AP Evan  DP Henry  BA Connors  P Summerlin  WH Lee 《Canadian Metallurgical Quarterly》1995,48(5):1517-1529

Immunocytochemistry, in situ hybridization, and radioimmunoassay were employed to examine the cellular distribution of mRNAs and proteins for IGF-I, II, IGF-II/M6P receptor, IGFBP2 as well as the levels of IGF-I and II in normal and unilaterally nephrectomized (Nx) adult rat kidneys. A similar distribution of immunoreactive IGF-I, and -II as well as IGF-II/M6P receptor was found in the principal cells of the cortical collecting duct and in all cells of the inner medullary collecting duct. In addition, immunostainable IGF-I and IGF-II/M6P receptor were noted in some inner medullary loops of Henle, while IGFBP2 was seen in the collecting ducts and loops of Henle of the inner medullar and the renal vasculature of all animals. By comparison, in situ hybridization revealed IGF-I mRNA only in the medullary thick ascending limbs while IGF-II mRNA was localized to the wall of the renal microvasculature in all kidneys. IGFBP2 mRNA was localized to the renal corpuscle and to inner medullary interstitial cells of all kidneys. These data suggest that renal IGF-I and IGFBP2 are synthesized at upstream sites along the nephron and then transported downstream for interaction with IGF receptors. Following nephrectomy, the renal levels of IGF-I peptide and mRNA were elevated at both 5 and 33 days post-nephrectomy, supporting a potential functional role for IGF-I in stimulating the structural and functional recovery in compensatory hypertrophy.  相似文献   

    

MD Henry  C Gonzalez Agosti  F Solomon 《Canadian Metallurgical Quarterly》1995,129(4):1007-1022

The ERM proteins--ezrin, radixin, and moesin--occur in particular cortical cytoskeletal structures. Several lines of evidence suggest that they interact with both cytoskeletal elements and plasma membrane components. Here we described the properties of full-length and truncated radixin polypeptides expressed in transfected cells. In stable transfectants, exogenous full-length radixin behaves much like endogenous ERM proteins, localizing to the same cortical structures. However, the presence of full-length radixin or its carboxy-terminal domain in cortical structures correlates with greatly diminished staining of endogenous moesin in those structures, suggesting that radixin and moesin compete for a limiting factor required for normal associations in the cell. The results also reveal distinct roles for the amino- and carboxy-terminal domains. At low levels relative to endogenous radixin, the carboxy-terminal polypeptide is associated with most of the correct cortical targets except cleavage furrows. In contrast, the amino-terminal polypeptide is diffusely localized throughout the cell. Low level expression of full-length radixin or either of the truncated polypeptides has no detectable effect on cell physiology. However, high level expression of the carboxy-terminal domain dramatically disrupts normal cytoskeletal structures and functions. At these high levels, the amino-terminal polypeptide does localize to cortical structures, but does not affect the cells. We conclude that the behavior of radixin in cells depends upon activities contributed by separate domains of the protein, but also requires modulating interactions between those domains.  相似文献   

    

KW Mahaffey  CB Granger  R Collins  CM O'Connor  EM Ohman  SD Bleich  JJ Col  RM Califf 《Canadian Metallurgical Quarterly》1996,77(8):551-556

