Preferential exclusion of sucrose from recombinant interleukin-1 receptor antagonist: role in restricted conformational mobility and compaction of native state

Understanding the mechanism for sucrose-induced protein stabilization is important in many diverse fields, ranging from biochemistry and environmental physiology to pharmaceutical science. Timasheff and Lee [Lee, J. C. & Timasheff, S. N. (1981) J. Biol. Chem. 256, 7193-7201] have established that thermodynamic stabilization of proteins by sucrose is due to preferential exclusion of the sugar from the protein's surface, which increases protein chemical potential. The current study measures the preferential exclusion of 1 M sucrose from a protein drug, recombinant interleukin 1 receptor antagonist (rhIL-1ra). It is proposed that the degree of preferential exclusion and increase in chemical potential are directly proportional to the protein surface area and that, hence, the system will favor the protein state with the smallest surface area. This mechanism explains the observed sucrose-induced restriction of rhIL-1ra conformational fluctuations, which were studied by hydrogen-deuterium exchange and cysteine reactivity measurements. Furthermore, infrared spectroscopy of rhlL-1ra suggested that a more ordered native conformation is induced by sucrose. Electron paramagnetic resonance spectroscopy demonstrated that in the presence of sucrose, spin-labeled cysteine 116 becomes more buried in the protein's interior and that the hydrodynamic diameter of the protein is reduced. The preferential exclusion of sucrose from the protein and the resulting shift in the equilibrium between protein states toward the most compact conformation account for sucrose-induced effects on rhIL-1ra.     

地面油管漏失定量测试方法探讨

对油管漏失进行定量测试的方法确定具体漏失位置。方法运用双频道回声探仪探测动液面的原理和工作方法，根据油管漏失形成的死油坏在测试曲线上出现特殊波位置判断油管漏失位置。结果：经过７０多井次的现场试验，该方法具有较高的准确性和可靠性。结论：利用特殊波可以有效监测油管漏失，并具有推广使用价值。     

带类型λ—演算

一、引言补演算是一个用函数描述计算的形式理论体系[1J,它由许多计算模型所组成。文〔1〕指出,应用认-演算比纯粹补演算更接近程序语言.但是由于内建函子和常数的引入常会发生一些问题,例如.常数O和函子+在机器中同样看待     

Evaluation of a parallel branch-and-bound algorithm on a class ofmultiprocessors

We propose and evaluate a parallel “decomposite best-first” search branch-and-bound algorithm (dbs) for MIN-based multiprocessor systems. We start with a new probabilistic model to estimate the number of evaluated nodes for a serial best-first search branch-and-bound algorithm. This analysis is used in predicting the parallel algorithm speed-up. The proposed algorithm initially decomposes a problem into N subproblems, where N is the number of processors available in a multiprocessor. Afterwards, each processor executes the serial best-first search to find a local feasible solution. Local solutions are broadcasted through the network to compute the final solution. A conflict-free mapping scheme, known as the step-by-step spread, is used for subproblem distribution on the MIN. A speedup expression for the parallel algorithm is then derived using the serial best-first search node evaluation model. Our analysis considers both computation and communication overheads for providing realistic speed-up. Communication modeling is also extended for the parallel global best-first search technique. All the analytical results are validated via simulation. For large systems, when communication overhead is taken into consideration, it is observed that the parallel decomposite best-first search algorithm provides better speed-up compared to other reported schemes     

The band areas of proteins determined by fluorescent scanning in the commercial automated gel electrophoresis apparatus

