全文获取类型
收费全文 | 1268篇 |
免费 | 1篇 |
专业分类
电工技术 | 1篇 |
化学工业 | 13篇 |
建筑科学 | 2篇 |
轻工业 | 1篇 |
无线电 | 5篇 |
一般工业技术 | 8篇 |
冶金工业 | 1236篇 |
自动化技术 | 3篇 |
出版年
2019年 | 2篇 |
2015年 | 1篇 |
2012年 | 1篇 |
2011年 | 1篇 |
2009年 | 1篇 |
2006年 | 2篇 |
2005年 | 2篇 |
2004年 | 2篇 |
2003年 | 2篇 |
2002年 | 2篇 |
2001年 | 1篇 |
2000年 | 3篇 |
1999年 | 45篇 |
1998年 | 399篇 |
1997年 | 222篇 |
1996年 | 149篇 |
1995年 | 72篇 |
1994年 | 65篇 |
1993年 | 69篇 |
1992年 | 12篇 |
1991年 | 12篇 |
1990年 | 16篇 |
1989年 | 20篇 |
1988年 | 25篇 |
1987年 | 17篇 |
1986年 | 9篇 |
1985年 | 16篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1982年 | 3篇 |
1981年 | 6篇 |
1980年 | 7篇 |
1978年 | 3篇 |
1977年 | 16篇 |
1976年 | 62篇 |
1975年 | 1篇 |
1955年 | 1篇 |
排序方式: 共有1269条查询结果,搜索用时 15 毫秒
101.
p53 antibodies in patients with various types of cancer: assay, identification, and characterization 总被引:2,自引:0,他引:2
R Lubin B Schlichtholz JL Teillaud E Garay A Bussel CP Wild 《Canadian Metallurgical Quarterly》1995,1(12):1463-1469
Alteration of the p53 gene is the most frequent genetic alteration in human cancer and leads to the accumulation of mutant p53 in the nucleus of tumor cells. In addition, it has been shown that patients with various types of neoplasia have p53 antibodies in their sera which could be used as an indirect diagnostic procedure for p53 alteration. Using a new ELISA, we have analyzed the sera from more than 1000 patients with various types of cancer and from healthy blood donors. We demonstrate that p53 antibodies are detected mainly in cancer patients and are strictly proportional to the occurrence of p53 mutations. Using various immunological approaches, these antibodies were unambiguously demonstrated to be directed toward the human p53 protein. Isotyping analysis of these antibodies strongly suggested that they correspond to a humoral response to the p53 protein which accumulates in the tumor cell. This finding suggests that serological analysis, combined with histochemistry, is suitable for assessing the integrity of the p53 gene in cancer patients. 相似文献
102.
KR Edge CP McClarty SJ Krige RR Rantalainan 《Canadian Metallurgical Quarterly》1995,33(3):117-9; discussion 119-20
Four hundred and eight consecutive patients scheduled for cataract surgery under local anaesthesia were randomly allocated to one of two types of regional anaesthesia. One group was given the standard retrobulbar block combined with a facial nerve block of the O'Brien type, and the second group was given a modified peribulbar block. Results indicate that it is possible to achieve a high degree of reliability, within a relatively short time, from the modified peribulbar block without the major complications associated with retrobulbar block. 相似文献
103.
JO Bordin JG Kelton MN Warner JW Smith GA Denomme TE Warkentin K McGrath R Minchinton CP Hayward 《Canadian Metallurgical Quarterly》1997,37(8):823-828
BACKGROUND: Immunization to platelet alloantigens can occur during pregnancy or after the transfusion of blood components. Platelet alloantibodies can cause neonatal alloimmune thrombocytopenia and posttransfusion purpura. Transfusion-induced alloimmunization to a novel platelet alloantigen system, Gov, expressed on the 175-kDa glycosyl phosphatidylinositol-anchored platelet glycoprotein, CD109, was previously described. This report describes three unrelated patients who were alloimmunized to Gov(a) or Gov(b) during pregnancy. STUDY DESIGN AND METHODS: Platelets were typed by using radioimmunoprecipitation for HPA-1a, -3a, -5a, -5b, Gov(a), and Gov(b) and by polymerase chain reaction-restriction fragment length polymorphism for HPA-1a, -1b, -3a, and -3b. Maternal sera were screened for platelet antibodies by using radioimmunoprecipitation and the antigen capture assay. RESULTS: Patients 1 and 2 were investigated after the diagnosis of neonatal alloimmune thrombocytopenia in their children, and alloantibodies specific for Gov(b) and Gov(a), respectively, were detected in maternal serum. Serum from patient 3, who had mild idiopathic thrombocytopenia purpura with no detectable autoantibody, was found to contain alloantibodies to Gov(b) and to HPA-5b, presumably as a result of immunization during pregnancy. Platelet typings confirmed that the patients were at risk for alloimmunization to the respective antigen. CONCLUSION: This report of three cases of maternal alloimmunization to antigens in the Gov system indicates that immunization can occur via placental transfer of antigen and that Gov system alloantibodies may be associated with neonatal alloimmune thrombocytopenia. 相似文献
104.
105.
106.
107.
108.
109.
DG Cable T O''Brien IJ Kullo RS Schwartz HV Schaff VJ Pompili 《Canadian Metallurgical Quarterly》1997,35(3):553-559
PURPOSE: To evaluate the influence of prognostic factors in postoperative radiotherapy of NSCLC with special emphasis on the time interval between surgery and start of radiotherapy. METHODS AND MATERIALS: Between January 1976 and December 1993, 340 cases were treated and retrospectively analyzed meeting the following criteria: complete follow-up; complete staging information including pathological confirmation of resection status; maximum interval between surgery (SX) and radiotherapy (RT) of 12 weeks (median 36 days, range 18 to 84 days); minimum dose of 50 Gy (R0), and maximum dose of 70 Gy (R2). Two hundred thirty patients (68%) had N2 disease; 228 patients were completely resected (R0). One hundred six (31%) had adenocarcinoma, 172 (51%) squamous cell carcinoma. RESULTS: In univariate analysis, Karnofsky performance status (90+ >60-80%; p = 0.019 log rank), resection status stratified for nodal disease (R+ 相似文献
110.
Michele Miranda M Lúcia CP da Silva Heizir F de Castro 《Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986)》2006,81(4):566-572
The aim of this work was to establish appropriate conditions for immobilising Candida rugosa lipase on a low‐cost inorganic matrix, hydrous niobium oxide, using a multivariate statistical approach. A 23 full factorial design was employed to determine the effects of support activation with glutaraldehyde (concentration 2.5–4.5%, pH 7–10) and lipase loading (200‐700 U g?1 matrix) on the hydrolytic and synthetic activities of the immobilised derivatives. From the results the following conditions were established: lipase loading of 450 U g?1 matrix and niobium oxide activation with glutaraldehyde at a concentration of 2.5% and pH 8. Under these conditions, high activity recovery (47.21%) and esterification yield (86.90%) were attained. The results also show that hydrous niobium oxide can be a valid alternative to replace high‐cost, commercially available inorganic matrices such as controlled pore silica. Copyright © 2006 Society of Chemical Industry 相似文献