Effects of selective excitotoxic lesions of the nucleus accumbens core, anterior cingulate cortex, and central nucleus of the amygdala on autoshaping performance in rats.

The nucleus accumbens core (AcbC), anterior cingulate cortex (ACC), and central nucleus of the amygdala (CeA) are required for normal acquisition of tasks based on stimulus-reward associations. However, it is not known whether they are involved purely in the learning process or are required for behavioral expression of a learned response. Rats were trained preoperatively on a Pavlovian autoshaping task in which pairing a visual conditioned stimulus (CS+) with food causes subjects to approach the CS+ while not approaching an impaired stimulus (CS-). Subjects then received lesions of the AcbC, ACC, or CeA before being retested. AcbC lesions severely impaired performance; lesioned subjects approached the CS + significantly less often than controls, failing to discriminate between the CS + and CS-. ACC lesions also impaired performance but did not abolish discrimination entirely. CeA lesions had no effect on performance. Thus, the CeA is required for learning, but not expression, of a conditioned approach response, implying that it makes a specific contribution to the learning of stimulus-reward associations. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Comparison of the infusion of acetylcholine into the artery of the sinoatrial node with the electric stimulation of cardiac parasympathetic nerves

Atrial fibrillation occurring after open heart surgery largely depends on heterogeneous dispersion of refractoriness. To investigate the contribution of the autonomic nervous system in this phenomenon, we studied the regional distribution of neurally induced atrial electrophysiological events. Electrical stimulation of the right atrial fat pad, acetylcholine injection into the sinus node artery, and stimulation of the right and left vagosympathetic trunks were compared with respect to detailed atrial mapping. Unipolar electrograms were recorded from 127 atrial sites before and after neural stimulation or acetylcholine injection (10(-7) mol) in 8 anesthetized dogs. Regional changes in atrial repolarization were estimated by epicardial isointegral maps generated from computed values of the area under each electrogram and plotted on an atrial grid. The anatomical distribution of the sinus node artery was assessed by intra-arterial injection of microspheres. The effects of right and left vagal and right atrial fat pad stimulation extended contralaterally. Acetylcholine injected into the sinus node artery affected the lower left atrium whereas no microspheres could be found in this region upon microscopic examination. Therefore, this effect was possibly related to cholinergic activation of neuronal cell bodies located in the right atrial wall and projecting to the lower left atrium, supporting the hypothesis that local circuit neurons were involved in the activation of the intrinsic nervous system of the heart.     

Optimizing stochastic gradient descent algorithms for serially addressed adaptive-optics wavefront modulators

High-resolution adaptive-optical systems with thousands to millions of pixels will most likely have to employ serial- or matrix-addressed spatial light modulators (e.g., microelectromechanical-system-on-VLSI spatial light modulators). We compare parallel gradient descent adaptive-optics algorithms with serial gradient descent algorithms running on serially addressed modulators. While serial algorithms have previously been shown to require more iterations than parallel algorithms, we show that, because of the limitations of the databus, each serial iteration of the algorithm on a serial modulator requires significantly less time to complete than a parallel iteration, thereby favoring the serial algorithm when time to convergence is used as the performance metric. Thus, such high-resolution serially addressed devices are generally better matched to the serial-update wavefront correction algorithm owing to the data load penalty imposed by the bandwidth-limited databus of these modulators.     

Coherent acoustic vibration of metal nanoshells

Using time-resolved pump-probe spectroscopy, we have performed the first investigation of the vibrational modes of gold nanoshells. The fundamental isotropic mode launched by a femtosecond pump pulse manifests itself in a pronounced time-domain modulation of the differential transmission probed at the frequency of nanoshell surface plasmon resonance. The modulation amplitude is significantly stronger, and the period is longer than that in a gold nanoparticle of the same overall size, in agreement with theoretical calculations. This distinct acoustical signature of nanoshells provides a new and efficient method for identifying these versatile nanostructures and for studying their mechanical and structural properties.     

