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91.
N Deshmukh J Scotson AR Dodson PH Smith Y Ke CS Foster 《Canadian Metallurgical Quarterly》1997,80(6):869-874
OBJECTIVES: Transitional cell carcinomas of upper urinary tract (uttTCC) constitute 5% to 6% of all urothelial tumors. Ureteropyeloscopy has become the standard for clinical evaluation of uutTCC. Moreover, endoscopic treatments have been advocated as a conservative approach for low grade tumors or patients with intermediate grade tumors whose renal function is compromised. Therefore, grading has become the most predictive variable in defining therapeutic approach. In addition to morphologic evaluation, a series of biologic markers may be used to increase the accuracy of grading such as DNA analysis and p53 protein expression. In this study, we have evaluated these markers by means of cell image analysis with the SAMBA 400 system. METHODS: Thirteen cases of uttTCC were studied with cytologic smear, cell block, and histologic confirmation. DNA analysis was performed on cytologic smear. Immunostaining was performed on cell blocks. A grade was assigned on the basis of DNA evaluation and p53 expression quantitation. These grades were combined for each case and compared with the initial cytologic grading and the final histologic grading. RESULTS: Cytology alone diagnosed TCC in all but 1 case that was diagnosed atypical. Discrepancies were found in primary grading: cytologic grading concurred with histologic grading in 6 of the 13 cases. CONCLUSIONS: These results, although in a limited but selected number of cases, show the potential of computerized evaluation of biologic markers as parameters for a more objective grading of tumors. 相似文献
92.
CW Taylor LM Wang AF List D Fernandes GD Paine-Murrieta CS Johnson RL Capizzi 《Canadian Metallurgical Quarterly》1997,33(10):1693-1698
The objectives of this study were to evaluate the protective effects of amifostine against paclitaxel-induced toxicity to normal and malignant human tissues. Haematopoietic progenitor colony assays were used to establish the number of CFU-GEMM and BFU-E colonies after incubation with WR-1065 alone, Amifostine alone, paclitaxel (2.5 or 5 microM) +/- WR-1065 or amifostine. MTT and alkaline elution assays evaluated the in vitro growth inhibitory and DNA damaging effects, respectively, of paclitaxel with or without amifostine against normal human fibroblasts and human non-small cell lung cancer (NSCLC) cells. This combination was also evaluated in vivo using severe combined immune deficient (scid) mouse models of early (non-palpable tumours) and advanced (palpable tumours) human ovarian cancer. Human 2780 ovarian cancer cells were inoculated subcutaneously while paclitaxel and amifostine were administered intraperitoneally. A brief exposure (15 min) to amifostine not only protected human haematopoietic progenitor colonies from paclitaxel toxicity, but stimulated the growth of CFU-GEMM and BFU-E beyond control values. Amifostine protected normal human lung fibroblasts from paclitaxel-induced cytotoxicity and DNA single-strand breaks. However, paclitaxel cytotoxicity and DNA single-strand breaks were actually enhanced by pretreatment with amifostine in the NSCLC model. Importantly, amifostine did not interfere with paclitaxel antitumour activity even with prolonged exposure (24.5 h) of the lung cancer cells to high concentrations (1.2 mM) in vitro or following five repetitive high doses (200 mg/kg) given to scid mice with human ovarian cancer xenografts. Indeed, under certain circumstances, amifostine resulted in sensitisation of tumour cells to paclitaxel. Our results confirm previous reports of the ability of amifostine to protect normal tissues from the toxic effects of chemotherapy drugs and now extend these observations to paclitaxel. 相似文献
93.
MF Chen YY Jan CS Wang TL Hwang LB Jeng SC Chen TC Chao 《Canadian Metallurgical Quarterly》1997,84(9):1229-1232
The effect of phenylephrine-induced reflex parasympathetic stimulation on QT interval and its dispersion was studied in 16 healthy subjects with a history of paroxysmal supraventricular tachycardia, both during sinus rhythm and during atrial pacing. Results demonstrate that rapid reflex parasympathetic stimulation does not influence QT interval duration or QT dispersion, and also emphasize the inappropriateness of Bazett's formula, the need for comparison of QT intervals during identical heart rates, and the importance of analyzing all 12 leads of a standard electrocardiogram when assessing the effects of various interventions on the QT interval. 相似文献
94.
