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101.
Automatic particle detection through efficient Hough transforms 总被引:4,自引:0,他引:4
Manual selection of single particles in images acquired using cryo-electron microscopy (cryoEM) will become a significant bottleneck when a very large number of images are required to achieve three-dimensional reconstructions at near atomic resolution. Investigation of fast, accurate approaches for automatic particle detection has become one of the current challenges in the cryoEM community. At the same time, the investigation is hampered by the fact that few benchmark particles or image datasets exist in the community. The unavailability of such data makes it difficult to evaluate newly developed algorithms and to leverage expertise from other disciplines. The paper presents our recent contribution to this effort. It also describes our newly developed computational framework for particle detection, through the application of edge detection and a sequence of ordered Hough transforms. Experimental results using keyhole limpet hemocyanin (KLH) as a model particle are very promising. In addition, it introduces a newly established web site, designed to support the investigation of automatic particle detection by providing an annotated image dataset of KLH available to the general scientific community. 相似文献
102.
Despite frequent discussions of what it means to be normal in clinical, social, and personality psychology theory, the characteristics of individuals who call themselves normal are little understood. In 5 studies, the authors investigated various hypotheses concerning the nature of normality evaluations. The authors add to recent evidence that normality evaluations represent a distinct dimension of evaluative judgments, showing self-judgments of being normal (versus strange) to be relatively independent from self-judgments of being average (versus unique). Normality evaluations showed positive relations with communal traits such as agreeableness, conscientiousness, and emotional stability, and were negatively related to openness to experience. Supporting a broader hypothesis that normality evaluations may be involved in directing or motivating personality development processes, normality evaluations were positively associated with well-being and a sense of fitting in with one's peers, and individuals who felt abnormal felt a heightened sense that they needed to improve their personality. Finally, the personality correlates of normality evaluations were found to change over the lifespan, largely in parallel with the actual mean-level development of personality traits with age. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
103.
The results of a study of the variation of three-phase induction machines' input impedance with frequency are proposed. A range of motors were analysed, both two-pole and four-pole, and the magnitude and phase of the input impedance were obtained over a wide frequency range of 20 Hz-1 MHz. For test results that would be useful in the prediction of the performance of induction machines during typical use, a test procedure was developed to represent closely typical three-phase stator coil connections when the induction machine is driven by a three-phase inverter. In addition, tests were performed with the motor's cases both grounded and not grounded. The results of the study show that all induction machines of the type considered exhibit a multiresonant impedance profile, where the input impedance reaches at least one maximum as the input frequency is increased. Furthermore, the test results show that the grounding of the motor's case has a significant effect on the impedance profile. Methods to exploit the input impedance profile of an induction machine to optimise machine and inverter systems are also discussed. 相似文献
104.
Y.V. Gomeniuk A.N. Nazarov Ya.N. Vovk V.S. Lysenko Yi Lu O. Buiu S. Hall R.J. Potter P. Chalker 《Microelectronics Reliability》2007,47(4-5):714
Metal–oxide–semiconductor (MOS) capacitors based on HfO2 gate stacks with Al and TiN gates are compared to study the effect of the gate electrode material to the properties of insulator–semiconductor interface. The structures under study were shown to contain interface trap densities of around 2 × 1011 cm−2 eV−1 for Al gate and up to 5.5 × 1012 cm−2 eV−1 for TiN gate. The peak in the surface state distribution was found at 0.19 eV above the valence band edge for Al electrode. The respective capture cross-section is 6 × 10−17 cm2 at 200 K.The charge injection experiments have revealed the presence of hole traps inside the dielectric layer. The Al-gate structure contains traps with effective capture cross-section of 1 × 10−20 cm2, and there are two types of traps in the TiN-gate structure with cross-sections of 3.5 × 10−19 and 1 × 10−20 cm2. Trap concentration in the structure with Al electrode was considerably lower than in the structure with TiN electrode. 相似文献
105.
I.P. Tyagulskyy I.N. Osiyuk V.S. Lysenko A.N. Nazarov S. Hall O. Buiu Y. Lu R. Potter P. Chalker 《Microelectronics Reliability》2007,47(4-5):726
The paper focuses on the study of charge trapping processes in high-k MOS structures at cryogenic temperatures. It was shown, that there is extremely strong trapping in shallow electron and hole traps, localized in the high-k dielectrics. Concentration of shallow electron traps is as much as 1013 cm−2, while abnormal small capture cross-sections (4.5–8 × 10−24 cm2 for different samples, accordingly) suggests localization of shallow emitting electron traps in transition layer “high-k dielectric/Si”, more, than at the interface. Shallow hole traps with concentration near 1012 cm−2 are separated from silicon valence band with energy barrier in the range 10–39 meV for different samples. 相似文献
106.
Tuneable electrical properties of hafnium aluminate gate dielectrics deposited by metal organic chemical vapour deposition 总被引:1,自引:0,他引:1
Y. Lu O. Buiu S. Hall I.Z. Mitrovic W. Davey R.J. Potter P.R. Chalker 《Microelectronics Reliability》2007,47(4-5):722
Hafnium aluminate (HfAlO) high-k films deposited by Metal Organic Chemical Vapour Deposition (MOCVD) with various Al concentrations were investigated. The results of electrical measurements show the feasibility of adjusting the relative dielectric constant of the layers in a wide range (9–16), when the aluminium concentration varies between 4% and 38%. The minimum leakage current occurs for Al concentrations up to 9%. The thinner films show Poole–Frenkel-like conduction at low field and Fowler–Nordheim-like conduction at moderate/high field, even at higher concentrations of Al into the film, while thicker films show a higher hysteresis due to an increased number of slow trapping centres in the film. 相似文献
107.