Intravenous heparin is routinely given after thrombolytic therapy for patients with acute myocardial infarction in the United States and in some, but by no means all, other countries. Several trials have documented improved infarct-artery patency in patients treated with heparin; however, none was large enough individually to assess the effect of heparin on clinical outcomes. We performed a systematic overview of the 6 randomized controlled trials (1,735 patients) to summarize the available data concerning the risks and benefits of intravenous heparin versus no heparin after thrombolytic therapy. Mortality before hospital discharge was 5.1% for patients allocated to intravenous heparin compared with 5.6% for controls (relative risk reduction of 9%, odds ratio 0.91, 95% confidence interval 0.59 to 1.39). Similar rates of recurrent ischemia and reinfarction were observed among those allocated to heparin therapy or control. The rates of total stroke, intracranial hemorrhage, and severe bleeding were similar in patients allocated to heparin; however, the risk of any severity of bleeding was significantly higher (22.7% vs 16.2%; odds ratio 1.55, 95% confidence interval 1.21 to 1.98). There was no significant difference in the observed effects of heparin between patients receiving tissue-type plasminogen activator and those receiving streptokinase or anisoylated plasminogen streptokinase activator complex, or between patients who did and did not receive aspirin. The findings of this overview demonstrate that insufficient clinical outcome data are available to support or to refute the routine use of intravenous heparin therapy after thrombolysis. It is not known if these findings are due to lack of statistical power, inappropriate levels of anticoagulation, or lack of benefit of intravenous heparin. Large randomized studies of heparin (and of new antithrombotic regimens) are needed to establish the role of such therapy.  相似文献   

    

CM Rao  TB Deb  K Datta 《Canadian Metallurgical Quarterly》1996,40(2):327-337

In this report, the role of 34 kDa HA-binding protein in hyaluronic acid-induced cellular signalling in lymphocytes has been examined. The binding of 125I-HA to lymphocytes in vivo was found to be inhibited by pre-incubation of the cells with anti-34 kDa HA-binding protein antibodies, thus confirming 34 kDa HA-binding protein as the specific HA-receptor in lymphocytes. This observation was substantiated by anti-34 kDa HA-binding protein antibodies immunoblotting and 125I-HA ligand blotting of lymphocytes cell lysate. The HA-induced cell aggregation, tyrosine phosphorylation and cytoskeletal protein phosphorylation demonstrate the HA-induced early cellular signalling events in lymphocytes. Further, to study the involvement of 34 kDa HA-binding protein in mitogen induced lymphocyte signalling, we studied in vivo phosphorylation and secondary messenger formation. The enhanced 34 kDa HA-binding protein phosphorylation by HA and the inhibition of cellular aggregation and IP3 formation by anti-HA-binding protein antibodies revealed that 34 kDa HA-binding protein is one of the potential mediators in HA-induced signal transduction.  相似文献   

    

CM Snapper  F Rosas  MA Moorman  L Jin  K Shanebeck  DM Klinman  MR Kehry  JJ Mond  CR Maliszewski 《Canadian Metallurgical Quarterly》1996,8(6):877-885

IFN-gamma has been shown to either stimulate or inhibit Ig secretion. No studies have yet addressed the basis for these seemingly conflicting properties nor whether IFN-gamma acted directly at the level of the B cell to mediate its effects. Thus, we studied the ability of IFN-gamma to regulate Ig secretion in sort-purified, resting murine B cells that were >99% Ig+, activated either through membrane Ig using unconjugated or dextran-conjugated anti-IgD antibodies (alphadelta-dex) or through CD40 using soluble or membrane CD40 ligand (CD40L). B cells activated with alphadelta-dex proliferated but do not secrete Ig, even in the presence of IL-1 + IL-2. We demonstrate that IFN-gamma only when added subsequent to B cell stimulation with alphadelta-dex, but not unconjugated anti-IfD antibody, plus IL-1 + IL-2 induces up to 100-fold enhancements in Ig secretion and in the numbers of Ig-secreting cells. The predominant Ig isotype secreted is IgM, with IgG3 and IgG2a comprising the majority of non-IgM antibody. IFN-gamma must act in concert with IL-2 for stimulation of Ig secretion. Further, IFN-gamma synergizes with IL-3 + granulocyte-macrophage colony stimulating factor for induction of Ig synthesis. IFN-gamma also enhances IgA syntheses by transforming growth factor-beta-induced membrane IgA+ cells. By contrast, 125IIFN-gamma fails to stimulate Ig secretion in B cells activated with CD40L in the presence or absence of IL-1 + IL-2 or IL-4. However, the combination of CD40L and alphabeta-dex is strongly synergistic for IFN-gamma-induced Ig secretion. Thus, these data establish that IFN-gamma can act directly on the B cell to induce Ig synthesis without the participation of any other cell and demonstrates that the mode of activation of the B cell plays an important role in directing the action of IFN-gamma.  相似文献   

