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31.
In a cognitive radio system, the goal is to make better use of the radio electric spectrum, allowing non-licensed users access to those currently unused electromagnetic bands assigned to licensed users (LUs). This can be achieved using OFDM, where the non-licensed users must select the temporarily available subcarriers and turn off those subcarriers used by LUs in order to avoid interference. Hence, only a subset of the subcarriers can be used for data or pilot tone transmission. To this end, some pilot allocation algorithms have been proposed for this dynamic scenario, but they are designed in such away that an equispaced pilot placement is respected (as much as possible) while minimizing the mean squared error of the channel estimate. Nevertheless, this equispaced placement can lead to the use of an increased number of pilots in order to achieve a good channel estimation. In this work, a new pilot allocation algorithm based on wavelet transform is presented. The proposed algorithm uses the discrete wavelet transform to analyze the previous channel state information, taking the knowledge of the available subcarriers into account to provide a suboptimal solution for the pilot positions. This solution leads to a non-equispaced pilot placement, which improves the channel estimation and consequently, the system performance. Likewise, the introduced algorithm allows a reduction of the number of necessary pilots, which aids in increasing the data rate. Finally, simulation results corroborate the effectiveness of the algorithm in dynamic channel scenarios.  相似文献   
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Semiconductors - Thermoelectric material development typically aims at maximizing produced electrical power and efficiency of energy conversion, even though sometimes, this means adding expensive...  相似文献   
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Water-repellent surfaces were fabricated on blend sheets of poly(l-lactic acid)/poly(butylene succinate) with various blending ratios by the successive processing; (1) plasma etching, followed by (2) the hydrophobic plasma polymer coating. Rough morphology was formed effectively on the mosaic structured surface of blend sheets via the oxidative etching, and advanced water repellency was achieved after the thin membrane coating was synthesized with a hydrophobic plasma polymer coating by use of hexamethyldisiloxane or hexamethyldisilazane. High water repellency is expressed through the columnar hair-like structured model, where the air-water surface interaction in the voids is taken into account.  相似文献   
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We sought to determine whether intracellular or extracellular events contribute to the decrease in circulating antithrombin (AT) levels that is seen in subjects with the Utah mutation (Pro 407 to Leu). Site-directed mutagenesis was used to recreate this mutation within a previously characterized rabbit AT cDNA. Cell-free expression of the mutated cDNA yielded an AT protein that failed to react with thrombin. Expression of the rabbit AT-Utah protein in transiently transfected Cos cells resulted in a 10-fold decrease in the amount of AT antigen detected in the conditioned media, as compared with that seen with the wild-type recombinant AT. This effect was not caused by variations in transfection efficiency, because AT levels were normalized to the product of a cotransfected plasmid, chloramphenicol acetyl transferase. Moreover, on Northern blot analysis, AT mRNA levels were comparable in cells expressing either the rabbit AT-Utah or wild-type recombinant rabbit AT. Immunoblots of conditioned media from the two populations of transfected cells showed that the recombinant AT-Utah protein was intact. The results obtained with Cos cells were reproduced using permanently transfected Chinese hamster ovary (CHO) cells. Pulse-chase experiments with the CHO lines showed that both initial levels of rabbit AT-Utah after the pulse labeling and the rate of subsequent secretion during the chase period were reduced compared with that seen with cells expressing the wild-type AT. The observed reduction in AT secretion was also observed for the AT-Oslo mutation (Ala 404 to Thr) when recreated in the rabbit AT background, and expressed in Cos cells. In these experiments, the media levels of mutant AT were reduced by 50%, compared with wild-type. These results show that intracellular events, as opposed to accelerated clearance or other extracellular causes, contribute to the paucity of AT secretion seen in these strand 1C AT mutants.  相似文献   