BACKGROUND: Dehydroepiandrosterone (DHEA) is among the most abundant steroids in the human body and appears to have diverse biochemical activities. This multifunctional hormone has long been a compound of interest to research psychiatrists. Its recent promotion and availability as an over-the-counter supplement to the general public has led to widespread use. Little is known about potential adverse effects of DHEA when consumed on an acute or chronic basis. We report a case of mania in an older man acutely admitted to our psychiatric facility with no previous personal or family history of bipolar disorder that appeared to be related to recent DHEA use. The patient had initiated DHEA use 6 months prior to admission and was taking 200-300 mg/day at the time of presentation. METHODS: He was treated with valproic acid 500 mg twice daily. RESULTS: The patient showed sufficient improvement to be discharged following a 7-day inpatient hospitalization. CONCLUSIONS: A wide range of medications have been associated with the induction of hypomania and mania, and we have provided a brief discussion of the potential for DHEA to trigger manic symptoms. 相似文献
108.
J A Potter 《Journal of Automated Methods and Management in Chemistry》2001,23(6):189-190
Significant advances within the field of laboratory automation and instrumentation have greatly benefited the pharmaceutical industry in its quest to discover, develop and monitor the quality of its products. Necessitated by the need for efficiency and greater productivity, faster and more cost-effective means of analyses exist in the form of devices made up of complex electromechanical components, all logically controlled and most with the capability to interface with sophisticated information systems. This benefit does come with a price, a greater responsibility to ensure data quality while complying with increased regulatory requirements. Commitment to this responsibility presents a substantial challenge to scientists and managers throughout the industry. Due diligence must be demonstrated. A comprehensive evaluation of every laboratory system utilized, a solid plan of action for correcting any known deficiencies including upgrades or complete replacement, and an accurate monitoring procedure with the ability to measure progress are all absolute necessities to ensure success. Crossfunctional team effott and communication must transpire with full managerial support. Vendors need to be audited, made aware of any functional or quality inadequacies they possess as well as the pharmaceutical industry's expectation for these shortcomings to be rapidly corrected. Suppliers of these systems should also be encouraged to provide complete 'off-the-shelf solutions' to eliminate the need for in-house customization. The requirements for regulatory compliance in today's electronic environment have been well publicized. The players involved are not only listening, but also taking the necessary steps to retain and improve efficiency without sacrificing quality. With the proper measures, planning and action, a highly automated, cost-effective and compliant laboratory operation can become a reality. 相似文献
109.
N Raghunand MI Altbach R van Sluis B Baggett CW Taylor ZM Bhujwalla RJ Gillies 《Canadian Metallurgical Quarterly》1999,57(3):309-312
31p Magnetic resonance spectroscopy (MRS) was employed to investigate tumor pH in xenografts of drug-sensitive and drug-resistant MCF-7 human breast carcinoma cells. Measured extracellular pH values were found to be lower than the intracellular pH in all three tumor types investigated. The magnitude of this acid-outside plasmalemmal pH gradient increased with increasing tumor size in tumors of two drug-resistant variants of MCF-7 cells, but not in tumors of the parent (drug-sensitive) cells. The partitioning of weak-base or weak-acid drug molecules across the plasma membrane of a tumor cell is dependent upon the acid-dissociation constant (pKa) of the drug as well as the plasmalemmal pH gradient. A large acid-outside pH gradient, such as those seen in MCF-7 xenografts, can exert a protective effect on the cell from weak-base drugs such as anthracyclines and Vinca alkaloids, which have pKa values of 7.5 to 9.5. The possibility of enhancing the therapeutic efficacy of weak-base drugs by dietary or metabolic manipulation of the extracellular pH, in order to reduce or reverse the plasmalemmal pH gradient, deserves investigation. 相似文献
110.
K Papadakis EA Chen FI Luks MS Lessin CW Wesselhoeft FG DeLuca 《Canadian Metallurgical Quarterly》1999,17(1):67-69
A triplet pregnancy in a 23-year-old woman was terminated at 15 weeks of gestation because of her severe hypertension, lung edema, and secondary hyperthyroidism. The pregnancy consisted of a hydatidiform mole with a 46,XY karyotype and two fetuses each with 46,XX and a 46,XY karyotype. To determine the zygosity and genetic origin of the mole and fetuses, PCR- and computer-assisted genotyping were performed at 27 CA-repeat marker loci that were distributed evenly over the genome. As a result, genotypes of the three pregnancy products were distinct from each other, indicating that the triplets were trizygotic. The mole lacked any maternal alleles but inherited both of the paternal alleles and/or one paternal allele in duplicate. This, along with the XY sex chromosome constitution, indicated that the mole resulted from dispermic androgenesis. The mother developed a persistent trophoblastic tumor thereafter. 相似文献