    

M Radmacher  M Fritz  CM Kacher  JP Cleveland  PK Hansma 《Canadian Metallurgical Quarterly》1996,70(1):556-567

We have measured force curves as a function of the lateral position on top of human platelets with the atomic force microscope. These force curves show the indentation of the cell as the tip loads the sample. By analyzing these force curves we were able to determine the elastic modulus of the platelet with a lateral resolution of approximately 100 nm. The elastic moduli were in a range of 1-50 kPa measured in the frequency range of 1-50 Hz. Loading forces could be controlled with a resolution of 80 pN and indentations of the platelet could be determined with a resolution of 20 nm.  相似文献   

    

Henry E. Haxo Jr. 《Geotextiles and Geomembranes》1986,3(4):225-247

The lining of a facility for storing or disposing of hazardous materials or wastes on land must serve as an effective barrier to prevent potential contaminating species from migrating into the ground and the groundwater. The process from the design phase through the manufacture of a polymeric geomembrane to its installation and acceptance as a liner involves many steps that must be controlled in order to assure the ultimate effectiveness of the lining.

In this paper, a program of quality assurance and quality control involved in the critical steps in the process of constructing a lined waste disposal facility from its design through the final acceptance is described. Included are design, geomembrane selection and compatibility testing, specification, raw materials, geomembrane manufacture, panel fabrication, shipment to field site, inspection, installation and performance testing of the lined facility. Emphasis is placed on factors and properties of geomembranes that relate to service performance and must be controlled. The properties include overall thickness, thickness of coatings above threads, composition control, crystallinity, carbon black content, ply adhesion and seam integrity and strength.  相似文献   

    

R Biswas  GS Grest  CM Soukoulis 《Canadian Metallurgical Quarterly》1987,36(14):7437-7441

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Robert H. McKnight  Carol Donnelly  Henry P. Cole  I. J. Ross  Larry R. Piercy 《工程教育杂志》1996,85(4):339-341

A research center in the College of Medicine assisted the Biosystems and Agricultural Engineering Department in the assessment of occupational health and safety instruction in the undergraduate agricultural engineering curriculum. An interdisciplinary team developed a questionnaire to explore three facets of health and safety instruction: 1) content and placement in the curriculum, 2) reasons for teaching occupational health and safety, and 3) adequacy of teaching resources. The questionnaire was pilot tested using agricultural engineering faculty and technicians from a large land grant university. Responses to the questionnaire revealed strengths and weaknesses in the curriculum regarding occupational health and safety instruction. The questionnaire will be expanded and further tested with a broader subject base. This cooperative effort demonstrates that medical schools can be a valuable resource to engineering schools who are looking for ways to improve occupational health and safety instruction in their curricula.  相似文献   

    

Gang‐Won Jang  Henry Panganiban  Tae Jin Chung 《International journal for numerical methods in engineering》2010,84(6):685-707

This investigation focuses on an alternative approach to topology optimization problems involving incompressible materials using the P1‐nonconforming finite element. Instead of using the mixed displacement‐pressure formulation, a pure displacement‐based approach can be employed for finite element formulation owing to the Poisson locking‐free property of the P1‐nonconforming element. Moreover, because the P1‐nonconforming element has linear shape functions that are defined at element vertices, it has considerably fewer degrees of freedom than other quadrilateral nonconforming elements and its implementation is as simple as that of the conforming bilinear element. Various problems dealing with incompressible materials and pressure‐loaded structures found in published works are solved to verify the applicability of the proposed method. The application of the method is extended to the optimal design of fluid channels in the Stokes flow. This is done by expressing pressure in terms of volumetric strain rates and developing a velocity‐field‐only finite element formulation. The optimization results obtained from all the problems considered in this study are in close agreement with those found in the literature. Copyright © 2010 John Wiley & Sons, Ltd.  相似文献