Fracture behavior of hybrid platelet-fiber alumina preforms

We studied the fracture behavior of alumina platelet preforms, with a global porosity of 80%, containing a weight fraction of short fibers varying between 0 and 50%. This addition of fibers leads to an increase in the work of fracture of the preform. An experimental method was set up to obtain a controlled rupture of these specimens and a load–displacement curve which allowed us to measure the increment of rupture energy due to the fiber pull-out. Experimental curves were interpreted by means of a micromechanical model developed initially for the study of the fracture behavior of ceramic matrix composites. This led us to estimate an equivalent friction stress between fibers and platelets of the order of 0.04 MPa.     

Cold storage of Pacific oysters out of water: biometry,intervalval water and sensory assessment

Due to the important economic activity of oyster production in France, microalgal toxic events represent a significant constraint for shellfish farmers who face closures of bivalve production‐sites. The frequency of closures of 3 weeks or less represents half of the total closures along the French coasts. Cold storage could be a simple and affordable solution for temporary and short‐term storage before commercialisation. A stock of marketable Pacific oyster Crassostrea gigas was therefore stored during 22 days in non‐immersed conditions at 3 °C with 100% humidity. At the end of the experiment, the oyster mortality rate remained at a low level of less than 3.5%. The sensory attributes, odour, appearance, texture and flavour, did not show significant variations. However, the oyster total weight and fresh flesh weight exhibited losses of 10.5% and a 16.6% after 15 days of storage. At the same time, intervalval water showed a decrease in quality with 20% of the samples characterized by an absence of intervalval water with a flesh more or less moist. These results suggested that the cold storage represents a partial solution to sustain the commercialisation of bivalves during closures of 15 days.     

Sensory characteristics of spoilage and volatile compounds associated with bacteria isolated from cooked and peeled tropical shrimps using SPME-GC-MS analysis

The spoilage potential of six bacterial species isolated from cooked and peeled tropical shrimps (Brochothrix thermosphacta, Serratia liquefaciens-like, Carnobacterium maltaromaticum, Carnobacterium divergens, Carnobacterium alterfunditum-like and Vagococcus penaei sp. nov.) was evaluated. The bacteria were inoculated into shrimps, packaged in a modified atmosphere and stored for 27 days at 8 °C. Twice a week, microbial growth, as well as chemical and sensory changes, were monitored during the storage period. The bacteria mainly involved in shrimp spoilage were B. thermosphacta, S. liquefaciens-like and C. maltaromaticum whose main characteristic odours were cheese-sour, cabbage-amine and cheese-sour-butter, respectively. The volatile fraction of the inoculated shrimp samples was analysed by solid-phase microextraction (SPME) and gas chromatography coupled to mass spectrometry (GC-MS). This method showed that the characteristic odours were most likely induced by the production of volatile compounds such as 3-methyl-1-butanal, 2,3-butanedione, 2-methyl-1-butanal, 2,3-heptanedione and trimethylamine.     

Three-dimensional ultrasound imaging system for prostate cancerdiagnosis and treatment

Prostate cancer is the most commonly diagnosed cancer in men in North America. Although two-dimensional (2-D) transrectal ultrasound imaging is widely used for the evaluation of prostate disease, it suffers from limitations that limit its use in diagnosis and therapy of prostate cancer. The use of conventional ultrasound requires that the diagnosticians mentally integrate a series of 2-D images in order to develop an impression of the three-dimensional (3-D) anatomy, and to estimate the volume of the prostate. This approach depends of the expertise of the physician resulting in variability. We have developed a 3-D ultrasound imaging approach that overcomes this problem. In this paper, we describe a 3-D ultrasound imaging system for use in prostate imaging and report on its performance. The system consists of a conventional ultrasound machine, a microcomputer with a video frame grabber, and a custom-built assembly for rotating the ultrasound transducer. A typical scan of 100 2-D B-mode images takes 8 s. These images are then reconstructed into a 3-D image, which can be displayed and interactively manipulated using 3-D visualization software. We also show that manual planimetry of prostates in the 3-D images can be used to estimate volumes in vitro with an accuracy of 2.6%, and a precision of 2.5%; and in vivo with 5.1% intra-observer variability and 11.4% interobserver variability. Thus, 3-D ultrasound imaging overcomes some of the limitations of conventional imaging of the prostate, and has great potential as a tool in the diagnosis and treatment of prostate disease