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71.
Mouse pancreatic islet grafts under the kidney capsule of syngeneic hosts were removed and perifused in vitro 1-40 weeks after the transplantation. In comparison with fresh islets, 12- to 40-week-old grafts exhibited an attenuated first phase of glucose-stimulated insulin release. In grafts 1, 12, 28, or 40 weeks old, but not in fresh islets, the mean secretory rate during the initial 10 min of stimulation was significantly lower than that during the subsequent 15 min. When expressed in relation to insulin content, the insulin output in response to 11 mmol/L glucose was no less from grafts than from fresh islets; in grafts 12 or 40 weeks old at 16.7 mmol/L glucose, the fractional output above baseline was significantly diminished during the initial 10 min, but not subsequently. Immediately on switching from basal to stimulatory glucose concentration, there was a transient drop in insulin secretion from the grafts, especially after more than 12 weeks of transplantation and in response to 16.7, as compared with 11, mmol/L glucose. When glucose was switched back from stimulatory to basal concentration, grafts also frequently exhibited a transient increase in the insulin secretory rate. Neither initial drops nor "off responses" were seen in untransplanted islets. The modifications of the secretory dynamics in islet grafts suggest that transplantation influences the balance between the stimulatory and inhibitory influences of glucose on the beta-cell's secretory machinery. 相似文献
72.
73.
M Pazianas M Zaidi CL Huang BS Moonga VS Shankar 《Canadian Metallurgical Quarterly》1993,192(3):1100-1105
We demonstrated previously that osteoclasts possess a divalent cation-sensitive "receptor", the Ca2+ receptor. Activation of the Ca2+ receptor by the surrogate cation Ni2+ was shown to elicit an increase in cytosolic [Ca2+] to a peak value followed by an exponential decline. In the present study we examined the influence of surface membrane voltage on the kinetics of Ca2+ receptor inactivation. The K+ ionophore, valinomycin was applied to intercept the declining phase of the cytosolic [Ca2+] transient elicited by application of between 50 microM- and 5 mM-[Ni2+]. This resulted in a sustained elevation of cytosolic [Ca2+] or even a 'hump' followed by a gradual decline. Such a kinetic alteration persisted in a Ca(2+)-free solution, but was abolished in high extracellular [K+] (105 mM). Thus, we demonstrate for the first time to our knowledge, a modulatory effect of membrane potential on the function of the osteoclast Ca2+ receptor. 相似文献
74.
75.
W.CL 《军民两用技术与产品》2005,(2):21
全球领先的特种玻管制造商——德国肖特集团新近开发出两种新型玻璃.用于满足纯平液晶电视显示器(LCD—TFT)的市场需求。这两种新型玻璃8688(Tungsten型)和8271(KOVAR型)是专为纯平液晶电视显示器而开发的。 相似文献
76.
Lightning continues to be the major cause of outages on overhead power distribution lines. Through laboratory testing and field observations and measurements, the properties of a lightning stroke and its effects on electrical distribution system components are fairly well-understood phenomena. A meticulous compilation of 32 years of historical records has been kept for outage causes, duration, and locations for eight distribution feeders at the Oak Ridge National Laboratory (ORNL). Due to the limited growth of ORNL, the number, length, and location of the 13.8 kV overhead lines have remained the same between 1960 and 1992. Except for noted differences (voltage construction class, length, age, and maximum elevation above a reference level), other factors that could influence the reliability of an overhead line have remained nearly the same. This allowed a meaningful reliability study to be performed on the entire ORNL electrical distribution system. In this article, the main findings of the reliability assessment as it relates to lightning-resistant overhead line construction techniques are out-lined, and a simple and cost-effective method to reduce lightning caused outages is offered. In addition, comparisons are made between the failure rates and causes experienced at ORNL and those in industry surveys. Where large discrepancies exist between survey data and experiences at ORNL, evidence is presented to explain the differences between ORNL's distribution system and those typical of industry 相似文献
77.
William S. Cleveland 《技术计量学》2013,55(4):713-723
The mathematical theory of the best linear prediction of stationary time series presumes that the model generating the series, which can be specified by either the autocovariance function or the spectral density, is known. The true model is, of course, not known in practice, and the procedure is to fit a model and predict as if this fitted model were the truth. The question then is one of deciding whether the resulting predictions are about as good as could be gotten if the truth were known. This paper describes a method for assessing the predictions of the fitting model by an analysis of residuals. In particular, it is argued that the traditional tests of hypothesis for white noise are inappropriate if prediction is the goal, and a method is described for determining whether or not the mean square errors of the predictions arising from the fitted model can be measurably reduced. 相似文献
78.
Cleveland Pablo Rios Sebastian A. Aguilera Felipe Graña Manuel 《Neural computing & applications》2022,34(19):16717-16738
Neural Computing and Applications - Understanding at microscopic level the generation of contents in an online social network (OSN) is highly desirable for an improved management of the OSN and the... 相似文献
79.
DJ McKenzie G Piraccini A Felskie P Romano P Bronzi CL Bolis 《Canadian Metallurgical Quarterly》1999,72(1):116-125
Nile tilapia (Oreochromis niloticus) were infused with ammonium salts, acid, and base to investigate the effects of changes in arterial plasma total ammonia content (Tamm) and pH (pHa) on plasma urea-nitrogen (urea-N) levels and urea-N excretory fluxes (Jurea-N). The tilapia did not possess a functional hepatic ornithine urea-cycle (no significant carbamyl phosphate synthetase III activity). Infused substances were dissolved in a saline vehicle and injected twice (5 mL kg-1), the first infusion to "prime" the animal and promote a more marked response to the second infusion, given 2.5 h later. The results reported are those of the second infusion. Infusion of 200 mM NH4Cl increased Tamm, reduced pHa, and increased plasma urea-N and Jurea-N. Two hundred mM NH4HCO3 increased Tamm and arterial plasma total CO2 content (TaCO2), reduced pHa, and increased Jurea-N. Fifty mM HCl reduced pHa but had no effects on urea dynamics. Fifty mM NaOH increased pHa, plasma urea-N levels, and Jurea-N. Two hundred mM NaHCO3 increased pHa, TaCO2, plasma urea-N levels, and Jurea-N. Infusion of the saline vehicle was without effect. The results indicate that ammonia loading and plasma alkalosis both stimulate urea excretion in uricolytic fish. The responses to hyperammonemia or alkalosis were not modified when combined with elevated plasma bicarbonate levels. 相似文献
80.
An approximately 60-kb transferable, vanB-carrying plasmid has been identified in a clinical Enterococcus faecium strain. A similar plasmid has been observed in an unrelated E. faecium strain, suggesting that plasmid transfer of vanB operons occurs in nature and plays a role in the dissemination of VanB-type resistance among strains of E. faecium. 相似文献