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Unionized construction workers in a major midwestern city were surveyed to collect data on education, training, experience, employment history, and a variety of other demographic variables. The characteristics of this local work force were analyzed and, where possible, contrasted with the data on the national construction labor force. The implications of the findings were discussed as they relate to the need for human resource planning in the construction industry. For example, the data indicated that younger workers are not joining unions in the same numbers as in the past. The anticipated result is a future shortage of skilled workers in the unionized sector of the industry, which has implications for construction productivity and for the funding of various worker benefits. The need for improved human resource planning was stressed. 相似文献
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Small inverted repeats (small palindromes) on plasmids have been shown to mediate a recombinational rearrangement event in Escherichia coli leading to the formation of inverted dimers (giant palindromes). This recombinational rearrangement event is efficient and independent of RecA and RecBCD. In this report, we propose a cruciform-dumbbell model to explain the inverted dimer formation mediated by inverted repeats. In this model, the inverted repeats promote the formation of a DNA cruciform which is processed by an endonuclease into a linear DNA with two hairpin loops at its ends. Upon DNA replication, this linear dumbbell-like DNA is then converted to the inverted dimer. In support of this model, linear dumbbell DNA molecules with unidirectional origin of DNA replication (ColE1 ori ) have been constructed and shown to transform E.coli efficiently resulting in the formation of the inverted dimer. The ability of linear dumbbell DNA to transform E.coli suggests that the terminal loops may be important in bypassing the requirement of DNA supercoiling for initiation of replication of the ColE1 ori. 相似文献
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Defining a strain‐induced time constant for oriented low shear‐induced structuring in high consistency MFC/NFC‐filler composite suspensions
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Micro and nanofibrillated cellulose is an essentially one‐dimensional high aspect‐ratio particle material, which can undergo two‐dimensional layer (band) structuring under shear. Controlling the evolving rheological properties in aqueous suspension is essential for industrial applications in composite materials. This study focuses on an as yet considered to be unreported phenomenon of structure hardening under low shear. The timescale of the quasi gelation‐controlled structure formation under low shear is studied using the large gap vane‐in‐cup geometry of the Brookfield viscometer. It is proposed that localized structure forms within continuous shear bands, similar to quasi liquid‐crystal formation. By extrapolating a characteristic structure growth parameter to the rotation speed at which it becomes zero, the strain‐induced structure time constant, tgel, can be obtained as (= f (Ω)) = 1/tgel for the range Ω = 10–100/min. The time constant of low shear structure formation is shown to be separable from the static viscoelastic structure build under oscillation in concentrated composite suspension using plate‐plate geometry, which is manifest by a Weissenberg normal force response on switching to applied shear, when the time constant of structuration tgel is long. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132, 42827. 相似文献