Early onset of parturition induced by acute alcohol exposure in C57BL/6J mice: role of uterine PGE and PGF2alpha

These studies were designed to determine the effect of acute alcohol treatment on gestational length and to probe for a mechanism underlying alcohol-induced early onset of parturition (EOP) in mice. Experiment 1: alcohol increases the incidence of EOP. Pregnant C57BL/6J mice were given alcohol (0, 4, 5 or 6 g kg(-1), i.g.) on Gestational Day (GD) 10, 15, 16, 17 or 18. Deliveries were monitored every 6 h from GD 18. Results indicated that 6 g kg(-1) alcohol treatment on GD 17 or 18 increased the incidence of EOP. Experiment 2: prostaglandins (PGs) play roles in parturition. The purpose of Experiment 2 was to determine whether PGs mediate alcohol-induced EOP in mice. The results indicated that pretreatment on GD 17 with aspirin, a prostaglandin synthesis inhibitor, prevented alcohol-induced EOP. These data suggest that alcohol-induced EOP in mice may be mediated by PGs. Experiment 3: PGs are influenced by alcohol and are triggers of labour. Experiment 3 measured uterine PGs associated with the onset of alcohol-induced EOP in mice. Alcohol increased uterine PGE and PGF2alpha, with PGE levels higher than control before labour, and elevated PGF2alpha levels correlating with labour. Changes in gestational length have important implications for pregnancy outcome, as well as for normal fetal growth and development.     

AN ANALYSIS OF CRACK TIP SHIELDING IN ALUMINUM ALLOY 2124: A COMPARISON OF LARGE, SMALL, THROUGH-THICKNESS AND SURFACE FATIGUE CRACKS

Abstract— The development of crack closure during the plane strain extension of large and small fatigue cracks has been investigated in a 2124 aluminum alloy using both experimental and numerical procedures. Specifically, the growth rate and crack closure behavior of long (∼17–38 mm) cracks, through-thickness physically-short (50–400 μm) cracks, and naturally-occurring microstructurally-small (2–400 μm) surface cracks have been examined experimentally from threshold levels to instability (over the range 10–^12–10–⁶m/cycle). Results are compared with those predicted numerically using an elastic-plastic finite element analysis of fatigue crack advance and closure under both plane stress and plane strain conditions. It is shown that both the short through-thickness and small surface cracks propagate below the long crack threshold at rates considerably in excess of long cracks, consistent with the reduced levels of closure developed in their limited wake. Numerical analysis, however, is found consistently to underpredict the magnitude of crack closure for both large and small cracks, particularly at near-threshold levels; an observation attributed to the fact that the numerical procedures can only model contributions from cyclic plasticity, whereas in reality significant additional closure arises from the wedging action of fracture surface asperities and corrosion debris. Although such shielding mechanisms are considered to provide a prominent mechanism for differences in the growth rate behavior of large and small cracks, other factors such as the nature of the stress and strain singularity and the extent of local plasticity are shown to play an important role.