An automated gel electrophoresis apparatus, recently available commercially, allows one to follow the band during electrophoresis in real time, and lends itself therefore to an evaluation of bandwidth as a function of migration time (the dispersion coefficient), resolution and band shape. These determinations assume the constancy of band area with migration time and at various gel concentrations. The purpose of the present study was to verify these assumptions. Representative proteins and sodium dodecyl sulfate (SDS)-proteins, either natively fluorescent or fluorescein carboxylate labeled, were found to exhibit band areas which approach constancy as a function of migration time in both agarose and polyacrylamide gel electrophoresis, provided that (i) the protein concentration under the band was low enough to obviate self-quenching of fluorescence; (ii) the separation of the protein of interest from contaminants had progressed sufficiently during the time at which band areas were measured; (iii) the baseline under the peak was sufficiently well defined. However, band areas decrease with increasing gel concentration. Protein peaks exhibited leading and trailing tails. The ratio of the combined tail area to total area appeared to be near-constant at varying migration times. However, that ratio increases with increasing gel concentration. The tail area does not appear to be an artifact of fluorometric detection since it is reproduced upon fluorimetric analysis of the protein eluted from gel slices after electrophoresis. However, it may be due to photochemical destruction under the conditions of repetitive fluorometric peak detection.     

Early patterning of prelimbic cortical axons to the striatal patch compartment in the neonatal mouse

The striatum receives excitatory input from virtually the entire cerebral cortex. In the adult, this input is segregated into two functionally distinct compartments of the striatum, the patch (striosome) and matrix regions. This study determined whether the patterning of corticostriatal afferents from the prelimbic cortex to the striatal patch compartment develops during the early period of collateral formation or instead at the time of peak synaptogenesis. Initial formation of corticostriatal axon collaterals was observed by embryonic day (E) 19. Quantification of corticostriatal collaterals revealed a significant increase in the number and complexity of collateral branches at postnatal day 6 as compared to E19. Concomitant with the increase in collateral branching, a heterogeneous pattern of collateralization consisting of parallel rows of corticostriatal collaterals was observed in the medial striatum. In addition to the rows, clusters of corticostriatal axons occurred more laterally. These clusters colocalized with patches of dense tyrosine hydroxylase-positive fibers, a marker for the striatal patch compartment in the neonatal mouse. Together, these data indicate that corticostriatal patterning occurs during the period of early axon collateralization resulting in a segregation of corticostriatal axon collaterals from the prelimbic cortex to the striatal patch compartment.     

核电材料在模拟反应堆环境中应力腐蚀破裂测试技术与性能评价

简要介绍了采用慢应变速率试验，Ｕ型弯曲和Ｃ形环试验等技术，分别对８００合金，３０４和３１６及３１６Ｔｉ不锈钢Ａ５３３Ｂ压力容器用多在模拟核反应堆环境中的应力腐蚀破裂敏感性性进行的试验研究的一些主要结果；并结合电化学测试和表面膜俄歇电子能谱分析结果进行了讨论。     

Environmental-stress-screening using degradation measurements

ESS (environmental stress screening) has been extensively used to reduce infant mortality by precipitating defects. The existing ESS plans precipitate defects by stressing all products for specified durations. The plans usually require long screen durations to allow nearly all defective items to fail, and thus generate excessive aging effects on good items. For some products, failures are defined in terms of performance characteristics exceeding their critical values. This paper describes the principles of using degradation measurements of performance characteristics to screen the products. In an ESS, the performance characteristics of defective items degrade considerably faster than those of good ones, yielding a bimodal distribution of the characteristics. As screen duration increases, the 2 modes of the distribution shift apart. It is possible to find a tightened critical value to weed out the defective items before they fail. This paper, based on these principles, further designs the optimal 2-level screen plans which minimize a segment of life-cycle cost and which simultaneously meet the reliability requirement. The minimum cost is achieved by choosing optimal part-level and unit-level screen durations and tightened critical values of parts. A numerical example is followed by discussion. Because the ESS regime of this paper allows defective parts to be screened out before they fail, the developed optimal ESS plans can reduce life-cycle cost, shorten part-level screen duration, and alleviate aging effects on good products     

8Li次级束的产生

在北京串列加速器次级束流线上通过2 H(7Li,8Li) 1H逆运动学反应产生了用于核天体物理研究的8Li次级束。准直后的次级束纯度达到 80 %以上 ,对于 44MeV的7Li强度约为 3 0s- 1·pnA- 1,可以用来进行逆几何转移反应的实验测量。