Miros?aw Marek Kasprzak Helle Nygaard L?rke Knud Erik Bach Knudsen 《International journal of molecular sciences》2012,13(12):16833-16852
Different extraction, purification and digestion methods were used to investigate the molecular properties of carbohydrates in arabinoxylan and β-glucan concentrates, dietary fiber (DF) rich breads and ileum content of bread fed pigs. The breads studied were: a low DF wheat bread (WF), whole meal rye bread (GR), rye bread with kernels (RK), wheat bread supplemented with wheat arabinoxylan concentrate (AX) and wheat bread supplemented with oat β-glucan concentrate (BG). The weight average molecular weight (Mw) of extractable carbohydrates in β-glucan concentrate decreased eight-fold after inclusion in the BG bread when exposed to in vitro digestion, while the Mw of purified extractable carbohydrates in AX bread was reduced two-fold, and remained almost unaffected until reaching the terminal ileum of pigs. Similarly, the Mw of purified extractable carbohydrates in GR and RK bread was not significantly changed in the ileum. The AX bread resulted in the highest concentration of dissolved arabinoxylan in the ileum among all the breads that caused a substantial increased in ileal AX viscosity. Nevertheless, for none of the breads, the Mw of extractable carbohydrates was related neither to the bread extract nor ileal viscosity. 相似文献
95.
Confocal Raman microscopy (CRM) has been used to extract dye diffusion data from poly(ethylene terephthalate) (PET) fibres dyed with Disperse Yellow 23 under supercritical CO2 conditions. Spectral information as a function of depth was measured in a non-destructive manner using dry and oil objectives in confocal Raman mode. Mapping along the radius of the fibre cross-section was performed and compared to the data from the confocal measurements. A significant dye concentration gradient has been observed along the line normal to the fibre surface which the data from the oil confocal measurements accurately describes to a depth of 45 μm. The effect of the supercritical CO2 dyeing process on the fibre morphology has also been evaluated using CRM. 相似文献
96.
Synthesis of Tribologically Favorable Coatings for Hot Extrusion Tools by Suspension Plasma Spraying
M. Erne D. Kolar C. Hübsch M. M?hwald Fr.-W. Bach 《Journal of Thermal Spray Technology》2012,21(3-4):668-675
Up to now, no coating systems have been marketed in the field of direct hot extrusion, which provide both surface protection of the parts in contact with the billet (i.e., container and die) as well as a significant reduction of the frictional losses induced by the billet passing over the container walls. To dispense with the use of lubricants and to enhance the usable forming capacity and therefore the efficiency of the process, different oxide ceramics were prepared in one suspension and plasma sprayed to produce coatings. The aim was to reach a sufficient level of feedstock mixing to obtain deterministic solid solutions of the oxide phases in coatings resulting in a reduction of their coefficient of friction under dry sliding conditions. To achieve this objective, the high specific surface area of nanosized feedstock with primary particle sizes below 100?nm was used. By means of x-ray diffraction it could be proven, that the desired phases could be synthesized to varying ratios regarding the different coating systems considered here. Besides the experimental work, the fundamentals of the mixing process of different oxides are discussed with regard to the crystallographic aspects. 相似文献
97.
Christian Klose Gregor Mroz Gian Luigi Angrisani Kai Kerber Wilfried Reimche Friedrich-Wilhelm Bach 《Production Engineering》2013,7(1):35-41
Magnetic magnesium alloys can be utilized as a load sensitive material, in which the inverse magnetostrictive effect is used in order to measure the actual loads in structural components manufactured from such lightweight sensor alloys. To achieve a material which exhibits magnetic properties, Mg is alloyed with ferromagnetic materials like cobalt or samarium-cobalt. Alloying elements commonly used with Mg are utilized to improve the mechanical properties of these alloys, which however may have a slight negative impact on the magnetic sensitivity. In this work, two separate magnetic Mg alloys are compared, each with properties matched to the opposing requirements: (a) high load sensitivity and (b) satisfactory mechanical properties, respectively. The precipitation behavior of the ferromagnetic constituent Co in Mg together with other alloying elements is shown on the basis of SEM images. In addition, the dissolving behavior of the Co powder during the casting process of a binary Mg–Co alloy is investigated. Cyclic loading tests employing harmonic analyses of eddy current signals are utilized in order to verify the alloys’ sensory properties. The mechanical properties are investigated using tensile tests. 相似文献
98.
