Real-time assessment of fluid flow generated by appendage movements of Daphnia using standing square-wave chronamperometry

Standing square-wave chronoamperometry (SSWCA) was applied to the analysis of the microfluid flow generated by the movement of the appendages of the Crustacea Daphnia. This novel approach provided for the first time real-time assessment and analysis of the breathing rate/fluid flow of individual organisms. An electrochemical tracer was delivered into the fluid inflow of the organism and a carbon fiber microelectrode placed in the fluid outflow's path. The variation of the net concentration/flux of the electroactive tracer, dopamine, at the electrode surface was measured with SSWCA. The observed chronoamperometric peaks (with fine structure) of the outflow are seen as a direct representation of appendage movement and, too, the workings and responses of the organism to its environment, e.g., external stimuli such as food or chemicals. It was concluded that SSWCA follows primarily the variation of the convective component of the Nernst-Plank equation for flux and, to lesser extent, diffusion and migration. In this work, SSWCA can clearly be used to monitor changes in the Daphnia-generated fluid outflow on a different time scale than was previously possible. This new application of SSWCA is faster and likely more accurate than using high-speed video.     

Study of condition-dependent decomposition reactions; Part I. The thermal behaviour and decomposition of 2-nitrobenzoyl chloride

The risks associated with batch processing in the manufacture of chemicals and pharmaceuticals via highly exothermic reactions are of special interest due to the possibility of runaway reactions. o-Nitrated benzoyl chlorides are intermediates in the production of agrochemicals and are produced via the reaction of o-nitrated carboxylic acids with thionyl chloride in a solvent mixture. ortho-Nitrated acyl chlorides have exploded violently on attempted distillation on numerous occasions. An inadequate investigation of the process prior to large-scale operation is the most likely cause. Here we present preliminary results of studies on the decomposition of 2-nitrobenzoyl chloride. This study has revealed that the decomposition reaction is strongly condition dependent. The heating rate of the sample plays a preponderant role in the course of the decomposition reaction. That renders the interpretation of differential scanning calorimetry (DSC) or adiabatic calorimetry measurements, which are routinely used to assess the thermochemistry and safety of the large-scale reactions, problematic. Following this on-going study, we report here key features of the system that have been identified.     

The metrology of a miniature FT spectrometer MOEMS device using white light scanning interference microscopy

Micro-optical electro-mechanical systems (MOEMS) technology, making use of existing silicon based fabrication techniques shows great potential for making complete miniaturized hybrid devices. Such technology has been used to make a Fourier transform spectrometer based on a time-scanning Michelson interferometer. An electrostatic comb drive actuator moves the scanning mirror over a distance of 40 μm. The measured resolution of the spectrometer is 6 nm at a wavelength of 633 nm. The dimensions of the device are 5×5×0.5 mm, and the depth of feature is 75 μm. During quality control of such devices it is necessary to check the dimensions of micron wide structures that are tens of microns deep, over areas of tens of square millimeters. In this work we have investigated the use of white light scanning interference (WLSI) microscopy for making rapid, non-destructive precision three-dimensional measurements. While a high axial precision can be achieved, an artifact has been observed with classical configurations that tend to extend the location of deep step discontinuities by up to 3 μm and so broaden narrow structures. With certain modifications in the optical configuration, this error can be considerably reduced. The results of this work demonstrate that WLSI shows great potential for the rapid and precise quality control of MOEMS devices.     

Brute Force Polarization of 129Xe

Hyperpolarized ¹²⁹Xe has numerous applications in Nuclear magnetic resonance (NMR) and magnetic resonance imaging(MRI). As an alternative to the laser optical pumping method of production of hyperpolarized ¹²⁹Xe gas, we have investigated the brute force technique which uses a very high magnetic field and millikelvin temperatures. One obstacle to this technique is the extremely long spin lattice relaxation times of nuclei in solids at very low temperature. We exploit the fact that liquid ³He can be used as an effective relaxant to enable ¹²⁹Xe to be spin polarized on a high surface area substrate in a few hours. We are able to identify the contributions of different atomic layers to the magnetization by analyzing the ¹²⁹Xe NMR spectrum. Furthermore, the addition of ⁴He allows us to turn off the relaxation mechanism, thereby preserving the polarization. The technique could be applied to nuclei other than ¹²⁹Xe.     

Interfacial reactions in Mo-Zr multilayer structure: an X-ray reflectivity study

The effect of elevated temperature on the structural stability and alloy formation in Mo-Zr multilayers is investigated. Mo-Zr multilayers deposited by the electron beam evaporation technique under ultra-high vacuum conditions are annealed up to 650 °C. The changes induced due to thermal treatment are observed using X-ray reflectivity (both specular and off-specular) and X-ray diffraction techniques. The Mo-Zr multilayers remained as an insoluble layered structure even after annealing as revealed from X-ray reflectivity measurements. The interfacial roughness is found to be very similar at all interfaces and decreases on annealing. The multilayer structure remains intact on annealing with expansion of the multilayer period and a marginal increase in X-ray reflectivity.     

Computational modelling of multiscale morphologies in polymer-liquid crystal blends

Simulations of material architectures in polymer-liquid crystal blends driven by phase separation-phase ordering-texturing processes are presented. The study shows that mixtures of polymers and liquid crystals result in blend morphologies that organize at several scales. For thermally driven instabilities, morphologies of polymer droplets embedded in a liquid crystal matrix show colloidal crystallinity. Large polymer drops strongly affect the orientation of the matrix, producing textures consisting of defect lattices. This work shows that thermally driven phase separation-phase ordering-texturing processes can result in multiscale materials, with length scales cascading down from droplets to interfaces, and finally to nanoscale defects.