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41.
Membrane proteins: from sequence to structure 总被引:12,自引:0,他引:12
The prediction of protein structure from sequence has been along-standing goal of molecular biology. Integral membrane proteins,once abhorred by protein chemists and crystallographers becauseof their insolubility and stubborn refusal to yield good crystals,now appear to hold great promises for efficient structure predictionand engineering. This is mainly due to the constraints on permissiblestructures imposed by the lipid environment, and to the apparentuncoupling between an initial membrane targeting and insertionprocess which determines the overall topological arrangementof the transmembrane segments and a subsequent condensationof these segments into a unique folded state. Recent work suggeststhat the membrane insertion process is controlled by simplesequence elements composed of different combinations of longhydrophobic regions and flanking charged residues. In this reviewwe sketch the most unportant structural rules relating aminoacid sequence to membrane insertion to fully folded molecule,and their use for prediction and protein-engineering purposes. 相似文献
42.
Matthew F. Johnson Colin R. Thorne Janine M. Castro G. Mathias Kondolf Celeste Searles Mazzacano Stewart B. Rood Cherie Westbrook 《河流研究与利用》2020,36(1):3-12
River management based solely on physical science has proven to be unsustainable and unsuccessful, evidenced by the fact that the problems this approach intended to solve (e.g., flood hazards, water scarcity, and channel instability) have not been solved and long‐term deterioration in river environments has reduced the capacity of rivers to continue meeting the needs of society. In response, there has been a paradigm shift in management over the past few decades, towards river restoration. But the ecological, morphological, and societal benefits of river restoration have, on the whole, been disappointing. We believe that this stems from the fact that restoration overrelies on the same physical analyses and approaches, with flowing water still regarded as the universally predominant driver of channel form and structural intervention seen as essential to influencing fluvial processes. We argue that if river restoration is to reverse long‐standing declines in river functions, it is necessary to recognize the influence of biology on river forms and processes and re‐envisage what it means to restore a river. This entails shifting the focus of river restoration from designing and constructing stable channels that mimic natural forms to reconnecting streams within balanced and healthy biomes, and so levering the power of biology to influence river processes. We define this new approach as biomic river restoration. 相似文献
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Cancer treatment is a fragmented and varied process, as “cancer” is really hundreds of different diseases. The “hallmarks of cancer” proposed by Hanahan and Weinberg (Cell 100(1):57–70, 2000) are a framework for viewing cancer within a common set of underlying principles—ten properties that are common to almost all cancers, allowing them to grow uncontrollably and ravage the body. We used a cellular automaton model of tumour growth paired with lattice Boltzmann methods modelling oxygen flow to simulate combination drugs targeted at knocking out pairs of hallmarks. We found that knocking out some pairs of cancer-enabling hallmarks did not prevent tumour formation, while other pairs significantly prevent tumour growth (\(p=0.0004\) using Wilcoxon signed-rank adjusted with the Bonferroni correction for multiple comparisons). This is not what would be expected from models of knocking out the hallmarks individually, as many pairs did not have an additive effect but had either no statistically significant effect or a multiplicative one. We propose that targeting certain pairs of cancer hallmarks, specifically cancers ability to induce blood vessel development paired with another cancer hallmark, could prove an effective cancer treatment option. 相似文献
46.
