Virulence of Aspergillus fumigatus double mutants lacking restriction and an alkaline protease in a low-dose model of invasive pulmonary aspergillosis

To investigate the pathogenicity of Aspergillus fumigatus mutants lacking putative virulence factors, we have developed a new murine model of invasive pulmonary aspergillosis based on neutropenia, the major factor predisposing patients to this infection. Mice were treated with cyclophosphamide and inoculated by the intranasal route with 5 x 10(3) conidia, a significant reduction from inoculum levels used in previous models. Evidence for the production of the extracellular alkaline protease (Alp) in lung tissue was obtained by using a fungal transformant harboring an alp::lacZ reporter gene fusion. The pathogenicities of single mutant strains lacking either Alp or the ribotoxin restrictocin and of a double mutant strain lacking both proteins were assessed in this infection model. There were no significant differences between the mutant and the wild-type strains in terms of mortality or histological-features. Inoculations with mixtures of conidia showed that the double mutant strain is slightly less virulent than the wild-type strain. We conclude that Alp and restrictocin are not important virulence determinants in pulmonary infection.     

Purification and crystallization of complexes modeling the active state of the fragile histidine triad protein

Fragile histidine triad protein (Fhit) is a diadenosine triphosphate (ApppA) hydrolase encoded at the human chromosome 3 fragile site which is frequently disrupted in tumors. Reintroduction of FHIT coding sequences to cancer cell lines with FHIT deletions suppressed the ability of these cell lines to form tumors in nude mice even when the reintroduced FHIT gene had been mutated to allow ApppA binding but not hydrolysis. Because this suggested that the tumor suppressor activity of Fhit protein depends on substrate-dependent signaling rather than ApppA catabolism, we prepared two crystalline forms of Fhit protein that are expected to model its biologically active, substrate-bound state. Wild-type and the His96Asn forms of Fhit were overexpressed in Escherichia coli, purified to homogeneity and crystallized in the presence and absence of ApppA and an ApppA analog. Single crystals obtained by vapor diffusion against ammonium sulfate diffracted X-rays to beyond 2.75 A resolution. High quality native synchrotron X-ray data were collected for an orthorhombic and a hexagonal crystal form.      

Pentalithium Ferrite (Li5FeO4) as Highly Active Material for Hydrogen Production in the Chemical Looping Partial Oxidation of Methane

In this work, the pentalithium ferrite was synthetized by solid-state method, and it was characterized by XRD and N₂ adsorption–desorption techniques. Then, H₂ production was obtained through a catalytic conversion process; chemical looping partial oxidation (CLPO) of methane using Li₅FeO₄ as multifunctional material. The catalytic decomposition of methane is an easy way to obtain clean energy, such as hydrogen, but in this process carbon deposition is also possible. The results showed that this material has the ability to convert methane to hydrogen, but it is also capable to donate oxygen atoms from its crystalline network, producing carbon monoxide and/or carbon dioxide, limiting the carbon deposition on the ceramic surface. In addition, it was demonstrated that this lithium-based ceramic produces hydrogen over a wide temperature range (550–900 °C), with a stable hydrogen production for 3 h at 825 °C. Furthermore, it was possible to achieve a cyclic test of methane decomposition with a pre-oxidation process between each cycle obtaining an outstanding increase in hydrogen production from 10% in cycle 1 to ~?100% in the cycle 10. This previous stage not only induces an increase in the decomposition of methane, but also avoids carbon deposition accompanied by the production of both CO_X compounds. Finally, it must be mentioned that Li₅FeO₄ is capable to chemisorb both carbon oxides produced, promoting high purity hydrogen production.

     

CO<Subscript>2</Subscript> adsorption at high pressures in MCM-41 and derived alkali-containing samples: the role of the textural properties and chemical affinity

The adsorption properties of N₂ and CO₂ of MCM-41 and derived alkali-containing samples were analyzed over a wide range of pressures (up to ~4500 kPa) and temperatures (between 30 and 300 °C). The high-pressure and high-temperature experiments were carried out on pure MCM-41 and K- and Na-impregnated derived samples. It was analyzed the influence of pressure and temperature on the CO₂ capture capacity on pure and impregnated samples. The adsorption performance was correlated to the structure and textural properties of the materials using X-ray diffraction and N₂ adsorption–desorption measurements. The addition of an alkaline element changes the textural properties of the material increasing the pore size, which positively affected the CO₂ adsorption capacity of these materials at high pressure. In addition, the isosteric heats of adsorption gave information about the chemical affinity between the impregnated materials and CO₂. The CO₂ adsorption at ~ 4500 kPa for the samples with 5 wt% Na at 100 and 200 °C were 77.98 and 9.79 mmol g^?1, respectively, while the pure MCM-41 adsorbs only 8.92 mmol g^?1.     

有限精度时间自动机的可达性检测

为了缓解状态空间爆炸问题,减小模型检测过程中生成的状态空间,加快模型检测速度,引入有限精度时间自动机(finite precision timed automata,简称FPTA)作为实时系统的形式模型,并提出了一种数据结构SDS(series of delay sequence)符号化表示状态空间中的状态集.FPTA只记录时钟变量的整数值及时钟变化的先后次序,从而减小生成的状态空间.在一定的时间约束下,Alur与Dill提出的时间自动机的可达性检测可简化为FPTA的可达性检测.举例描述了状态空间的生成过程和表示方法.最后,列出部分初步的实验结果,分析了SDS的特点及不足.     

Proteomics in ocular fluids

The focus of this article is to review recent techniques in proteomic analysis of ocular fluids. These fluids include tears, aqueous humor, and vitreous, they will also be compared to serum analysis. Furthermore, we attempt to summarize some disease correlated biomarkers in ocular fluids that were discovered through different proteomic techniques in eye diseases like dry eye, glaucoma, age-related macular degeneration, uveitis, or diabetic retinopathy. This review is trying to point out the importance of these biomarkers for clinical applications.     

A comparison of optical and nuclear methods for the measurement of the thickness of thin metal foils

The areal mass densities and the geometrical thicknesses of non-supported vacuum-deposited metal foils are derived from the results of three differen experiments. Rutherford backscattering, energy loss measurements and the Tolansky technique were applied to identical samples. Systematic discrepancies were found; these are explained in terms of contaminations and roughness of the surfaces.     

A URI-based approach for addressing fragments of media resources on the Web

To make media resources a prime citizen on the Web, we have to go beyond simply replicating digital media files. The Web is based on hyperlinks between Web resources, and that includes hyperlinking out of resources (e.g., from a word or an image within a Web page) as well as hyperlinking into resources (e.g., fragment URIs into Web pages). To turn video and audio into hypervideo and hyperaudio, we need to enable hyperlinking into and out of them. The W3C Media Fragments Working Group is taking on the challenge to further embrace W3C??s mission to lead the World Wide Web to its full potential by developing a Media Fragment protocol and guidelines that ensure the long-term growth of the Web. The major contribution of this paper is the introduction of Media Fragments as a media-format independent, standard means of addressing media resources using URIs. Moreover, we explain how the HTTP protocol can be used and extended to serve Media Fragments and what the impact is for current Web-enabled media formats.     

Ada图形用户界面的实现策略

在UNIX环境下实现Ada的图形用户界面一直是一个比较困难的问题。X/Motif Ada Bindings是一个Ada与Motif工具箱的低级接口工具集,它可用于实现Ada的图形用户界面,本文从它的解决方案、体系结构及接口机制等几个方面对此软件包的实现进行了比较深入的研究分析,并基于此软件包和现有操作平台提供了一种高效的Ada GUI实现策略。