Analysis of three restriction fragment length polymorphisms in the human type II procollagen gene

Cloned genomic DNA sequences corresponding to various regions of the human type II procollagen gene were used to analyze the DNA from 78 normal volunteers. Southern hybridization experiments detected polymorphic HindIII, BamHI, and EcoRI sites. The presence of the polymorphic HindIII site results in a 7.0-kilobase (kb) band, and the absence of this site results in a 14.0-kb band. When present, the BamH1 polymorphic site yields a 4.8-kb band, and when absent, yields a 7.2-kb band. The presence of the EcoRI polymorphic site results in a 3.7-kb band, and its absence results in a 7.0-kb band. Each polymorphic site was mapped. Analyses of the data demonstrated that the sites are present in overall gene frequencies of .39 for HindIII, .04 for BamHI, and .02 for EcoRI. Gene frequencies of the polymorphic sites were also studied with respect to race. The polymorphic sites are present in a Hardy-Weinberg distribution in the study population. Study of an extended family demonstrated that the segregation of the HindIII polymorphic site is consistent with Mendelian inheritance.     

Reception of Nonhomogeneous Signal Fields in the Presence of Homogeneous Noise Fields

The reception of a nonhomogeneous signal field embedded in a homogeneous noise field, using a simple two-element subtractive array, is studied. For element spacing which is small compared to both the distance to the source and to the center-frequency-wavelength, the signal-to-noise improvement over a single element is shown to be independent of element spacing d, relatively independent of bandwidth and the noise spatial distribution, and to depend only on the distance to the signal source in wavelengths and on the signal ground-wave attenuation rate.     

Mixed mode stress intensity distributions for part circular surface flaws

Using the frozen stress method, mixed mode stress intensity factor (SIF) distributions were obtained for a series of low aspect ratio part circular surface flaws in flat plates over a depth to plate thickness range of 0.20–0.70. Mode I results were compared with Mode I solutions in order to sense the influence of the crack surface inclination to the free boundary. It was found that maximum SIF values did not necessarily occur at maximum flaw depth. A rationale is offered to explain this observation. It was also found that the effect of the boundary inclination was to reverse the relative magnitudes of $\text{K}{}_1\text{K}\text{?}{}_1$ and $\text{K}{}_2\text{K}\text{?}{}_2$ in going from a 30° to a 60° crack surface inclination with the boundary.     

The effect of wastewater phosphorus removal on shagawa lake, Minnesota: phosphorus supplies, lake phosphorus and chlorophyll a

In early 1973, the phosphorus supply to Shagawa Lake, Minnesota, was reduced by about 80% when a tertiary wastewater treatment plant began operating. Significant reductions in total and soluble reactive phosphorus concentrations have occurred in the lake since that time. By 1976 the average (volume weighted over the entire lake) total and soluble reactive phosphorus concentrations had declined from about 51 and 21 μg l⁻¹ to about 30 and 4.5 μg l⁻¹, respectively, corresponding to 40 and 80% reductions. During 1975 and 1976, chlorophyll a (averaged over the top 5 m) had decreased to less than 50% of the pretreatment level during May-June but during July-August little change had occurred.

A phosphorus residence time model projected equilibrium total phosphorus concentrations of about 12 μg l⁻¹ within 1.5 years. The fact that this level was not reached is attributed to a feedback of phosphorus from the sediments, primarily during summer. This phenomenon has been incorporated into a modified total phosphorus mass balance model which projects the phosphorus pattern through 1976 quite accurately. The close fit of the modified mass balance model implies that the phosphorus supply from the sediments has not diminished since treatment since treatment began, and that further recovery of the lake will depend upon how long this feedback of phosphorus from the sediments continues.