Younes Seifi Suriadi Suriadi Ernest Foo Colin Boyd 《International Journal of Information Security》2015,14(3):221-247
To prevent unauthorized access to protected trusted platform module (TPM) objects, authorization protocols, such as the object-specific authorization protocol (OSAP), have been introduced by the trusted computing group (TCG). By using OSAP, processes trying to gain access to the protected TPM objects need to prove their knowledge of relevant authorization data before access to the objects can be granted. Chen and Ryan’s 2009 analysis has demonstrated OSAP’s authentication vulnerability in sessions with shared authorization data. They also proposed the Session Key Authorization Protocol (SKAP) with fewer stages as an alternative to OSAP. Chen and Ryan’s analysis of SKAP using ProVerif proves the authentication property. The purpose of this paper was to examine the usefulness of Colored Petri Nets (CPN) and CPN Tools for security analysis. Using OSAP and SKAP as case studies, we construct intruder and authentication property models in CPN. CPN Tools is used to verify the authentication property using a Dolev–Yao-based model. Verification of the authentication property in both models using the state space tool produces results consistent with those of Chen and Ryan. 相似文献
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There is a wish to be able to enter text into mobile computing devices at the speed of speech. Only handwritten shorthand schemes can achieve this data recording rate. A new, overall solution to the segmentation and recognition of phonetic features in Pitman shorthand is proposed in this paper. Approaches to the recognition of consonant outlines, vowel and diphthong symbols and shortforms, which are different components of Pitman shorthand, are presented. A new rule is introduced to solve the issue of smooth junctions in the consonant outlines which was normally the bottleneck for recognition. Experiments with a set of 1127 consonant outlines, 2039 vowels and diphthongs and 841 shortforms from three shorthand writers have demonstrated that the proposed solution is quite promising. The recognition accuracies for consonant outlines, vowels and diphthongs, and shortforms achieved 75.33%, 96.86% and 91.86%, respectively. From the evaluation of 461 outlines with smooth junction, the introduction of the new rule has a great positive effect on the performance of the solution. The recognition accuracy of smooth junction improves from 37.53% to 93.41% given a writing time increase of 14.42%. 相似文献
49.
Colin Ware 《Computer Graphics and Applications, IEEE》2008,28(2):6-11
Currently, most researchers in visualization pay very little attention to vision science. The exception is when the effective use of color is the subject. Little research in flow visualization includes a discussion of the related perceptual theory. Nor does it include an evaluation of effectiveness of the display techniques that are generated. This is so, despite Laidlaw's paper showing that such an evaluation is relatively straightforward. Of course, it's not always necessary to relate visualization research to perceptual theory. If the purpose of the research is to increase the efficiency of an algorithm, then the proper test is one of efficiency, not of perceptual validity. But when a new representation of data is the subject of research, addressing how perceptually effective it is - either by means of a straightforward empirical comparison with existing methods or analytically, relating the new mapping to perceptual theory - should be a matter of course. A strong interdisciplinary approach, including the disciplines of perception, design, and computer science will produce better science and better design in that empirically and theoretically validated visual display techniques will result. 相似文献
50.
Colin B. Macdonald Sigal Gottlieb Steven J. Ruuth 《Journal of scientific computing》2008,36(1):89-112
Diagonally split Runge–Kutta (DSRK) time discretization methods are a class of implicit time-stepping schemes which offer
both high-order convergence and a form of nonlinear stability known as unconditional contractivity. This combination is not
possible within the classes of Runge–Kutta or linear multistep methods and therefore appears promising for the strong stability
preserving (SSP) time-stepping community which is generally concerned with computing oscillation-free numerical solutions
of PDEs. Using a variety of numerical test problems, we show that although second- and third-order unconditionally contractive
DSRK methods do preserve the strong stability property for all time step-sizes, they suffer from order reduction at large
step-sizes. Indeed, for time-steps larger than those typically chosen for explicit methods, these DSRK methods behave like
first-order implicit methods. This is unfortunate, because it is precisely to allow a large time-step that we choose to use
implicit methods. These results suggest that unconditionally contractive DSRK methods are limited in usefulness as they are
unable to compete with either the first-order backward Euler method for large step-sizes or with Crank–Nicolson or high-order
explicit SSP Runge–Kutta methods for smaller step-sizes.
We also present stage order conditions for DSRK methods and show that the observed order reduction is associated with the
necessarily low stage order of the unconditionally contractive DSRK methods.
The work of C.B. Macdonald was partially supported by an NSERC Canada PGS-D scholarship, a grant from NSERC Canada, and a
scholarship from the Pacific Institute for the Mathematical Sciences (PIMS).
The work of S. Gottlieb was supported by AFOSR grant number FA9550-06-1-0255.
The work of S.J. Ruuth was partially supported by a grant from NSERC Canada. 相似文献