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BACKGROUND: Although strawberry aroma is very complex, certain compounds have been described as main contributors, i.e. furanones, aldehydes, alcohols, sulfur compounds and particularly methyl and ethyl esters. In addition, strawberries possess potent antioxidant activity because of their high content of phenolic compounds. Among them, flavonols are highlighted as important antioxidant compounds in strawberry. The objective of this study was to assess the effect of methyl jasmonate (MJ) on the composition of the major contributors to aroma and on the content of certain flavonols in strawberry fruits. RESULTS: The levels of all studied volatile compounds were significantly affected by MJ treatment, though the individual effect differed according to the specific compound considered. Most of them increased significantly (P < 0.05), except methyl butanoate, which always showed higher levels in untreated strawberries. In contrast to aroma compounds, the change in the concentration of flavonols (i.e. myricetin, quercetin and kaempferol) was not significant in MJ‐treated strawberries. Considering the health‐promoting activity of these compounds, further investigations on the experimental conditions related to the treatment are required to control flavonol bioformation by means of MJ. CONCLUSION: The exogenous application of MJ vapour to strawberry enhances, in general, the production of the most relevant aroma‐active compounds. On the contrary, MJ treatment does not appear to influence the levels of myricetin, quercetin and kaempferol. Thus postharvest MJ treatment is proposed as an approach to obtain improved strawberry fruits in terms of sensory quality and health‐promoting properties. Copyright © 2010 Society of Chemical Industry  相似文献   
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A front-face fluorescence spectroscopy probe was installed in the wall of a laboratory-scale cheese vat. Excitation and emission filters were chosen for the selective detection of vitamin A, tryptophan, and riboflavin fluorescence. The evolution of the fluorescence of each fluorophore during milk coagulation and syneresis was monitored to determine if they had the potential to act as intrinsic tracers of syneresis and also coagulation. The fluorescence profiles for 2 of the fluorophores during coagulation could be divided into 3 sections relating to enzymatic hydrolysis of κ-casein, aggregation of casein micelles, and crosslinking. A parameter relating to coagulation kinetics was derived from the tryptophan and riboflavin profiles but this was not possible for the vitamin A response. The study also indicated that tryptophan and riboflavin may act as tracer molecules for syneresis, but this was not shown for vitamin A. The evolution of tryptophan and riboflavin fluorescence during syneresis followed a first-order reaction and had strong relationships with curd moisture and whey total solids content (r = 0.86-0.96). Simple 1- and 2-parameter models were developed to predict curd moisture content, curd yield, and whey total solids using parameters derived from the sensor profiles (standard error of prediction = 0.0005-0.394%; R2 = 0.963-0.999). The results of this study highlight the potential of tryptophan and riboflavin to act as intrinsic tracer molecules for noninvasive inline monitoring of milk coagulation and curd syneresis. Further work is required to validate these findings under a wider range of processing conditions.  相似文献   
40.
A series of phenanthro and acenaphtho azaquinolizinium salts was selected as model heterocyclic polyaromatic compounds to study their interaction with deoxyribonucleic acid (DNA). The compounds exhibit a notable native fluorescence ( u em = 450-520 nm) in buffered aqueous solutions, as expected from planar aromatic heterocycles. However, in the presence of increasing concentrations of DNA (0, 10-90 mg/L), a loss of up to 90% of the fluorescence is observed. This fluorescence quenching can be explained by the interaction of the polycyclic ring and DNA. Application of the Stern-Volmer treatment (correlation coefficient 0.9) reveals the existence of a quenching process. The study of the interaction by UV-Vis spectrophotometry showed slight shifts (5-10 nm) in the absorption maxima of all compounds together with a decrease in the absorbance. Viscosimetry experiments confirm the association of these molecules with DNA as indicated by the increase (12-36%) of the reduced viscosity of the solutions in the presence of DNA. Equilibrium dialysis studies confirm that all compounds are bound to DNA (5-50%). These results lead to the conclusion that DNA interaction is due mainly to the carbocyclic system rather than to the heterocyclic moiety, suggesting that it takes place through hydrophobic interactions.  相似文献   
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