全文获取类型
收费全文 | 2800篇 |
免费 | 7篇 |
专业分类
电工技术 | 13篇 |
综合类 | 1篇 |
化学工业 | 60篇 |
金属工艺 | 28篇 |
机械仪表 | 6篇 |
建筑科学 | 43篇 |
能源动力 | 10篇 |
轻工业 | 71篇 |
水利工程 | 7篇 |
石油天然气 | 1篇 |
无线电 | 31篇 |
一般工业技术 | 83篇 |
冶金工业 | 2403篇 |
原子能技术 | 11篇 |
自动化技术 | 39篇 |
出版年
2022年 | 6篇 |
2021年 | 4篇 |
2019年 | 9篇 |
2018年 | 5篇 |
2017年 | 8篇 |
2016年 | 4篇 |
2014年 | 12篇 |
2013年 | 26篇 |
2012年 | 18篇 |
2011年 | 23篇 |
2010年 | 14篇 |
2009年 | 10篇 |
2008年 | 17篇 |
2007年 | 18篇 |
2006年 | 16篇 |
2005年 | 10篇 |
2004年 | 19篇 |
2003年 | 13篇 |
2002年 | 14篇 |
2001年 | 5篇 |
2000年 | 11篇 |
1999年 | 89篇 |
1998年 | 829篇 |
1997年 | 423篇 |
1996年 | 268篇 |
1995年 | 162篇 |
1994年 | 116篇 |
1993年 | 156篇 |
1992年 | 26篇 |
1991年 | 29篇 |
1990年 | 32篇 |
1989年 | 28篇 |
1988年 | 32篇 |
1987年 | 49篇 |
1986年 | 27篇 |
1985年 | 19篇 |
1984年 | 3篇 |
1983年 | 10篇 |
1982年 | 6篇 |
1981年 | 13篇 |
1980年 | 34篇 |
1979年 | 5篇 |
1978年 | 7篇 |
1977年 | 38篇 |
1976年 | 107篇 |
1975年 | 6篇 |
1973年 | 4篇 |
1971年 | 3篇 |
1927年 | 2篇 |
1919年 | 2篇 |
排序方式: 共有2807条查询结果,搜索用时 15 毫秒
31.
RM Torres CL Law L Santos-Argumedo PA Kirkham K Grabstein RM Parkhouse EA Clark 《Canadian Metallurgical Quarterly》1992,149(8):2641-2649
The human B lymphocyte-specific Ag, CD22, is a cell adhesion molecule expressed on the surface during a narrow window of B cell development, coincident with surface IgD. A ligand for CD22 has recently been identified on human T cells as the low molecular mass isoform of the leukocyte common Ag, CD45RO. CD22 has been reported to function in the regulation of both T and B cell activation in vitro. In this study, we report the isolation and expression of a molecular cDNA clone encoding the murine homologue of CD22, mCD22. Within their predicted protein sequences, murine and human sequences overall have 62% identity, which includes 18 of 20 extracellular cysteines and six of six cytoplasmic tyrosines. BHK cells transfected with mCD22 cDNA specifically adhere to resting and activated T lymphocytes and in addition bound activated, but not resting, B cells. Five Th clones were analyzed for their ability to adhere to mCD22; two Th0 clones and one Th1 clone bound CD22+ BHK transfectants, but not all T cell clones bound CD22+ cells: another Th1 clone and a Th2 clone did not. mCD22+ BHK transfectants were also specifically bound by the B cell-specific mAb, NIM-R6, demonstrating that this mAb is specific for murine CD22. Human cell lines expressing the counter-receptors for human CD22 were also examined for adhesion to the murine CD22 homologue; the epitope responsible for B cell adhesion to CD22 is conserved, whereas the T cell epitope binding to CD22 is not. The cDNA and mAb to murine CD22 will be useful for defining the in vivo function of CD22. 相似文献
32.
J Buc CL Santini F Blasco R Giordani ML Cárdenas M Chippaux A Cornish-Bowden G Giordano 《Canadian Metallurgical Quarterly》1995,234(3):766-772
A soluble alpha beta complex of nitrate reductase can be obtained from a strain of Escherichia coli that lacks the narI gene and expresses only the alpha and beta subunits. The beta subunit contains four Fe-S centres and the alpha subunit contains the molybdenum cofactor, which is the site at which nitrate is reduced. Despite the lack of the gamma subunit of the complete enzyme, this complex can still catalyse the reduction of nitrate with artificial electron donors such as benzyl viologen, so that it is suitable for studying the transfer of electrons between these two types of redox centre. To examine whether the electrons from reduced benzyl viologen are initially delivered to the Fe-S centres, or directly to the molybdenum cofactor, or both, we have studied the steady-state kinetics and the binding of benzyl viologen to the alpha beta complex and mutants alpha beta* with altered beta subunits. Reduction of the enzyme by reduced benzyl viologen in the absence of nitrate showed that all four Fe-S centres and the molybdenum cofactor could be reduced. Two classes of site with different equilibrium constants could be distinguished. The kinetic results suggest that benzyl viologen supplies its electrons directly to the molybdenum cofactor, at a rate showing a hyperbolic dependence on the square of the concentration of the electron donor. A reaction mechanism is proposed for the reduction of nitrate catalysed by the alpha beta complex of nitrate reductase with artificial electron donors. 相似文献
33.
