Small structural changes of chromosome 8. Two cases with evidence for deletion

The degeneration of the substantia nigra that characterises Parkinson's disease may cause an alteration in sensitivity of striatal dopamine receptors. The development of denervation supersensitivity has been held to be responsible for some of the effects of chronic levodopa therapy. The rotating rodent is an animal model commonly used to study the phenomenon of striatal dopamine receptor supersensitivity, and to investigate drugs which may prove to be beneficial in the treatment of Parkinson's disease. We have investigated as to whether long-term oral administration of levodopa to mice with unilateral destruction of striatal dopaminergic nerve terminals influences dopaminergic receptor denervation supersensitivity as judged by the circling response following systemically administered levodopa. It does not do so and the relevance of these findings to the treatment of Parkinson's disease is discussed.     

Analysis of three restriction fragment length polymorphisms in the human type II procollagen gene

Cloned genomic DNA sequences corresponding to various regions of the human type II procollagen gene were used to analyze the DNA from 78 normal volunteers. Southern hybridization experiments detected polymorphic HindIII, BamHI, and EcoRI sites. The presence of the polymorphic HindIII site results in a 7.0-kilobase (kb) band, and the absence of this site results in a 14.0-kb band. When present, the BamH1 polymorphic site yields a 4.8-kb band, and when absent, yields a 7.2-kb band. The presence of the EcoRI polymorphic site results in a 3.7-kb band, and its absence results in a 7.0-kb band. Each polymorphic site was mapped. Analyses of the data demonstrated that the sites are present in overall gene frequencies of .39 for HindIII, .04 for BamHI, and .02 for EcoRI. Gene frequencies of the polymorphic sites were also studied with respect to race. The polymorphic sites are present in a Hardy-Weinberg distribution in the study population. Study of an extended family demonstrated that the segregation of the HindIII polymorphic site is consistent with Mendelian inheritance.     

Complex Detection: A Waveform Preserving Technique for Single-Sideband Demodulation

The full-carrier single-sideband signal is of the minimal phase type with limited phase excursion and contains sufficient information to recover completely the message waveform. A receiving system is described that makes use of both the envelope and the phase information in the signal. Operation is independent of input signal level and requires no wide-band quadrature network.     

New feasibilities for characterizing rough surfaces by optical-correlation techniques

New feasibilities are considered for the optical-correlation diagnostics of rough surfaces with different distributions of irregularities. The influence of deviations of the height surface roughness distribution from a Gaussian probability distribution on the accuracy of optical analysis is discussed. Possibilities for the optical diagnostics of fractal surface structures are shown, and a set of statistical and dimensional parameters of the scattered fields for surface roughness diagnostics is determined. Finally, a multifunctional measuring device for estimating these parameters is proposed.     

CO(2) Imaging with Saturated Planar Laser-Induced Vibrational Fluorescence

We present new vibrational (infrared) planar laser-induced fluorescence (PLIF) imaging techniques for CO(2) that use a simple, inexpensive, high-pulse-energy transversely excited atmospheric CO(2) laser to saturate a CO(2) absorption transition at 10.6 mum. Strong excitation by use of a CO(2) laser provides increased signal levels at flame temperatures and simplifies image interpretation. Because rotational energy transfer and intramodal vibrational energy transfer are fast, vibrational distributions can be approximated by use of a simple three-temperature model. Imaging results from a 425 K unsteady transverse CO(2) jet and a laminar coflowing CO/H(2) diffusion flame with temperatures near 1500 K are presented. If needed, temperature-insensitive signal levels can be generated with a two-laser technique. These results illustrate the potential for saturated infrared PLIF in a variety of flows.     

Cognitive and learning difficulties and how they affect access to IT systems

In October 2005, the IBM Human Ability and Accessibility Center and T.J. Watson Research Center hosted a symposium on “cognitive and learning difficulties and how they affect access to IT systems”. The central premise of the symposium was the recognition that cognitive and learning difficulties have a profound impact on a person’s ability to interact with information technology (IT) systems, but that little support is currently being offered by those systems. By bringing together internationally renowned experts from a variety of different, but complementary, research fields, the symposium aimed to provide a complete overview of the issues related to this topic. This paper summarises the discussions and findings of the symposium.     

Evaluating the complementarity of acoustic and satellite remote sensing for seagrass landscape mapping

Accurate maps representing seagrass spatial distribution are essential components for effective monitoring and management of coastal vegetated habitats. Satellite and acoustic remote sensing provide valuable spatial data for seagrass mapping, though few studies have evaluated the complementarity of these methods. In this study, the complementarity of seagrass mapping was assessed through comparison of acoustic and satellite remote-sensing data sets. QuickBird^® satellite imagery representing the seagrass landscape of the Richibucto estuary, New Brunswick, Canada, was classified through an object-based procedure and evaluated against a single-beam sonar data set. Acoustic percentage cover values were classified into binary presence/absence format through the application of a decision threshold, allowing comparison with satellite data using the error matrix and derived metrics. Though the binary satellite classification resulted in relatively high accuracy compared with independent ground reference data, agreement between satellite and acoustic data sets was limited. Local differences in seagrass prevalence and patchiness affected classification accuracy, highlighting the potential for under- or overestimating seagrass cover when applying bay-scale classification to areas with different landscape structure. These results emphasize the importance of landscape context in seagrass mapping. Satellite and acoustic remote sensing were seen to fundamentally differ in their depiction of the landscape. Comparison of multiple remote-sensing methods allowed for assessment of complementarity as well as ecologically relevant insight to seagrass spatial dynamics, with implications for mapping and monitoring of seagrass habitats.     

Redox control of exofacial protein thiols/disulfides by protein disulfide isomerase

Protein disulfide isomerase (PDI) facilitates proper folding and disulfide bonding of nascent proteins in the endoplasmic reticulum and is secreted by cells and associates with the cell surface. We examined the consequence of over- or underexpression of PDI in HT1080 fibrosarcoma cells for the redox state of cell-surface protein thiols/disulfides. Overexpression of PDI resulted in 3.6-4. 2-fold enhanced secretion of PDI and 1.5-1.7-fold increase in surface-bound PDI. Antisense-mediated underexpression of PDI caused 38-53% decreased secretion and 10-33% decrease in surface-bound PDI. Using 5,5'-dithio-bis(2-nitrobenzoic acid) to measure surface protein thiols, a 41-50% increase in surface thiols was observed in PDI-overexpressing cells, whereas a 29-33% decrease was observed in underexpressing cells. Surface thiol content was strongly correlated with cellular (r = 0.998) and secreted (r = 0.969) PDI levels. The pattern of exofacial protein thiols was examined by labeling with the membrane-impermeable thiol reactive compound, 3-(N-maleimidylpropionyl)biocytin. Fourteen identifiable proteins on HT1080 cells were labeled with 3-(N-maleimidylpropionyl)biocytin. The intensity of labeling of 11 proteins was increased with overexpression of PDI, whereas the intensity of labeling of 3 of the 11 proteins was clearly decreased with underexpression of PDI. These findings indicated that secreted PDI was controlling the redox state of existing exofacial protein thiols or reactive disulfide bonds.