Middle cerebral artery microneurosurgical embolectomy

Cerebral embolism carries significant mortality and morbidity rates. Middle cerebral artery embolectomies which have been carried out in man with variable results, have been reported by different authors, In order to define a time limit for embolectomies before irreversible damage has been incurred, an experimental embolus model was used in dogs. It was observed that embolectomy carried out at 2, 3, and up to 5 hours after embolism was relatively safe and effective. Beyond 5 hours, embolectomy carried an increased risk of death and morbidity.     

Measuring attendance: A reply to Ilgen.

D. R. Ilgen's (1977) objections to the use of attendance as a performance criterion are discussed in terms of R. L. Thorndike's (1949) criteria for criteria, namely, relevance, reliability, freedom from contamination, and practicality. The argument is made that trying to interpret correlations involving an absenteeism measure that has little or no stability is relatively futile, regardless of whether other estimates of reliability (e.g., interobserver) are high. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Mucocutaneous lymph node syndrome

Mucocutaneous lymph node syndrome represents a series of clinical findings that has been observed primarily in Japanese children. The disease now appears to be migrating to this country. It involves the cervical lymph nodes, the skin, and mucus membranes. Although the course is usually benign and self-limiting, a number of deaths have resulted from coronary artery disease.     

Standardized management of patients and employees exposed to pertussis

Waning pertussis immunity and spread of pertussis by asymptomatic adults contributes to increased pertussis exposures of vulnerable children. The Santa Clara Valley Medical Center had 49 pertussis exposures between July 1, 1989, and June 30, 1997, which originated in pediatric units or clinics and had an impact on the Employee Health Service (EHS) and Emergency Department (ED). We have developed a standardized protocol for management of employees and patients exposed to pertussis. The protocol includes a checklist for infection control staff; memoranda to exposed units conveying exposure information, instructions for employees to report to EHS or ED, and disease symptom information; written guidelines for physician management of patient exposures (prophylaxis and isolation) and EHS or ED management of employee exposures; and prophylaxis recommendations. We allow exposed employees to work while wearing a mask (worn until 5 days of prophylaxis are completed or for the entire potential contagious period if prophylaxis was refused). Employees who develop pertussis are restricted from work. Our protocol and standardized forms provide consistent management of pertussis exposures in both patients and employees.     

Relationship of client abuse with locus of control and gender: A longitudinal study in mental retardation facilities.

Direct-care workers (158 women and 154 men) participated in a study of aggressive work behavior. Employees completed J. B. Rotter's (1966) Locus of Control Scale. Client abuse data were collected over a 2-yr period. Individuals reporting higher levels of external locus of control and men were more likely to emit aggressive behavior than were people reporting lower levels of external locus of control and women. Results supported the hypotheses and suggested that individual and group differences may be useful in understanding maladaptive, aggressive work behavior. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Analysis of FHIT transcripts in cervical and endometrial cancers

We show that mammalian cells can be stably transfected by a mechanical loading procedure in which cells are forced through a small opening in the presence of DNA. A suspension of cells and plasmid DNA in growth medium was passed up and down through a 30-gauge needle attached to a 1-ml syringe. Cells were immediately plated at appropriate densities for subsequent selection for stable expression of a marker gene. Two rodent cell lines, Chinese hamster ovary and mouse Ltk- cells, were successfully transfected with an efficiency of about one transfectant per 5 x 10(4) cells. The human HeLa cell line was transfected with a somewhat lower efficiency. Pluronic F-68, a detergent believed to aid in healing of membrane injuries, had no beneficial effect when present during the loading procedure. Successful transfection was accomplished using three different genes as selectable markers. Southern blotting analysis revealed that transfectants contained one or very few copies of the introduced DNA construct integrated into the genome. Several transfectants were demonstrated to remain stable for more than 20 generations of growth in the absence of selection. This procedure is fast, economical, and of general utility.     

The in vivo effect of colchicine on the addition of galactose and sialic acid to rat hepatic serum glycoproteins

Colchicine inhibits the secretion of plasma protein by rat hepatocytes and causes their intracellular accumulation in Golgi-derived secretory vesicles. This study examines whether colchicine affects secretion before or after galactose and sialic acid have been added to the secretory glycoproteins. D-[G-3H] Galactose was injected into rats and was found to be incorporated into serum glycoproteins contained within Golgi-derived secretory vesicles. The administration of colchicine (25 mumol/100 g, body weight), immediately before the injection of D-[G-3H] galactose, caused an increase in radioactivity of the serum glycoproteins in these cell fractions. D-[G-3H] Glucosamine was incorporated into serum glycoproteins contained within the rough and smooth endoplasmic reticulum and the Golgi cell fractions; however, its incorporation into the sialic acid moieties of these proteins only occurred in Golgi-derived cell fractions. Colchicine administration resulted in an increased incorporation of D-[G-3H] glucosamine into the sialic acid residues of serum glycoproteins contained within the Golgi cell fractions. These data indicate that colchicine inhibits secretion of serum proteins by rat liver after the addition of galactose and sialic acid to the secretory proteins has taken place.