelie Blais Jean Francois Mercier Guy 《Mine Water and the Environment》2019,38(1):104-118
Mine Water and the Environment - Acid mine drainage (AMD) is a major environmental challenge for the mining industry in northern climates. Laboratory-scale experiments were conducted to test... 相似文献
85.
Backshoring of production to Western Europe has become an increasingly important trend after decades of offshoring. The subject is introduced by a general discussion followed by a specific analysis of the Nordic surface finishing industry. The main finding is that production quality is the main driver for backshoring of surface finishing. 相似文献
86.
ABSTRACT: Wheat and rye bread artificially inoculated with molds were packed in modified atmospheres of 0%, 50%, 75%, or 100% CO2 balanced with N2 , and 3 levels of residual O2 , 1%, 0.03%, or <0.01%/O2 -absorber, and stored for 30 to 35 d. Modified atmosphere packaging (MAP) was quantitatively more effective for rye bread because fewer mold species grew at elevated CO2 . However, the major rye bread contaminant, Penicillium roqueforti , was the overall most CO2 -resistant mold and only the use of O2 -absorber could prevent growth of this species. On wheat bread, the most CO2 -tolerant mold was Penicillium commune , growing in 99% CO2 (with high residual O2 ), and Aspergillus flavus was the mold species that grew at lowest O2 in 75% CO2 treatment. The spoilage yeast/"chalk mold" Endomyces fibuliger was less affected by the different O2 levels than the true filamentous molds, and none of the tested MAP treatments could prevent growth, but lag-phase was increased with O2 -absorber on wheat bread and decreased with 1% residual O2 on rye bread. Experiments with volatile mustard oil showed that A. flavus and Eurotium repens were the most mustard oil-resistant species on wheat and rye bread, respectively. A combination strategy with MAP and mustard oil proved most optimal, and total inhibition was achieved with 2 μL mustard oil/rye bread slice and between 2 and 3 μL/wheat bread. Results indicated that the nature and surface area of the product influences effectiveness of active packaging with mustard oil. 相似文献
87.
Buitenhuis AJ Lund MS Thomasen JR Thomsen B Nielsen VH Bendixen C Guldbrandtsen B 《Journal of dairy science》2007,90(1):472-481
Lameness is an important factor for culling animals. Strong legs and feet improve herd life of dairy cows. Therefore, many countries include leg and feet conformation traits in their breeding programs, often as early predictors of longevity. However, few countries directly measure lameness related traits to include these in a breeding program. Lameness indices in 3 different lactations and 5 leg conformation traits (rear legs side view, rear legs rear view, hock quality, bone quality, and foot angle) were measured on granddaughters of 19 Danish Holstein grandsires with 33 to 105 sons. A genome scan was performed to detect quantitative trait loci (QTL) based on the 29 autosomes using microsatellite markers. Data were analyzed across and within families for QTL affecting lameness and leg conformation traits. A regression method and a variance component method were used for QTL detection. Two QTL each for lameness in the first [Bos taurus autosome (BTA); BTA5, BTA26] and second (BTA19, BTA22) lactations were detected. For the 5 different leg conformation traits, 7 chromosome-wise significant QTL were detected across families for rear legs side view, 5 for rear legs rear view, 4 for hock quality, 4 for bone quality, and 1 for foot angle. For those chromosomes where a QTL associated with 2 different traits was detected (BTA1, BTA11, BTA15, BTA26, and BTA27), a multitrait-1-QTL model and a multitrait-2-QTL model were performed to characterize these QTL as single QTL with pleiotropic effects or distinct QTL. 相似文献
88.
89.
DR Bielenberg CD Bucana R Sanchez CK Donawho ML Kripke IJ Fidler 《Canadian Metallurgical Quarterly》1998,111(5):864-872
We determined whether cutaneous angiogenesis induced by exposure of mice to ultraviolet-B (UVB) radiation is associated with an imbalance between positive and negative angiogenesis-regulating molecules. Unshaved C3H/HeN mice were exposed to a single dose (15 kJ per m2) of UVB. At various times, the mice were killed, and their external ears were processed for routine histology and immunohistochemistry. Antibodies against proliferating cell nuclear antigen and bromodeoxyuridine identified dividing cells. Antibodies against CD31/ PECAM-1 identified endothelial cells, and antibodies against basic fibroblast growth factor (bFGF), vascular endothelial growth factor/vascular permeability factor, and interferon-beta (IFN-beta) identified angiogenesis-regulating molecules. Epidermal hyperplasia was documented by 48 h and reached a maximum on day 7 after exposure to UVB. The expression of bFGF increased by 24 h, whereas the expression of IFN-beta decreased by 72 h after exposure to UVB. The expression of vascular endothelial growth factor/vascular permeability factor increased slightly after irradiation. The altered balance between bFGF and IFN-beta was associated with increased endothelial cell proliferation (bromodeoxyuridine + CD31 + cells) within existing blood vessels, leading to telangiectasia and new blood vessels. UV-induced epidermal hyperplasia and cutaneous angiogenesis were highest in IFN-alpha/beta receptor knockout mice. These results demonstrate that in response to UVB radiation, dividing keratinocytes produce a positive angiogenic molecule (bFGF) but not a negative angiogenic molecule (IFN-beta), and that this altered balance is associated with enhanced cutaneous angiogenesis. 相似文献
90.
Long-range correlation properties of coding and noncoding DNA sequences: GenBank analysis 总被引:1,自引:0,他引:1
SV Buldyrev AL Goldberger S Havlin RN Mantegna ME Matsa CK Peng M Simons HE Stanley 《Canadian Metallurgical Quarterly》1995,51(5):5084-5091
An open question in computational molecular biology is whether long-range correlations are present in both coding and noncoding DNA or only in the latter. To answer this question, we consider all 33301 coding and all 29453 noncoding eukaryotic sequences--each of length larger than 512 base pairs (bp)--in the present release of the GenBank to dtermine whether there is any statistically significant distinction in their long-range correlation properties. Standard fast Fourier transform (FFT) analysis indicates that coding sequences have practically no correlations in the range from 10 bp to 100 bp (spectral exponent beta=0.00 +/- 0.04, where the uncertainty is two standard deviations). In contrast, for noncoding sequences, the average value of the spectral exponent beta is positive (0.16 +/- 0.05) which unambiguously shows the presence of long-range correlations. We also separately analyze the 874 coding and the 1157 noncoding sequences that have more than 4096 bp and find a larger region of power-law behavior. We calculate the probability that these two data sets (coding and noncoding) were drawn from the same distribution and we find that it is less than 10(-10). We obtain independent confirmation of these findings using the method of detrended fluctuation analysis (DFA), which is designed to treat sequences with statistical heterogeneity, such as DNA's known mosaic structure ("patchiness") arising from the nonstationarity of nucleotide concentration. The near-perfect agreement between the two independent analysis methods, FFT and DFA, increases the confidence in the reliability of our conclusion. 相似文献