Anh Tuan Thanh Pham Oanh Kieu Truong Le Dung Van Hoang Truong Huu Nguyen Trang Huyen Cao Pham Phuong Thanh Ngoc Vo Thang Bach Phan Vinh Cao Tran 《Journal of the European Ceramic Society》2021,41(6):3493-3500
Structure modification has been found to tune significantly the transparent-conducting performance, especially mobility and conductivity of hydrogenated Ga-doped ZnO (HGZO) films. The strong correlation between film thickness and mobility of the films is revealed. The mobility increases quickly with increasing the thickness from 350 to 900 nm, and then tends to be saturated at further thicknesses. A higher mobility than 50 cm2/Vs can be achieved, which is an extra-high value for polycrystalline ZnO films deposited by using the sputtering technique. The thickness-dependent mobility originates from scatterings on grain boundaries and dislocation-induced defects controlled by thin-film growth. Based on the Volmer-Weber model, an expansion model is built up to describe the thickness-dependent crystal growth of the HGZO films, especially at the thick films. As a result, the 800 nm-thick HGZO film obtains the highest performance with high mobility of 51.5 cm2/Vs, low resistivity of 5.3 × 10?4 Ωcm, and good transmittance of 83.3 %. 相似文献
99.
Niu YD Xu Y McAllister TA Rozema EA Stephens TP Bach SJ Johnson RP Stanford K 《Journal of food protection》2008,71(4):691-698
This study was conducted to compare fecal grab (FEC) and rectoanal mucosal swab (RAMS) techniques as sampling methods for surveillance of Escherichia coli O157:H7 in conjunction with administration of a mitigation therapy. The study was nested within a larger experiment that investigated bacteriophage as a preharvest strategy for controlling E. coli O157:H7 in feedlot steers. Samples (FEC and RAMS) were collected from 16 of the 32 feedlot steers (control and oral bacteriophage treatment; n = 8) involved in the mitigation study. All steers had been inoculated on day 0 with 10(10) CFU of nalidixic acid-resistant E. coli O157:H7, and samples were collected on 16 occasions over the next 83 days. FEC samples were assessed by direct plating of serial dilutions in PBS, plus a 6-h enrichment and immunomagnetic separation when E. coli O157:H7 concentrations were below limits detectable by direct plating (i.e., <1 log CFU/g). All RAMS samples were assessed by enrichment and immunomagnetic separation. E. coli O157:H7 was detected more frequently (P < 0.01) by FEC than by RAMS. Overall, 213 of 256 samples were positive either by FEC or RAMS. Discrepancies between sampling techniques were observed in 63 of the 213 positive samples; FEC missed 11 samples that were positive by RAMS, and RAMS missed 52 of those positive by FEC (miss rates of 5.16 and 24.41%, respectively). Kappa values (0.36 to 0.45) indicated only fair to moderate agreement between FEC and RAMS results, but this agreement was higher at lower levels of E. coli O157:H7 shedding (later in the experimental period). Selection of sampling procedure could significantly influence the assessed merit during testing of potential strategies for controlling E. coli O157:H7 on the farm. 相似文献
100.
Hanne Boskov Hansen Claus V Rasmussen Knud Erik Bach Knudsen se Hansen 《Journal of the science of food and agriculture》2003,83(1):76-85
The aim of this study was to measure the variation in dietary fibre (DF) content and composition among different rye varieties grown in the same location in three successive years and to estimate the contributions of genotype and harvest year to this variation. The study included grain from 19 different varieties of rye, of which seven varieties were analysed in all three years. The content of total DF in all samples ranged from 147 to 209 g kg?1 dry matter (dm), with 34–66 g kg?1 being water‐extractable (WE) DF and 108–159 g kg?1 being water‐unextractable (WUE) DF. The main DF component was arabinoxylan (AX) with a content of 80–121 g kg?1 dm, of which 26–41 g kg?1 dm was WE AX. The ratio of arabinose to xylose (ara/xyl) of total AX ranged from 0.59 to 0.69. The content of β‐glucan was 13–22 g kg?1 dm and that of fructan was 45–64 g kg?1 dm. Analysis of the data from seven varieties and three harvest years showed a highly significant influence of harvest year on most DF components. Significant genotype effects were found for the contents of total DF, total AX, β‐glucan and fructan and for the ara/xyl ratio of total and WUE AX. Estimation of variance components showed that yearly variations in the contents of total DF, total AX, WUE DF, WUE AX and β‐glucan were generally higher (27–55% of total variance) than genotype effects (14–19%). The ara/xyl ratio of total and WUE AX showed a high influence of genotype (46 and 43% respectively). Non‐adapted rye varieties with small kernel size had the highest contents of total DF and total AX. The content of β‐glucan was positively correlated with kernel weight (r = 0.68), whereas no overall correlation was found between kernel size and the content of DF. As a conclusion, the variation in DF content and composition in this study was significantly influenced by both harvest year and rye genotype. © 2002 Society of Chemical Industry 相似文献