34.
35.
36.
37.
Hofsajer I.W. Ferreira J.A. van Wyk J.D. Holm M.F.K. 《Industry Applications Magazine, IEEE》1995,1(5):24-29
In order to reduce the cost, size, and weight of power electronic systems, it has become necessary to integrate electromagnetic structures, which until now have been constructed with discrete components. This approach not only reduces the component count, but also gives much greater control over parasitic elements. In this article, the authors describe an electromagnetically integrated resistor-capacitor-diode (RCD) snubber/voltage clamp that uses a planar construction technique. The design and construction are described and the performance is verified experimentally. Some advantages of the integrated component over its discrete counterpart are also given 相似文献
38.
P Belan J Gardner O Gerasimenko J Gerasimenko CL Mills OH Petersen AV Tepikin 《Canadian Metallurgical Quarterly》1998,273(7):4106-4111
Secretory cells should in principle export substantial amounts of calcium via exocytosis since Ca2+ is sequestered in secretory granules. Based on a new technique for measurements of the extracellular calcium concentration in the vicinity of the cell membrane and on the droplet technique, we have monitored the rate of calcium extrusion from salivary gland acinar cells. Isoproterenol (ISP), a beta-adrenergic agonist and powerful secretogogue, evoked no change in the cytosolic free Ca2+ concentration ([Ca2+]i) but induced vigorous extracellular Ca2+ concentration ([Ca2+]i) spiking. The absence of [Ca2+]i elevation and the pulsatile nature of the changes in [Ca2+]i indicate that these spikes are most likely due to calcium release from secretory granules. The cholinergic agonist acetylcholine (ACh), which induces moderate secretion, evoked a marked rise in [Ca2+]i and a smooth rise in [Ca2+]i, most likely induced by plasma membrane calcium pumps, on which shortlasting [Ca2+]i spikes were superimposed. The rate of ISP-induced calcium efflux was very substantial. The calculated calcium loss during the first 100 s of supramaximal stimulation corresponded to a reduction of the total cellular calcium concentration of approximately 0.4 mM. We conclude that in salivary glands, calcium release via exocytosis is one of the main mechanisms extruding calcium from cells to the extracellular milieu. 相似文献
39.
MJ Concannon B Gainor GF Petroski CL Puckett 《Canadian Metallurgical Quarterly》1997,100(6):1452-1458
In recent years, electrodiagnostic studies have become an expected component in the work up and evaluation of carpal tunnel syndrome. We conducted a retrospective review of 460 carpal tunnel decompressions to determine whether the accuracy of diagnosis and the prediction of therapeutic outcome could be related to the positivity and severity of findings on preoperative electrical studies. The 349 patients (460 hands) were divided into two groups: group 1 consisted of hands with the clinical diagnosis of carpal tunnel syndrome but with normal electrodiagnostic studies (n = 62); in group 2 the hands had a clinical diagnosis of carpal tunnel syndrome with confirmatory electrodiagnostic studies (n = 398). The number and distribution of signs and symptoms of carpal tunnel syndrome were not statistically different between these two groups. There was not a statistically significant difference in the success rate of surgery or the incidence of complications. The similarities between these two groups suggests that the distinction between them (the positivity of electrodiagnostic studies) is an artificial one and that the clinical diagnosis of carpal tunnel syndrome is sufficient to predict the presence of the disease, as well as outcome of surgery. On the basis of these data, strict adherence to electrodiagnostic studies to confirm the diagnosis will exclude 13 percent of the patients with legitimate carpal tunnel syndrome from receiving appropriate therapy. 相似文献
40.
FT Hakim R Cepeda S Kaimei CL Mackall N McAtee J Zujewski K Cowan RE Gress 《Canadian Metallurgical Quarterly》1997,90(9):3789-3798
To examine the mechanisms of CD4 reconstitution in an adult population, lymphocyte repopulation was assessed following dose-intense chemotherapy in 25 breast cancer patients, ages 33 to 69 years. Chemotherapy resulted in a greater than 60% reduction in total CD4 T cells and, in particular, a greater than 90% loss of the CD45RA+ CD4 cells. CD4 recovery was protracted, achieving less than 50% of pretreatment levels after 12 to 14 months. Two facets of the CD4 recovery were notable. First, generation of CD45RA+ CD4 cells played only a minor role in the first year, suggesting that thymic production was not the main route of CD4 regeneration. Indeed, recovery of CD45RA+ CD4 cell levels remained limited in half of the patients even after 2 years. Second, expansion of the mature peripheral CD4 cells (CD45RO+) remaining after chemotherapy was the main source of early CD4 repopulation, peaking at 3 to 6 months postchemotherapy. This expansion was limited in duration, however, and was followed by a secondary decline, such that the total CD45RO+ CD4 levels at 9 to 12 months were lower than at 6 months. When stimulated by mitogens, an increased susceptibility to apoptosis was observed in postchemotherapy CD4 cells as compared with those from normal donors. The elevated expression of markers such as HLA-DR during chemotherapy and for several months postchemotherapy is consistent with the presence of an activated T-cell population. CD4 apoptotic frequency correlated with the frequency of HLA-DR expression on T cells. Thus, CD4 recovery is constrained in adults by a limited thymic regenerative capacity and by an increased susceptibility to apoptosis within the expanding peripheral CD4 population. 相似文献